The aim of this study was to evaluate the outcome of N-acetyl-L-cysteine (NAC), as an antioxidant on semen quality, protamine deficiency, DNA fragmentation, reactive oxygen species (ROS) and fertility in individuals with varicocele following varicocelectomy. The study included 35 infertile men with varicocele grade II and III who were referred to Isfahan fertility and infertility center for varicocelectomy.This study was designed similar to a double blind clinical trial that patients and researchers were blinded to type of treatment. This individuals were randomly divided into control (n=20) and NAC (n=15) groups. In the control group, the individuals received placebo post varicocelectomy. In the treatment group or NAC group, the individuals received three tablets of NAC post varicocelectomy (200 mg daily) for three months. Semen parameters (according World Health Organization (WHO) criteria), protamine content (Chromomycin A3), DNA Fragmentation (TUNEL) and ROS statues (DCFH-DA and flow cytometery) were assessed before and three months after varicocelectomy.
General information
Acronym
IRCT registration information
IRCT registration number:IRCT201508177223N5
Registration date:2015-10-19, 1394/07/27
Registration timing:retrospective
Last update:
Update count:0
Registration date
2015-10-19, 1394/07/27
Registrant information
Name
Mohammad Hossein Nasr-Esfahani
Name of organization / entity
Royan Institute (Royan Institute for Biotechnology)
Country
Iran (Islamic Republic of)
Phone
+98 31 9501 5688
Email address
mh_nasr@med.mui.ac.ir
Recruitment status
Recruitment complete
Funding source
Royan Institute
Expected recruitment start date
2012-05-14, 1391/02/25
Expected recruitment end date
2014-07-02, 1393/04/11
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Evaluation of the effect of N-Acetyl cysteine on sperm parameters, sperm chromatin and oxidative s
Public title
The effect of N-Acetyl cysteine on sperm function, after Varicocelectomy
Purpose
Health service research
Inclusion/Exclusion criteria
Inclusion criteria: Infertile men with primary infertility and left-sided varicocele (grade II and III) and without female factor infertility.
Exclusion criteria: Men with grade I varicocele, Azoospermia, leukocytospermia, anatomical disorders, consumption of drug and occupational exposure