The role of physical activity with diet to lose weight is clear . The purpose of this study was to evaluate the effect of high and low frequency of physical activity on weight loss in healthy obese and overweight women . For this purpose, 60 eligible healthy obese and overweight women(Body mass index 27-35 kg/m2), age 18-50 years, no regular exercise,no self-report of acute or chronic disease, no evidence of amenorrhea were selected and divided into two groups (n=30); 1) physical activity with high frequency ( 6 days / 50 min ) and 2 ) physical activity with low frequency ( 3 days / 100 min ). Participants are involved in other weight loss programs with dietary regimen intervention as a component, are anemic (Hematocrit < 34%), report total cholesterol > 300 mg/dl, report triglyceride > 350 mg/dl will be excluded. During the study period, all of the participants will follow a diet with caloric restriction ( 500 kcal less than BMR). Before and after the study, blood parameters including triglycerides, total cholesterol, HDL, LDL, fibrinogen, and HOMA-IR are measured. Weight and waist circumference is measured at the beginning of the study and every two weeks . Physical activity adherence is controlled by pedometer.