IRCT2013050813267N1 IRCT 2014-02-06 Shahid Sadoughi Yazd University Of Medical Sciences, Vice Chancellor for Research Studying the effect of bone marrow mesenchymal stem cell injection into the subarachnoid space on improvement and reduction of complications in spinal cord injured patients Studying the effect of bone marrow mesenchymal stem cell injection into the subarachnoid space on improvement and reduction of complications in spinal cord injured patients 2016-12-05 anticipated 30 Complete https://irct.ir/trial/13181 interventional Randomization: Randomized, Blinding: Single blinded, Placebo: Not used, Assignment: Parallel, Purpose: Treatment. 1-2 Spinal Cord Injury. Intervention 1: In intervention group, at first bone marrow aspiration (10ml) will be done and mesenchymal stem cells will be collected using centrifuge and Ficoll solvent. Cells will be cultured and after proliferation they will be collected by trypsination and microbial, quality and quantity analysis will be done on them. Collected cells with the quantity of 10-20 million cells will be solved in 5ml distilled water, and will be injected slowly (5 minutes) to sub arachnoid space of patient using lumbar puncture technique by anesthesiologist in operating room and in sterile conditions. Each patients will have 3 injections with near to 1 month interval between them. Intervention 2: In control group just vehicle of cells (distilled water) with the same volume of intervention group (5ml) will be injected to the sub arachnoid space of patients. Injecting solvent will be injected slowly (5 minutes) and by anesthesiologist in the operating room and in sterile conditions. Each patient in this group will receive 3 injections by one month interval between them. Bone Marrow aspiration will not perform in this group but just the action of bone marrow aspiration will be simulated in the operating room for them.