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IRCT2014121620329N1 IRCT 2015-08-26 Investigator Effect of Wet Cupping on Blood Components of Healthy volunteers A Metabonomics Study of the Effect of Wet Cupping on Blood Components of Healthy Volunteers 2015-05-01 anticipated 20 Complete https://irct.ir/trial/18036 interventional Randomization: N/A, Blinding: Not blinded, Placebo: Not used, Assignment: Single, Purpose: Other, Other design features: Venous blood before cupping, cupping blood and venous blood 2 weeks after cupping in healthy volunteers were analyzed by auto analyzer and 1HNMR. Using modelling software like MATLAB differentiating metabolites between these three samples were detected and identified and the difference in the metabolic cycles between these three were also analyzed. 3 Healthy condition. Intervention : Wet cupping using scalpel and suction on a region between the scapula.
public Dr.saeed Bouzari
no: 1996, Pasteur Ave. Pasteur Sq.
Tehran Iran (Islamic Republic of) 1316943551 +98 21 6640 2770 bouzari@pasteur.ac.ir Pasteur Institute of Iran Vice chancellor for research scientific Dr. Zahra Zamani
Pasteur Ave.
Tehran Iran (Islamic Republic of) 1316943551 +98 21 6640 2770 zamani@pasteur.ac.ir Pasteur Institute of Iran Iran (Islamic Republic of) Inclusion criteria: Healthy male 20-70 years old volunteers, who come to traditional medicine clinic for routine annual wet cupping. Exclusion criteria: Chronic disease patients and patients under medication 20 years 70 years Male Treatment - Other Intervention : Wet cupping using scalpel and suction on a region between the scapula Metobonomics of venous blood and cupping blood. Timepoint: On the same day before and after cupping, 2 weeks later. Method of measurement: Using 1H Nuclear Magnetic Resonance by NMR apparatus. Triglyceride. Timepoint: On the same day between venous blood and cupping blood , 2 weeks later between first and second venous blood. Method of measurement: With the use of biochemical kits (Pars Azmoon) and autoanalyzer RA 1000. High density lipoprotein (HDL). Timepoint: On the same day before and after cupping, 2 weeks later. Method of measurement: Using biochemical kits (Pars Azmoon) and autoanalyzer RA 1000. Low density lipo proteins (LDL). Timepoint: On the same day before and after cupping, 2 weeks later. Method of measurement: Using biochemical kits(Pars Azmoon) and autoanalyzer RA 1000. Uric acid. Timepoint: On the same day before and after cupping, 2 weeks later. Method of measurement: Using biochemical kits (Pars Azmoon) and autoanalyzer RA 1000. Liver enzymes. Timepoint: On the same day before and after cupping, 2 weeks later. Method of measurement: Using biochemical kits (Pars Azmoon) and autoanalyzer RA 1000. Investigator Approved 2012-03-25 Human Research Ethics Committee of Pasteur Institute of Iran 12th. St., the eastern Pasteur Ave., Pasteur Sq.,Valiasr Ave. Pasteur institute of Iran Tehran Iran (Islamic Republic of)