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IRCT2016020726409N1 IRCT 2016-03-27 Pishgaman Armaghan Aftab Company Evaluation of Herbal Spray as hands Antiseptic Spray A-S14-1 Evaluation of antiseptics effect of herbal solution of A-S14-1 on hand skin 2016-01-20 anticipated 20 Complete https://irct.ir/trial/21902 interventional Randomization: N/A, Blinding: Not blinded, Placebo: Not used, Assignment: Single, Purpose: Prevention, Other design features: -. N/A Topical Agent. Effect of herbal solution A-S14-1 product of Pishgaman Armaghan Aftab Company on microbial count of fingers tips will compare before and after washing with the solution as EN12791 procedure. The herbal solution of A-S14-1 is compatible with skin, without any alcohol and chemicals such as chlorinated compounds, silver nanoparticles, tetravalent ammonium compounds, Triclosan etc. Antisepsis of both hands will carry out by 3 ml of A-S14-1 solution and rubbing. If necessary, an additional 3 ml will add. As described for basic Microbial count, the method will accomplish on randomly selected hand after 3 minutes antisepsis by studied solution. The other hand will cover with a sterile surgical glove..
public Ghasem Habibi Dehfooli
No. 14, Molaem, Besharat St, Arak, Iran
Arak Iran (Islamic Republic of) 381875875 +98 86333664708 ghasemhabibi.chem@gmail.com Pishgaman Armaghan Aftab Company scientific Professor Parviz Owlia
Molecular Microbiology Research Center, Shahed University, Tehrsn, Iran
Tehran Iran (Islamic Republic of) +98 21 5522 8800 Owlia@Shahed.ac.ir Shahed University Iran (Islamic Republic of) Inclusion criteria: Fingernails should be short and clean; Non-use of ointments, medications or antimicrobial soap for at least a week before the interventions Exclusion criteria: Pregnant women; There are cuts, wounds, abrasions on the skin of the hands; The risk of skin disease 18 years 100 years Both Y56.9 Topical agent, unspecified Prevention Effect of herbal solution A-S14-1 product of Pishgaman Armaghan Aftab Company on microbial count of fingers tips will compare before and after washing with the solution as EN12791 procedure. The herbal solution of A-S14-1 is compatible with skin, without any alcohol and chemicals such as chlorinated compounds, silver nanoparticles, tetravalent ammonium compounds, Triclosan etc. Antisepsis of both hands will carry out by 3 ml of A-S14-1 solution and rubbing. If necessary, an additional 3 ml will add. As described for basic Microbial count, the method will accomplish on randomly selected hand after 3 minutes antisepsis by studied solution. The other hand will cover with a sterile surgical glove. Basic Microbial count. Timepoint: Before Antisepsis by studied herbal solution. Method of measurement: Volunteers wash their hands with liquid normal soap for a minute and then rinse and drying laundry, The fingertips of both hands will rub for 1 minute in a petri dish containing 10 ml of TSB. Then,1 and 0.1 ml of the liquid will take and will culture on petri dishes containing TSA (with a glass rod head tilt). In addition, from the fluid, one-tenth dilution in TSB was prepared and 0.1 ml will culture on TSA. If necessary, microbial count will continue to further dilutions in a pour-plate method. All samples will incubate at a temperature of 37±1 for 48-24 hours and then cfu / ml. Microbial count immediately after antisepsis by studied herbal solution. Timepoint: immediately after antisepsis by studied herbal solution. Method of measurement: Antisepsis of both hands will carry out by 3 ml of A-S14-1 solution and rubbing. If necessary, an additional 3 ml will add. As described for basic Microbial count, the method will accomplish on randomly selected hand after 3 minutes antisepsis by studied solution. The other hand will cover with a sterile surgical glove. Microbial count 3 hours after antisepsis by studied herbal solution. Timepoint: 3 hours after antisepsis by studied herbal solution. Method of measurement: After 3 hours the glove from hand will remove and microbial count will perform as earlier mentioned method. ND ND Pishgaman Armaghan Aftab Company Approved 2016-01-19 Shahed University, Depaty of Research Behesht-Zahra, Khalije-Fars Freeway, Tehran Tehran Iran (Islamic Republic of)