IRCT2016082429508N1 IRCT 2016-09-20 Vice chancellor for research, Urmia University of Medical Sciences The effect of hypocaloric diet and omega-3 fatty acid supplementation on overweight and obesity The effect of a hypocaloric diet and omega-3 fatty acid supplementation on serum levels of liver enzymes, lipid profile and insulin resistance in overweight and obese patients with non-alcoholic fatty liver disease 2016-09-22 anticipated 114 Complete https://irct.ir/trial/23698 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Treatment. 2 Non-alcoholic fatty liver disease. Intervention 1: First intervention group: Thirty eight patients with Nafld will receive a hypocaloric diet program consisting of 50% carbohydrates, 20% protein and 30% fat with a goal of 5% weight reduction. Dietary intake will assess by 24-hour recall method for 3 days and food frequency questionnaire at baseline and the end of the trial. Anthropometric measurements, fasting blood sugar, fasting insulin, lipid profile (TC, LDL-C, HDL-C, TG), HOMA-IR, liver enzyme (ALT and AST) and physical activity data will be collected at baseline and the end of the intervention. Intervention 2: Second intervention group: Thirty eight patients with Nafld will take 2 omega-3 fatty acids capsules per day providing 1500 mg EPA+DHA. Dietary intake will assess by 24-hour recall method for 3 days at baseline and the end of the trial. Anthropometric measurements, fasting blood sugar, fasting insulin, lipid profile (TC, LDL-C, HDL-C, TG), HOMA-IR, liver enzyme (ALT and AST) and physical activity data will be collected at baseline and the end of the interventio. Intervention 3: Control group: no intervention.