IRCT2017021431250N2 IRCT 2017-03-07 Vice chancellor for research, Islamic Azad University of Medical Science Dental School The Effect of the Titanium nitride coating on bacterial adhesion on orthodontic stainless steel wires The Effect of the Titanium nitride coating on bacterial adhesion on orthodontic stainless steel wires: invivo study 2016-01-02 anticipated 20 Complete https://irct.ir/trial/24624 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Prevention. 2-3 Bacterial adhesion on TiN coated orthodontic stainless steel wire. Intervention 1: 19*25 Stainless steel archwires will be evaluated, each patients will be their own control, the coated archwire will be assigned to one jaw randomly. After 4 weeks, the archwires will be removed and cut at similar lengths of 20 mm. they will be placed in sterile dishes containing saline phosphate buffer and sent to the lab for bacterial colony counting that includes dilution and culture on blood agar plates and eventually counting the colonies. each sample will be counted 3 times and the average number will be recorded. Intervention 2: The opposing jaw as a control group will be recieve a non-coated 19*25 Stainless steel archwire. After 4 weeks, the archwires will be removed and cut at similar lengths of 20 mm. they will be placed in sterile dishes containing saline phosphate buffer and sent to the lab for bacterial colony counting that includes dilution and culture on blood agar plates and eventually counting the colonies. each sample will be counted 3 times and the average number will be recorded.