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IRCT20141217020351N10 IRCT 2018-05-13 Tehran University of Medical Sciences The sequential embryo transfer compared to single embryo transfer in one cycle The sequential embryo transfer in the third and fifth day compared to single embryo transfer in the fifth day in IVF cycle 2015-04-09 anticipated 130 Complete https://irct.ir/trial/29988 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Treatment, Randomization description: they were allocated to one of two groups via simple random sampling, using a random Pocket (intervention or Control)by the same coordinator.Patients were allocated to either sequential transfer (day 3 and day 5) or conventional transfer (day 5) protocols. 3 Infertility,Repeated implantation failure,ٍEmbryo transfer,Blastocyst. Intervention 1: Intervention group: patients were randomized to one of the 2 groups of Intervention and control .After ovulation induction, ٌWhen 3leading follicles reached more than 17 mm diameter means, oocyte retrieval was performed 36 hours later under the unconscious sedation. The retrieved oocytes were incubated in a mini-incubator with the triple gas mixture for 3h in a fertilization medium and then ICSI was done. Injected oocytes were transferred to a culture dish with cleavage medium (Sydney IVF cleavage medium, cook ). Fertilization was assessed 16-18 h after ICSI and then observed for cleavage on day 3. Once post-fertilization check confirmed availability of ≥5 embryos, . In the third day after injection, embryo quality was evaluated, and 1 good-quality embryos were transferred (Cleavage in fresh cycles). Also, embryos were transferred to Blastocyst medium (Sydney IVF blastocyst medium) for 48 h. Then, on the fifth day, two embryos (Blastocyst) were transferred to the uterus. Intervention 2: Control group: After ovulation induction, ٌWhen 3leading follicles reached more than 17 mm diameter means, oocyte retrieval was performed 36 hours later under the unconscious sedation. The retrieved oocytes were incubated in a mini-incubator with the triple gas mixture for 3h in a fertilization medium and then ICSI was done. Injected oocytes were transferred to a culture dish with cleavage medium (Sydney IVF cleavage medium, cook ). Fertilization was assessed 16-18 h after ICSI and then observed for cleavage on day 3. Once post-fertilization check confirmed availability of ≥5 embryos, In the control group, on the fifth day, two embryos (Blastocyst) were transferred to the uterus. No - There is not a plan to make this available Justification or reason for not sharing IPD is Maintaining privacy in patient data
public Ensieh Shahrokh Tehraninejad
Vali Asr Infertility Clinic,Imam Khomeini Hospital,Keshavarz Blvd.
Tehran Iran (Islamic Republic of) 14194 +98 21 6658 1616 etehraninejad@sina.tums.ac.ir Tehran University of Medical Sciences scientific Ensieh Shahrokh Tehraninejad
Vali Asr Infertility Clinic ,Imam Khomeini Hospital,Keshavarz BLvd.
Tehran Iran (Islamic Republic of) 14194 +98 21 6658 1616 etehraninejad@sina.tums.ac.ir Tehran University of Medical Sciences Iran (Islamic Republic of) age ≤ 40 years normal karyotype normal screening of immunological and thrombolytic condition the absence of endometrial abnormalities by hysteroscopy availability of ≥5 embryos 18 years 40 years Female potential poor responders Ovarian hyper stimulation response presence of medical diseases N97 Female infertility Treatment - Surgery Treatment - Other Intervention group: patients were randomized to one of the 2 groups of Intervention and control .After ovulation induction, ٌWhen 3leading follicles reached more than 17 mm diameter means, oocyte retrieval was performed 36 hours later under the unconscious sedation. The retrieved oocytes were incubated in a mini-incubator with the triple gas mixture for 3h in a fertilization medium and then ICSI was done. Injected oocytes were transferred to a culture dish with cleavage medium (Sydney IVF cleavage medium, cook ). Fertilization was assessed 16-18 h after ICSI and then observed for cleavage on day 3. Once post-fertilization check confirmed availability of ≥5 embryos, . In the third day after injection, embryo quality was evaluated, and 1 good-quality embryos were transferred (Cleavage in fresh cycles). Also, embryos were transferred to Blastocyst medium (Sydney IVF blastocyst medium) for 48 h. Then, on the fifth day, two embryos (Blastocyst) were transferred to the uterus. Control group: After ovulation induction, ٌWhen 3leading follicles reached more than 17 mm diameter means, oocyte retrieval was performed 36 hours later under the unconscious sedation. The retrieved oocytes were incubated in a mini-incubator with the triple gas mixture for 3h in a fertilization medium and then ICSI was done. Injected oocytes were transferred to a culture dish with cleavage medium (Sydney IVF cleavage medium, cook ). Fertilization was assessed 16-18 h after ICSI and then observed for cleavage on day 3. Once post-fertilization check confirmed availability of ≥5 embryos, In the control group, on the fifth day, two embryos (Blastocyst) were transferred to the uterus. Chemical pregnancy. Timepoint: Two weeks after the stopping the menstruation. Method of measurement: Blood sample for B-HCG. Clinical Pregnancy. Timepoint: Six weeks after seeing the embryo sac in ultrasound. Method of measurement: Ultrasonography. Abortion. Timepoint: After positive pregnancy test. Method of measurement: Blood sample of B-HCG. Ectopic pregnancy. Timepoint: After the positive of pregnancy test. Method of measurement: Sonography. Tehran University of Medical Sciences Approved 2017-07-11 Ethics committee of Tehran University of Medical Sciences No.6,Ghods Ave,Keshavarz Blvd,Deputy of Research of Tehran University of Medical Sciences Tehran Tehran Iran (Islamic Republic of)