IRCT201601173690N6 IRCT 2016-03-13 Gingival and dental research center of Tabriz dentistry faculty Effect of adjunctive locally delivered doxycycline microspheres on clinical parameters and the gingival crevicular fluid matrix metalloproteinase-8 levels in chronic periodontitis Effect of adjunctive locally delivered doxycycline microspheres on clinical parameters and the gingival crevicular fluid matrix metalloproteinase-8 levels in chronic periodontitis 2016-02-18 anticipated 25 Complete https://irct.ir/trial/3787 interventional Randomization: Randomized, Blinding: Single blinded, Placebo: Not used, Assignment: Parallel, Purpose: Treatment. N/A Chronic periodontitis. Intervention 1: Test group: Initial sampling was carried out before every intervention to determine mmp8 level of gingival crevicular fluid and evaluate clinical parameters including probe depth, attachment level and bleeding of probing. Hence,the treatment was periodontal non surgical(i.e.Scailing and Root planing ) 10% local doxycycline was placed , instantly, inside the pocket in the test group.1-3months later the sampling and evaluation of clinical parameters were repeated. Intervention 2: Control groupInitial sampling was carried out before every intervention to determine mmp8 amount of Gingival Crevicular fluid and evaluate clinical parameters including probe depth, Attachment level and bleeding of probing. Hence, the treatment was periodontal non-surgical (i.e. Scailing and Roo planing). 1-3 months later the sampling and evaluation of clinical parameters were repeated.