IRCT201604304076N19 IRCT 2016-06-27 Resaerch Deputy of Tehran University of Medical Sciences Evaluation of the Response to Treatment of HIV-1 Infected Men's Semen Sperm Washing Method in Pregnancy of Sero-Discordant Couples Evaluation of the Response to Treatment of HIV-1 Infected Men's Semen Sperm Washing Method in Pregnancy of Sero-Discordant Couples who Referred to Behavioral Diseases Consultation Center 2016-04-03 anticipated 15 Complete https://irct.ir/trial/4263 interventional Randomization: N/A, Blinding: Not blinded, Placebo: Not used, Assignment: Single, Purpose: Prevention. N/A Condition 1: HIV/AIDS. Condition 2: Pregnancy, childbirth and the puerperium. Intervention 1: Intervention group: In this method, healthy and motile uninfected sperm cells are isolated from other cells. Semen samples will be loaded on a sperm washing medium gradient and then centrifuged. Finally healthy and motile uninfected sperm cells will deposit. For the next step, sperm pellet will be load on the bottom of a sterile tube which is filled with Ham's F10 and will be incubate for 30 minutes in 37 C temperature. Healthy and motile sperm cells will swim to the upper surface of medium, and concentrate there and will be isolated for next step. For evaluating of the effectiveness of the sperm washing, viral load counting (Real Time PCR) will be done on a part of the washed sperm pellet. If the test's result is reported negative (Undetectable), the sample will send for Intra-Cytoplasmic Sperm Injection (ICSI). Intervention 2: Control group: This study is a single arm clinical trial that has no control group.