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IRCT20180714040460N7 IRCT 2021-03-03 Islamic Azad University Evaluation of carcinogenicity of chlorhexidine mouthwash on oral mucosa Investigation of the relationship between chlorhexidine mouthwash and nuclear changes in buccal mucosa in people with gingivitis 2021-04-04 anticipated 50 Complete https://irct.ir/trial/54677 interventional Randomization: N/A, Blinding: Not blinded, Placebo: Not used, Assignment: Single, Purpose: Prevention. 2-3 Carcinogenic effect of chlorhexidine mouthwash. Intervention group: To evaluate the toxicity of chlorhexidine mouthwash and to diagnose its carcinogenicity, chlorhexidine mouthwash 0/2٪ 250 ml has been prepared by Najo Company and is supposed to refer to 50 patients of who refer to the periodontology department of Azad University of Tehran . Phase one should be treated by rinsing your mouth thoroughly with water before scaling, and a layer of cells is removed from the surface of the buccal mucosa of the mouth with a wet swab and applied to a clean, sterile slide. The slide is placed, then we fix it with spray fix, and then the mouthwash is rotated twice a day and night after brushing, each time 10 ml of solution,by time 30 seconds ,turned in mouth and Then emptied. The second session of treatment, which is one week later, will take the second sample . both samples after the steps of Pap smear and fixation and nucleus staining and dehydration, we will count nuclear changes of micronucleus, karyorexis, karyolysis and broken eggs with light microscopy.then we can judge the result.. Yes - There is a plan to make this available What will be shared: Investigation of the relationship between chlorhexidine mouthwash and nuclear changes Buccal mucosa in people with gingivitis When: 6 mounths after printing the results To whom: All people who needs data Conditions: The analysis that do not damage the outcome of our data analysis are allowed Where to obtain: Faculty of Dentistry, Islamic Azad University of Iran How to obtain: Refer to Tehran Azad Dental School and apply there and have access to the data for a maximum of 2 weeks Comments:
public Nima Nadaf Pour
No9,9th Neyestan Alley,Pasdaran Street,Tehran,Iran
Tehran Iran (Islamic Republic of) 1946853314 +98 21 2256 4571 N_nadaf@dentaliau.ac.ir Islamic Azad University scientific Nima Nadaf Pour
No9,9th Neyestan Alley,Pasdaran street,Tehran,Iran
Tehran Iran (Islamic Republic of) 1946853314 +98 21 2256 4571 N_nadaf@dentaliau.ac.ir Islamic Azad University Iran (Islamic Republic of) Be in the treatment of phase one gingivitis be between 20-30 years old Have gingivitis 30 years 60 years Both The smoking group includes samples with a history of consumption of more than 5 years and above 20 cigarettes per day that are considered Heavy Smoker criteria for taking certain drugs (such as antiretrovirals, doses containing statins, ..) Drug addiction engaging in high-risk occupations such as the paint industry, etc recent viral illness Undergoing radiotherapy or Less than one year history for radiotherapy Prevention Intervention group: To evaluate the toxicity of chlorhexidine mouthwash and to diagnose its carcinogenicity, chlorhexidine mouthwash 0/2٪ 250 ml has been prepared by Najo Company and is supposed to refer to 50 patients of who refer to the periodontology department of Azad University of Tehran . Phase one should be treated by rinsing your mouth thoroughly with water before scaling, and a layer of cells is removed from the surface of the buccal mucosa of the mouth with a wet swab and applied to a clean, sterile slide. The slide is placed, then we fix it with spray fix, and then the mouthwash is rotated twice a day and night after brushing, each time 10 ml of solution,by time 30 seconds ,turned in mouth and Then emptied. The second session of treatment, which is one week later, will take the second sample . both samples after the steps of Pap smear and fixation and nucleus staining and dehydration, we will count nuclear changes of micronucleus, karyorexis, karyolysis and broken eggs with light microscopy.then we can judge the result. Micronucleus. Timepoint: Before scaling and one week after using chlorhexidine mouthwash. Method of measurement: Cell count with light microscope with 400 magnification. Karyorrehxis. Timepoint: Before scaling and one week after using chlorhexidine mouthwash. Method of measurement: count with light microscope with 400 magnification. Karyolysis. Timepoint: Before scaling and one week after using chlorhexidine mouthwash. Method of measurement: count with light microscope with 400 magnification. Broken egg. Timepoint: Before scaling and one week after using chlorhexidine mouthwash. Method of measurement: count with light microscope with 400 magnification. Repair index. Timepoint: It is calculated after counting nuclear changes. Method of measurement: Mean karyolysis and karyorexia divided by the mean of micronucleus and Broken ag. Islamic Azad University of Tehran Approved 2019-06-01 Faculty of Dentistry, Islamic Azad University, Tehran Branch No. 9, Neystan Alley, Pasdaran St., Tehran, Iran Tehran Tehran Iran (Islamic Republic of)