IRCT201104166206N1 IRCT 2011-04-26 Vice chancellor for research of Tehran University of Medical Sciences The effect of breastfeeding on pain relief in newborns during vaccination The effect of breastfeeding on pain relief in full-term neonates during B vaccination 2007-11-12 anticipated 130 Complete https://irct.ir/trial/6639 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Prevention. N/A pain during hepatitis vaccination. Intervention 1: In the intervention group: Participating neonates and their mothers were taken to a quiet nursery room for immunization. The study did not start until the infant was observed to be sucking at the breast. Effective sucking movements were defined as movements in which the sucking sufficiently moved the temporomandibular joints to allow the infant to obtain maternal milk and eventually swallow it. This usually required 30-60 seconds to achieve. None of the infants had been breastfed for at least 30 minutes before the study procedures commenced. In experimental group, feeding was begun two minutes before, during, and after hepatitis B vaccination (n=65). At the end of the second minute of breastfeeding, while the infants were still sucking, the immunization injections were performed. Intervention 2: In the control group: neonates were not breastfed but held by the mother for the same length of time at the experimental group. In the controls experienced the same procedure while were held in mothers arms but not fed.