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IRCT20221030056345N1 IRCT 2023-07-16 Ilam University of Medical Sciences Evaluation the effect of distilled water, chlorhexidine mouthwash and ethanolic extract of Peganum Harmala plant on the count of Streptococcus mutans settled on removable orthodontic appliances Evaluation the effect of distilled water, chlorhexidine mouthwash and ethanolic extract of Peganum Harmala plant on the count of Streptococcus mutans settled on removable orthodontic appliances 2023-07-06 anticipated 30 Complete https://irct.ir/trial/70718 interventional Randomization: Not randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Single, Purpose: Prevention. 1-2 the count of Streptococcus mutans settled on removable orthodontic appliances. Intervention 1: Control: Distilled water-ph=6.5-7.5-TDS=0.00ppm-CL=0.00-once for 30 participants in a 10-day period-after removing the movable orthodontic plate from the mouth, the participant will immerse it in a container containing distilled water. and to put it back in the mouth, he will use tweezers to remove the mobile plaque from distilled water. After 10 days, the solution will be cultured - Sina Noor Company. Intervention 2: First Intervention: The first intervention group: Chlorhexidine 0.2% - mouthwash - every 100 ml containing: chlorhexidine gluconate 0.2 g + 10% ethanol - Najo company - the participant for 10 days after removing the removable orthodontic plate from the mouth, put it in a container containing Chlorhexidine mouthwash will be immersed and forceps will be used to remove the mobile plaque from the mouthwash to put it back in the mouth. After 10 days, the solution will be cultured. Intervention 3: Second Intervention: Ethanol extract of pecan plant-combination: 600 grams of pecan is prepared for the preparation of herbal mouthwash. After washing and drying, the fruit and seeds of the plant will be separated and ground with a grinder. The resulting powder is poured into a container and Its surface is completely covered with 96% ethanol and it remains in this state for 48 hours. Then the resulting mixture is placed in the oven. After 72 hours, when the solution remains in the oven, the solution is removed from the machine and passed through filter paper. Then the resulting solution is placed in the rotary device little by little and several times. And in this way, the alcohol is separated from the solution and a liquid is obtained, which after drying will be the same pecan plant extract that is used to prepare Different concentrations will be dissolved in normal saline serum.-The concentration used in the study will be lower than MTT (the highest value obtained from MIC determination) - the participant will immerse the movable orthodontic plate in a container containing pecan plant mouthwash for 10 days after removing it from the mouth. Placing it back in the mouth will use forceps to remove the moving plaque from the solution. After 10 days, the solution will be cultured. Yes - There is a plan to make this available What will be shared: Without mentioning the name of the participants, the amount of MIC and MBC of the plant extract and cfu obtained from the three materials used will be shared. When: The access period will start 1 year after the results are published To whom: The data will be accessible to researchers working in academic and scientific institutions and students interested in research Conditions: Data access is allowed if researchers use them to increase awareness and interest in conducting further studies Where to obtain: Email address: doctorfj1999@yahoo.com How to obtain: Applicants should include researchers working in academic and scientific institutions or students who, after sending the documents to the email address doctorfj1999@yahoo.com and mentioning the purpose of the data study, the result will be sent to them via email within 2-3 months at most. becomes Comments:
public Fatemeh Jafarzadeh
Amirkabir
Ilam Iran (Islamic Republic of) 6931836116 +98 84 3385 3746 doctorfj99@gmail.com Ilam University of Medical Sciences scientific Fatemeh Jafarzadeh
Amirkabir
Ilam Iran (Islamic Republic of) 6931836116 +98 84 3385 3746 doctorfj99@gmail.com Ilam University of Medical Sciences Iran (Islamic Republic of) Consent of participants to participate in the study Retainer prescription by an orthodontic specialist Age 20-60 years 20 years 60 years Both Active periodontal disease History of using chlorhexidine mouthwash during the last two weeks N/A Treatment - Drugs N/A Control: Distilled water-ph=6.5-7.5-TDS=0.00ppm-CL=0.00-once for 30 participants in a 10-day period-after removing the movable orthodontic plate from the mouth, the participant will immerse it in a container containing distilled water. and to put it back in the mouth, he will use tweezers to remove the mobile plaque from distilled water. After 10 days, the solution will be cultured - Sina Noor Company First Intervention: The first intervention group: Chlorhexidine 0.2% - mouthwash - every 100 ml containing: chlorhexidine gluconate 0.2 g + 10% ethanol - Najo company - the participant for 10 days after removing the removable orthodontic plate from the mouth, put it in a container containing Chlorhexidine mouthwash will be immersed and forceps will be used to remove the mobile plaque from the mouthwash to put it back in the mouth. After 10 days, the solution will be cultured. Second Intervention: Ethanol extract of pecan plant-combination: 600 grams of pecan is prepared for the preparation of herbal mouthwash. After washing and drying, the fruit and seeds of the plant will be separated and ground with a grinder. The resulting powder is poured into a container and Its surface is completely covered with 96% ethanol and it remains in this state for 48 hours. Then the resulting mixture is placed in the oven. After 72 hours, when the solution remains in the oven, the solution is removed from the machine and passed through filter paper. Then the resulting solution is placed in the rotary device little by little and several times. And in this way, the alcohol is separated from the solution and a liquid is obtained, which after drying will be the same pecan plant extract that is used to prepare Different concentrations will be dissolved in normal saline serum.-The concentration used in the study will be lower than MTT (the highest value obtained from MIC determination) - the participant will immerse the movable orthodontic plate in a container containing pecan plant mouthwash for 10 days after removing it from the mouth. Placing it back in the mouth will use forceps to remove the moving plaque from the solution. After 10 days, the solution will be cultured. Count of Streptococcus mutans settled on removable orthodontic appliances. Timepoint: 10 days after the intervention. Method of measurement: Through microbial culture and colony counting. ilam university of medical sciences Approved 2023-06-17 Research ethics committees of ilam university of medical sciences Pardis of Ilam University of Medical Sciences ,Paghoohesh Blvd,Banganjab,Ilam Ilam Ilam Iran (Islamic Republic of)