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IRCT20251202068190N1 IRCT 2026-04-11 University of Guilan The effect of resistance exercise with and without blood flow restriction during different phases of the menstrual cycle in trained women Hormonal, Lactate, and Cardiac Autonomic Responses to Resistance Exercise with and Without Blood Flow Restriction at Menstrual Cycle Phases in Trained Women 2025-06-17 anticipated 12 Complete https://irct.ir/trial/88341 interventional Randomization: Not randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Crossover, Purpose: Diagnostic. N/A Menstrual cycle status. Intervention 1: Control group: Resistance exercise without Blood Flow Restriction; Participants will perform traditional resistance exercise at an intensity of 70% of one-repetition maximum (1RM). The training protocol will include of two exercises, leg press and leg extension, each will be performed for 4 sets of 10 repetitions. Rest intervals will consist of 1 minute between sets and 2-3 minutes between two exercises. The intervention will be conducted over two consecutive menstrual cycles, such that one session will be performed during the follicular phase and one session during the luteal phase. Intervention 2: Intervention group: Resistance exercise with Blood Flow Restriction (BFR); Participants in this group will perform resistance exercises (leg press and leg extension) at an intensity of 30% of 1RM. BFR will be applied using a cuff placed at the proximal portion of the lower limb, with a pressure set at 60% of Limb Occlusion Pressure (LOP). The training protocol will consist of 4 sets (30-15-15-15 repetition), with one minute of rest between sets and 2-3 minutes between two exercises. The intervention will be conducted over two consecutive menstrual cycles, such that one session will be performed during the follicular phase and one session during the luteal phase. Yes - There is a plan to make this available What will be shared: This document includes a complete description of the study design, training protocol, intervention schedule, measurement methods for all variables, and study procedures, prepared to enhance transparency and reproducibility of the results. When: After publication of the study results To whom: Researchers and the academic staff Conditions: The data are available solely for research purposes, subject to confidentiality requirements and approval by the principal investigator. Where to obtain: Through the principal investigator. How to obtain: Email of the principal investigator Comments:
public Zahra Aftabi Talami
University of Guilan, 5th Kilometer of Rasht-Qazvin road, Rasht, Guilan province, Iran
Rasht Iran (Islamic Republic of) 4199613776 +98 13 3369 0685 zahraaftabi.t@gmail.com University of Guilan scientific Javad Mehrabani
University of Guilan, 5th Kilometer of Rasht-Qazvin road, Rasht, Guilan province, Iran
Rasht Iran (Islamic Republic of) 4199613776 +98 13 3369 0685 Mehrabanij@guilan.ac.ir University of Guilan Iran (Islamic Republic of) Trained women with at least six months of regular resistance training experience Regular menstrual cycles (28–32 days) Range of age between 20 to 30 years 20 years 30 years Female Taking hormonal medications or oral contraceptives during the past six months Consumption of nutritional supplements or caffeine at least 48 hours prior to testing The history of cardiovascular, metabolic, endocrine, or neurological diseases Diagnosis Diagnosis Control group: Resistance exercise without Blood Flow Restriction; Participants will perform traditional resistance exercise at an intensity of 70% of one-repetition maximum (1RM). The training protocol will include of two exercises, leg press and leg extension, each will be performed for 4 sets of 10 repetitions. Rest intervals will consist of 1 minute between sets and 2-3 minutes between two exercises. The intervention will be conducted over two consecutive menstrual cycles, such that one session will be performed during the follicular phase and one session during the luteal phase. Intervention group: Resistance exercise with Blood Flow Restriction (BFR); Participants in this group will perform resistance exercises (leg press and leg extension) at an intensity of 30% of 1RM. BFR will be applied using a cuff placed at the proximal portion of the lower limb, with a pressure set at 60% of Limb Occlusion Pressure (LOP). The training protocol will consist of 4 sets (30-15-15-15 repetition), with one minute of rest between sets and 2-3 minutes between two exercises. The intervention will be conducted over two consecutive menstrual cycles, such that one session will be performed during the follicular phase and one session during the luteal phase. Growth hormone. Timepoint: Pre-test (before the exercise session) and post-test (after the test/end of the exercise session). Method of measurement: Serum growth hormone concentration will be measured through blood sampling and laboratory assessment using the Enzyme-Linked Immunosorbent Assay (ELISA) method. Cortisol. Timepoint: Pre-test (before the exercise session) and post-test (after the test/end of the exercise session). Method of measurement: Serum cortisol concentration will be measured using blood sampling and laboratory analysis by the enzyme-linked immunosorbent assay (Enzyme-Linked Immunosorbent Assay). Blood Lactate. Timepoint: Pre-test (before the exercise session) and post-test (after the test/end of the exercise session). Method of measurement: Plasma lactate level will be measured using blood sampling and laboratory analysis by a standard enzymatic method. Heart Rate Variability. Timepoint: Heart rate variability will be recorded and analyzed in 5-minute time windows during the pre-test period and immediately after the test. Method of measurement: Heart rate variability will be measured and analyzed using a Polar H10 heart rate sensor. Blood Pressure. Timepoint: Pre-test (before the exercise session) and post-test up to 60 minutes after the test. Method of measurement: By using Sphygmomanometer. Rating of Perceived Exertion (RPE). Timepoint: Immediately after completion of each set of the exercise. Method of measurement: By using the Borg rating of perceived exertion scale. University of Guilan Approved 2025-04-14 Ethics committee of University of Guilan University of Guilan, 5th Kilometer of Rasht-Qazvin road, Rasht, Guilan province, Iran Rasht Guilan Iran (Islamic Republic of)