IRCT201402208384N3 IRCT 2014-08-01 Vice Chancellor for Research, Mashhad University of Medical Science Comparison of general and spinal anesthesia on the body's defense systems in diabetic patients Comparison of the effect of Spinal and General Anesthesia on Serum Lipid Peroxides and Total Antioxidant Capacity in Diabetic Patients with Lower Limb Amputation Surgery 2012-11-30 anticipated 63 Complete https://irct.ir/trial/8862 interventional Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Diagnostic. 2-3 Diabetes. Intervention 1: Intervention group 1 : In group 1 with general anesthesia (n = 20), pretreatment with midazolam 04/0 mg kg, fentanyl 3 micrograms per kilogram, and the induction of anesthesia with propofol 2 mg kg, were used. Intervention 2: Intervention group 2 : For patients in SA group, 3 ml Bupivacaine 0.5% was injected into the subarachnoid space of the spinal canal between the third and fourth lumbar space or between the fifth lumbar and first sacral space of the spinal column. Intervention 3: Control group: 23 healthy volunteers (age and gender-matched) were recruited to have a control group for a better interpretation of laboratory data in our patients.