]>
IRCT20250517065774N4 IRCT 2026-09-13 Islamic Azad University Effects of exercise training and sports drink on cellular markers in soccer players Evaluation the role of eight weeks of SAQ and sprint interval training on mitochondrial indices and cell-free DNA concentration in soccer players along with consumption of sports drinks. 2026-09-22 anticipated 56 Complete https://irct.ir/trial/92157 interventional Randomization: Randomized, Blinding: Double blinded, Placebo: Used, Assignment: Parallel, Purpose: Basic scienece, Randomization description: To use the random number table, the researcher will first determine the table's reading direction. Then the numbers will be considered for different groups. For example, numbers 14-00 for group A, numbers 15-29 for intervention B, numbers 44-30 for group C, and 45-59 for group D. Then, we will place our hands on one of the numbers and move in one of the predetermined directions. The numbers will be recorded and assigned to four groups: SAQ group (agility/speed drills); SIT (intermittent sprints) with stretching recovery and Spirulina drink consumption; SAQ group (agility/speed drills) with stretching recovery and Spirulina drink consumption; and SIT (intermittent sprints) with stretching recovery and Spirulina drink consumption. To conceal the random allocation, the coded-box method with random sequences will be used. This method uses several boxes numbered in a random sequence. It will be ensured that the boxes are sufficiently sealed, identical in appearance, and equal in weight, Blinding description: In this study, the analyst, statistician, specialist assistant, coach and physiologist were unaware of the entire study process. The blinding process included allocation concealment through sealed envelopes and spirulina drink consumption, standardized exercise training protocols, and outcome assessment by an independent blinded team with automated digital devices coupled with an emergency controlled disclosure mechanism designed for emergencies. In order to blind the consumption of spirulina drink, all the participants in the recovery stage are given a simple drink in dark bottles so that the color of the drink is not clear. 2-3 Mitochondrial indices and cell free DNA concentration and cell death. Intervention 1: Intervention group 1: SAQ group (Agility/Speed ​​Drills): Perform SAQ (Speed, Agility, Quickness) exercises and include a 10-minute warm-up with light jogging and dynamic movements, 10-30 meter sprint speed exercises, agility and direction changes, and quickness and reaction exercises. The intensity of the exercise is set in the range of 85-95% of maximum heart rate and the duration of each session is 30-40 minutes. Intervention 2: Intervention group 2: SIT group: The SIT protocol consisted of four sets of maximal-intensity sprints interspersed with short recovery periods between sets and longer recovery periods between sets. Intervention 3: Intervention group 3: SAQ group (agility/speed drills) + recovery with stretching and consumption of 500 mg of spirulina drink. Intervention 4: Intervention group 4: SIT group + recovery with stretching and consumption of 500 mg of Spirulina drink. Undecided - It is not yet known if there will be a plan to make this available Justification or reason for indecision in sharing IPD is Undecided - It is not yet known if there will be a plan to make this available
public Navid Abedpoor
Daneshgah Boulevard, Arghavanieh, Jay Sharqi Street, Isfahan
Isfahan Iran (Islamic Republic of) 39998-81551 +98 31 3535 2900 abedpoor.navid@yahoo.com Islamic Azad University scientific Navid Abedpoor
Islamic Azad Univesity Of Isfahan (Khorasgan) Branch -Daneshgah blvd, Arghavanieh, East Jey St, Isfahan, Iran
Isfahan Iran (Islamic Republic of) 39998-81551 +98 31 3535 2900 abedpoor.navid@yahoo.com Islamic Azad University Iran (Islamic Republic of) Male Age of 14-15 years old No musculoskeletal abnormalities With informed consent from the player's parents. No supplements/medications (except a standard multivitamin) Completion of the PAR-Q questionnaire and normal pre-exercise ECG 14 years 15 years Male History of known cardiovascular or pulmonary diseases Presence of acute or chronic injuries in limbs over the past 6 months Smoking or alcohol consumption Having metabolic disorders such as diabetes Other Other Other Other Intervention group 1: SAQ group (Agility/Speed ​​Drills): Perform SAQ (Speed, Agility, Quickness) exercises and include a 10-minute warm-up with light jogging and dynamic movements, 10-30 meter sprint speed exercises, agility and direction changes, and quickness and reaction exercises. The intensity of the exercise is set in the range of 85-95% of maximum heart rate and the duration of each session is 30-40 minutes. Intervention group 2: SIT group: The SIT protocol consisted of four sets of maximal-intensity sprints interspersed with short recovery periods between sets and longer recovery periods between sets. Intervention group 3: SAQ group (agility/speed drills) + recovery with stretching and consumption of 500 mg of spirulina drink. Intervention group 4: SIT group + recovery with stretching and consumption of 500 mg of Spirulina drink. Gene expression of PGC-1a in peripheral blood mononuclear cells in young soccer players. Timepoint: At the beginning and end of the study after 8 weeks. Method of measurement: Real-time qPCR. Citrate synthetase concentration. Timepoint: At the beginning and end of the study after 8 weeks. Method of measurement: Elisa Kit. Serum cfDNA concentration. Timepoint: At the beginning and end of the study after 8 weeks. Method of measurement: cfDNA concentration assay kit. Gene expression of COX in peripheral blood mononuclear cells in young soccer players. Timepoint: At the beginning and end of the study after 8 weeks. Method of measurement: Real-time qPCR. Gene expression of MFN2 in peripheral blood mononuclear cells in young soccer players. Timepoint: At the beginning and end of the study after 8 weeks. Method of measurement: Real-time qPCR. Aerobic power. Timepoint: At the beginning and end of 8 weeks of study. Method of measurement: Yo-Yo Aerobic Endurance Test Level 1. Anaerobic Resistance. Timepoint: At the beginning and end of 8 weeks of study. Method of measurement: RAST field test. Dribbling speed skills in Soccer. Timepoint: At the beginning and end of 8 weeks of study. Method of measurement: Ronaldo's dribbling speed test (Hulichi et al., 2014; Wood, 2024). Peak power and Average power. Timepoint: At the beginning and end of 8 weeks of study. Method of measurement: Reciprocal Sargent test using the formula: Peak power (W) = 78.5 x VJ (cm) + 60.6 x mass (kg) -15.3 x height (cm) -1308 and the formula: Average power (W) = 41.4 x VJ (cm) + 31.2 x mass (kg) -13.9 x height (cm) + 431. Islamic Azad University Approved 2026-07-28 Ethics Committee on Research Islamic Azad University - Isfahan Branch (Khorasgan) Daneshgah Boulevard, Arghavanieh, East Gey Street, Isfahan Isfahan Isfehan Iran (Islamic Republic of)