This study is a randomized, double-blind controlled clinical trial (patient and pain assessor were blinded, the other was known materials and did the intervention). Inclusion criteria were patients who attended in Dental School of Shahid sadoughi University of Medical Sciences, Yazd, Iran, sensitive teeth with signs of abrasion, erosion or cervical dentin’sexposure. Exclusion criteria were teeth with evidence of pulpitis, caries lesions, defective restorations, enamel’s crack, active periodontal disease, decay or deterioration in the cervical area that needs to class V fillings, cracked or endodontically treated teeth; teeth with large restoration; fixed prosthesisor as abutmentof removable prosthesis; daily intake of anti-inflammatory drugs, analgesics, tooth desensitizer; patients with surgical periodontal ororthodontic treatment in the last 3 months; suppressed immune systems; pregnant and lactating patients; systemic conditions is caused or predictive factor for DH or too much acidic diet. After selecting appropriate patients, informed consent was filled by patients and they were enrolled. Cluster randomization was done with inequal clusters (the number of sensitive teeth per person). From by random numbers table, patients divided into three groups. Depending on the number sensitive teeth per person sample size had been completed in each groups.
The adjacent teeth were isolated with cotton rolls and then air blast was vertically applied by 2cm in 3 seconds. Tactile stimulation was done by swipping and vertically explorer’s pressure on cervical area of tooth which was gradually increased.
The sensitivity of the patients was measured by a VAS scale. After baseline assessment, 3 desensitizing agents including propolis (A), HA (B) and placebo (C) were applied and Up to 5 minutes remained isolated. It is also recommended not washing their mouth for next 30 minutes. In the begining of study, the same soft toothbrus and toothpaste were given to them to use twice a day (morning and evening before bedtime). Pain score were measured 2 and 4 weeks after the intervention.