Comparison of the antibacterial effectiveness of thymes and chamomile extract and chlorhexidine on the salivary Streptococcus mutans of preschool childrens with early childhood caries by the quantitative Real Time PCR

Comparison of the antibacterial effectiveness of thymes and chamomile extract and chlorhexidine on the salivary Streptococcus mutans of preschool childrens with early childhood caries by the quantitative Real Time PCR

In this clinical trial study, there will be 90 preschool children aged 4-6 who will be matched from demographic data. Children are randomly divided into 3 groups of30 person and each participant assigned a code.

ّFirst the aims of the study explain to the parents, then written informed consent is taken. A questionnaire including the demographic data of the child (age, sex, history of drug use, history of systemic disease, etc.) is also completed.Then, the methods of oral hygiene and brushing explain to all parents and wanted to brush the teeth of their children without toothpaste for 2 days. also it is emphasized that during the study, the children do not use gums containing Xylitol and any type of mouthwashes containing chlorhexidine and fluoride. Upon completion of the two-day period, Which is considered as a washout period, children are randomly divided into 3 groups of 30 person. then from the participant children will take the sample of saliva and transfer to laboratory for determining the amount of Streptococcus mutans.

The inclusion criteria including preschool children aged 4 to 6 years that have at least dmft 4 and 3 dental caries in their mouth.exclusion criteria including preschool children with sysytemic disease , during the past month take antibiotic and topical flouride and have orthodontic appliance, who take gums containing Xylitol and mouthwash or gel containing flouride at least 3 weeks before study, who have soft tissue lesion in their mouth and have active and severe periodontal disease.

Intervention group 1: thymes extract 5% , 30 minutes and 1 week after first salivary sampling from 4 to 6 year old children, 1 cc gel measured with a syringe and with a cotton swab is applied to all teeth of the child. Intervention group 2: chamomile extract 5% , 30 minutes and 1 week after first salivary sampling from 4 to 6 year old children, 1 cc gel measured with a syringe and with a cotton swab is applied to all teeth of the child. Control group1: cholorhexidine 0.02 % , 30 minutes and 1 week after first salivary sampling from 4 to 6 year old children, 1 cc gel measured with a syringe and with a cotton swab is applied to all teeth of the child.

Streptococcus mutans,

Simple randomization or unrestricted method will be used and randomization unit is Individual .we will use from Roll of a die from the methods of simple randomization because there are 3 groups in this study that actually the tool of randomization is dice. So the numbers 1 and 2 are for the first group (chlorhexidine) 3 and 4 for the second group ( thymes) and 5 and 6 for the third (chamomile). The next step Allocation concealment will be done, In such a way that the allocated group is not known before the assignment of the individual. To achieve this goal, a non-transparent, sealed, random sequence envelope is used . In this way, the statistical specialist will provide 90 envelopes and each of the randomly generated links will be recorded on a card and the cards are arranged in the envelopes . In order to maintain a random sequence, the numbering of envelopes on the outer surface is done in the same order. Finally, the envelops are enclosed and placed in the box, respectively. At the time of the implementation of the study, according to the order of entry of eligible participants, one of the envelopes is opened respectively and will determine the group assigned to that participant. In this study, the assistant of Pediatric Dentistry examines the participants in terms of inclusion and exclusion criteria. Randomization methods are done by an statistical specialist and performance of the study is done by a trained dental student.

Because all the materials are in the same consistency gel, in a uniform shape and color, considering the informed consent, participants are unaware of material that what materials is used for. Outcome evaluator, who performs PCR, is also unaware of the groups.

Because the study data do not have any other scientific application.

After this study, data and statistical analysis data are available to all.

After completing the research plan for 5 years of information dissemination

All Researchers and Faculty Members

In order to conduct new researches

Department of Research and Technology Faculty

Submitting a request to the person responsible for collecting documentation of research projects at the Department of Research and and technology Faculty