View older revisions Content changed at 2022-02-09, 1400/11/20

Protocol summary

Study aim
This clinical trial aims to compare the effects of black seed cream with tretinoin cream in acne vulgaris
Design
This study is a double-blind randomized clinical trial.
Settings and conduct
This clinical trial will be conducted in The Center for Research and Training in Skin Diseases and Leprosy, and Razi hospital of Tehran University of medical sciences. Participants and healthcare providers will be blind.
Participants/Inclusion and exclusion criteria
Inclusion criteria: individuals aged between 18-50 years old (regardless of gender) diagnosed with mild to moderate facial acne vulgaris (GAGS score ranging from 1 to 30); discontinued any topical or systemic treatments for acne at least 3 months prior to the intervention. Exclusion criteria: women with hirsutism or hormonal problems, pregnancy, breastfeeding, sensitivity to tretinoin or balck seed, and candidates for isotretinoin treatment.
Intervention groups
The number of 20 patients participating in this study are considered as 40 half faces, and are allocated into 2 equal groups receiving either tretinoin 0.05% or black seed creams for 8 weeks. Through the treatment period and in the one-month follow-up period, the patients will use identical facial cleansers and sunscreens.
Main outcome variables
Acne lesion counts; reduction of GAGS score; the adverse reactions of the intervention medications.

General information

Reason for update
Firstly, due to COVID pandemy, sample size and treatment duration were decreased, additionally, completing the study took longer time than expected. Secondly, in order to assess the acne lesions more detailedly, the study took place in The Center of Research and Training in Skin Diseases and Leprosy, in which its equipments (visiopore device, tewameter, corneometer, pHmeter, and mexameter probes) were employed.
Acronym
IRCT registration information
IRCT registration number: IRCT20191021045173N1
Registration date: 2020-04-03, 1399/01/15
Registration timing: prospective

Last update: 2022-02-09, 1400/11/20
Update count: 1
Registration date
2020-04-03, 1399/01/15
Registrant information
Name
Sarvenaz Zandkarimi
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 21 2264 0051
Email address
sarvenazkarimi96@gmail.com
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2020-04-08, 1399/01/20
Expected recruitment end date
2021-03-20, 1399/12/30
Actual recruitment start date
2020-11-01, 1399/08/11
Actual recruitment end date
2021-10-01, 1400/07/09
Trial completion date
2022-01-05, 1400/10/15
Scientific title
Efficacy and safety of Nigella sativa cream (20%) vs. tretinoin cream (0.05%) for acne vulgaris: a randomized right/left double-blind comparative clinical trial
Public title
Black seed vs. Tretinoin in acne vulgaris
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Age between 18-50 years Clinical diagnosis of mild to moderate facial acne (GAGS score ranging from 1 to 30) made by a dermatologist Discontinuation of any topical or systemic treatment for acne from at least 3 months prior to participation
Exclusion criteria:
Pregnancy or lactation, Women with hirsutism, menstrual disorders and hormonal problems, Severe grade of acne, Candidate for isotretinoin treatment, Known allergy to black seed or tretinoin, Subjects who are non-cooperative or unsatisfied with the treatment
Age
From 18 years old to 50 years old
Gender
Both
Phase
2
Groups that have been masked
  • Participant
  • Care provider
  • Investigator
  • Outcome assessor
Sample size
Target sample size: 40
More than 1 sample in each individual
Number of samples in each individual: 2
One half of the face will receive tretinoin cream while in the other half, black seed cream will be applied.
Actual sample size reached: 20
More than 1 sample in each individual
Actual sample size in each individual: 2
One half of the face received tretinoin cream, and the other half, back seed cream
Randomization (investigator's opinion)
Randomized
Randomization description
Treatment tubes were randomly labeled by a third party not involved in the study from 1 to 40 so that the tubes with odd numbers contained NS 20% cream and the ones with even numbers contained tretinoin 0.05% cream. The 20 patients were considered as 40 half faces and were randomly allocated into two comparison groups. Simple randomization procedure with an 1 : 1 allocation ratio was performed as following: subjects with odd admission numbers were supposed as ‘right half-face: odd-numbered tube’ (group I) and the ones with even admission numbers were assumed as ‘left half-face: odd-numbered tube’ (group II).  In each case, the reverse half-face was given an even-numbered tubes.
Blinding (investigator's opinion)
Double blinded
Blinding description
Treatment tubes were randomly labeled by a third party not involved in the study from 1 to 40 so that the tubes with odd numbers contained NS 20% cream and the ones with even numbers contained tretinoin 0.05% cream.
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Tehran Islamic Azad University of Medical Sciences
Street address
Faculty of pharmacy, Islamic Azad University of Medical Sciences, Yasaman Alley, Yakhchal Street, District 1, Tehran.
City
Tehran
Province
Tehran
Postal code
1941933111
Approval date
2019-12-04, 1398/09/13
Ethics committee reference number
IR.IAU.TMU.REC.1398.171

Health conditions studied

1

Description of health condition studied
Acne vulgaris
ICD-10 code
L70.0
ICD-10 code description
Acne vulgaris

Primary outcomes

1

Description
Acne lesion counts
Timepoint
Baseline (before intervention) and 4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
Independent assessment of lesion counts: comedones, papules/pustules

2

Description
Reduction of Global Acne Grading System (GAGS) score
Timepoint
Baseline (before intervention) and 4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
Rating of acne according to Global Acne Grading System (GAGS) by two dermatologists

3

Description
Patients satisfaction
Timepoint
4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
Patient subjective satisfaction score ranging from 1 to 100

4

Description
Adverse reactions of the study medications
Timepoint
4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
Patients reports and clinical assessments by two dermatologists

5

Description
Photographic assessment
Timepoint
Baseline (before intervention) and 4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
Digital camera

6

Description
Visiopore parameters (quantity, size, and value)
Timepoint
Baseline (before intervention) and 4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
Visiopore device

7

Description
skin hydration
Timepoint
Baseline (before intervention) and 4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
Corneometer probe

8

Description
skin erythema
Timepoint
Baseline (before intervention) and 4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
mexameter probe

9

Description
skin melanin
Timepoint
Baseline (before intervention) and 4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
mexameter probe

10

Description
Transepidermal water loss
Timepoint
Baseline (before intervention) and 4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
Tewameter probe

11

Description
Skin pH
Timepoint
Baseline (before intervention) and 4 , 8 weeks after intervention, and one month after discontinuation of treatments (the 12th week after baseline)
Method of measurement
pHmeter probe

Secondary outcomes

empty

Intervention groups

1

Description
20 patients are considered as 40 half faces ,and 2 comparison groups are designed as 20 right and 20 left half faces in order to omit the interindividual differences and compare each participant responses to the medications with her/himself. Patients will be individually instructed to apply a thin layer of the given topical medications which are labelled as "left" and "right" on the determined side of the clean facial skin for 8 weeks as twice daily administration. Through the treatment period and the one-month follow-up period, the patients will use identical facial cleansers and sunscreens.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Center for Research & Training in Skin Diseases & Leprosy
Full name of responsible person
Dr. Alireza Firooz
Street address
The Center for Research and Training in Skin Diseases and Leprosy, No. 415, Naderi St., Taleghani Ave.
City
Tehran
Province
Tehran
Postal code
1416613675
Phone
+98 21 8896 0880
Email
dermalab@tums.ac.ir

2

Recruitment center
Name of recruitment center
Razi Hospital
Full name of responsible person
Dr. Maryam Daneshpazhooh
Street address
Razi Hospital, Vahdat Eslami Square
City
Tehran
Province
Tehran
Postal code
1199663911
Phone
+98 21 5563 0223
Email
razihospital@sina.tums.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Islamic Azad University
Full name of responsible person
Dr. Farshad Hashemian
Street address
Department of Clinical Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, PO Box 19419, Yasaman St, Yakhchal Ave, Qolhak Ave, Shariati Ave, Tehran, Iran
City
Tehran
Province
Tehran
Postal code
19419
Phone
+98 21 2260 9043
Email
sarvenazkarimi96@gmail.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Islamic Azad University
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Dr. Mehdi Rajabi
Position
Assistant professor
Latest degree
Specialist
Other areas of specialty/work
Clinical pharmacy
Street address
Department of Clinical Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, PO Box 19419, Yasaman St, Yakhchal Ave, Qolhak Ave, Shariati Ave, Tehran, Iran
City
Tehran
Province
Tehran
Postal code
19419
Phone
+98 21 2264 0056
Email
mehdirj@aol.co.uk

Person responsible for scientific inquiries

Contact
Name of organization / entity
Iran University of Medical Sciences
Full name of responsible person
Dr. Zahra Azizian
Position
Dermatologist
Latest degree
Specialist
Other areas of specialty/work
Dermatology
Street address
Iran University of Medical Sciences, Shahid Hemmat Highway, Tehran, 1449614535, IRAN
City
Tehran
Province
Tehran
Postal code
۱۴۴۹۶۱۴۵۳۵
Phone
+98 21 2264 0056
Fax
Email
azizian.z@yahoo.com

Person responsible for updating data

Contact
Name of organization / entity
Islamic Azad University
Full name of responsible person
Dr. Sarvenaz Zandkarimi
Position
Pharm. D.
Latest degree
Ph.D.
Other areas of specialty/work
Dermatology
Street address
Department of Clinical Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, PO Box 19419, Yasaman St, Yakhchal Ave, Qolhak Ave, Shariati Ave, Tehran, Iran
City
Tehran
Province
Tehran
Postal code
19419
Phone
+98 21 2264 0056
Email
sarvenazkarimi96@gmail.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
Title and more details about the data/document
Only the data related to the measured outcomes will be published
When the data will become available and for how long
Availability starts after publication
To whom data/document is available
Available for people working in academic institutions
Under which criteria data/document could be used
Applicants aught to send a request email containing their aims and how the data will be used to the given gmail address
From where data/document is obtainable
contact: Sarvenazkarimi96@gmail.com
What processes are involved for a request to access data/document
After assessment of the applicant's request, if agreed, the data files will be sent in the shortest possible time .
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