Determining the effect of vitamin D on the expression of inflammatory NLRP3 genes and oxidative stress in Patients with type 2 diabetes

Measurement and comparison of the level of biochemical parameters, the level of vitamin D, the level of oxidative stress indicators, the level of expression of the Nlrp3 gene in the studied groups

Clinical trial with control group, double-blind, randomized, phase 3-2 on 68 patients. Block-chain with Excel software was used for randomization.

First and after 8 weeks of intervention, from each individual, 10 cc of intravenous blood will be taken by a sampler at the hospital (morning blood sample with 12 hours of fasting) and will be divided into CBC vials (containing K2EDTA ) and clots. The samples are immediately transferred to laboratory and serum is separated and stored at freezing (-20. C) and CBC vials are kept at refrigerator (2-6. C).To perform biochemical tests (LDL, HDL, vitamin D and oxides) on the serum sample of the clot tube, and to perform genetic testing and measurement of HbA1C, CBC tube is used (LDL, HDL) tests with kits and The enzymatic method, HbA1C will be measured by Immunoturbidimetry technique and vitamin D will be measured with low luminescence.And for genetic testing, PCR real time technique is used, The status of oxidative stress is assessed with TAC kits by FRAP, Thiol group and MDA by spectrophotometry.

Inclusion : The disease (diabetes) has been confirmed by a specialist. At least 5 years have passed since the beginning of their diabetes HbA1C is out of the normal range. Age range 50-65 years The desire to participate in the study Exclusion : Kidney and adrenal gland diseases, thyroid, liver,Systemic Take multivitamins,corticosteroids

Patients were randomly assigned to two groups (n = 34) to receive a dose of 50,000 units of vitamin D or placebo for 8 weeks each week.

HbA1C؛HDL؛ LDL ؛Nlrp3 ؛Oxidative stress status

Simple randomization, block

Individual participants are more likely to have vitamin D interventions Either Placebo was unaware, but they were well aware of the goals, methods, and beneficial consequences, and the potential dangers of studying, and the problems that could follow. Stop cooperating. And the doctor was aware of the participants' informed and free consent, and patients who were not at risk for drug intervention and people who were deficient in vitamin D were referred by a doctor, but the doctor did not know how the accident happened. Data collection officials and those assessing the outcome were unaware of the type and manner of intervention

Information about the main consequence or the like can be shared.

Start the access period from 2021 and after printing the results

It will be available for researchers working in academic and scientific institutions

In case of further study in this field and related studies

Birjand University of Medical Sciences, Central Library

It must be pursued through the university