View older revisions Content changed at 2023-02-27, 1401/12/08

Protocol summary

Study aim
Determination of antihypertensive effect of antioxidant enriched extract of Ziziphus jujuba fruit in patients with primary hypertension
Design
Two arm parallel groups randomized trial with blinded postoperative care and outcome assessment
Settings and conduct
Patients with primary hypertension (hypertension and stage 1) referred to the clinic of Sayad Shirazi Medical Center of Golestan Medical Sciences over 18 years of age, randomly, double-blind, placebo-controlled study will be included in the study.
Participants/Inclusion and exclusion criteria
Inclusion criteria: mmHg120 <blood pressure <mmHg 160 (Pre- and stage 1 hypertension) Age over 18, female or male Voluntary participation in this clinical study and submission of written consent Exclusion criteria: Other heart diseases Secondary blood pressure Serum potassium> 5.5 mmol / L or <3.5 mmol / L double Serum creatinine in the last 6 months Severe complications of diabetes or serious macrovascular events within 6 months (e.g., cerebral hemorrhage, cerebral infarction, or acute myocardial infarction) Recent infection in the last 4 weeks Primary or secondary kidney disease (e.g., IgA nephropathy, membranous nephropathy, or lupus nephritis) Any cancer and malignancy Severe mental disorder Pregnant or lactating women or women who are planning to become pregnant or women who are not using appropriate contraceptive methods Participate in other clinical trials Drug allergies or jujube allergies Use of other herbal medicines to control the symptoms of the present disease History of smoking, drugs Other conditions that the researchers considered inappropriate in this clinical study
Intervention groups
Control group: routine drug treatment + placebo for 12 weeks Intervention group: routine drug treatment + extract of Ziziphus jujuba fruit for 12 weeks
Main outcome variables
Blood pressure

General information

Reason for update
Change of sponsors of financial resources and delay in sampling time.
Acronym
IRCT registration information
IRCT registration number: IRCT20200506047325N1
Registration date: 2021-11-18, 1400/08/27
Registration timing: prospective

Last update: 2023-02-27, 1401/12/08
Update count: 1
Registration date
2021-11-18, 1400/08/27
Registrant information
Name
Ayesheh Enayati
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 17 3245 1434
Email address
enayati_phyto@yahoo.com
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2023-04-21, 1402/02/01
Expected recruitment end date
2024-04-20, 1403/02/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Investigation of antihypertensive effect of antioxidant enriched Ziziphus jujuba fruit extract in patients with primary hypertension: A randomized, double-blind, placebo controlled clinical trial
Public title
Evaluation of anti-hypertensive effect of jujube fruit
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
120 mmHg < blood pressure < mmHg 160 (Pre- and stage 1 hypertension) Age over 18, female or male Voluntary participation and written consent
Exclusion criteria:
Other heart diseases Secondary blood pressure Serum Potassium > 5.5 mmol/L or < 3.5 mmol/L double serum creatinine in the last 6 months Severe complications of diabetes or serious macrovascular events within 6 months (for example, cerebral hemorrhage, cerebral infarction, or acute myocardial infarction) Recent infection in the last 4 weeks Primary or secondary kidney disease (e.g., IgA nephropathy, membranous nephropathy, or lupus nephritis) Any cancer and malignancy Severe mental disorder Pregnant or lactating women or women who are planning to become pregnant or women who are not using appropriate contraceptive methods Drug allergies or jujube allergies Participate in other clinical trials Use of other herbal medicines to control the symptoms of the present disease History of smoking, drugs Other conditions that the researchers considered inappropriate in this clinical study
Age
From 18 years old to 70 years old
Gender
Female
Phase
N/A
Groups that have been masked
  • Participant
  • Outcome assessor
  • Data analyser
Sample size
Target sample size: 48
Randomization (investigator's opinion)
Randomized
Randomization description
The random assignment list will be computer generated with a 1:1 allocation, stratified by recruitment site, using random block size of four. Using concealed in sequentially numbered, sealed, opaque envelopes (SNOSE), participants will enter the blocks in such a way that an equal number of each assigned group. Allocation will be done by randomly selecting one of the arrangements and assigning the next part of the participants to the study groups according to the specified sequence. And kept by the hospital pharmacist of the center.
Blinding (investigator's opinion)
Double blinded
Blinding description
To maintain blindness in patients, the drug and placebo are the same in shape and appearance; Also, the evaluator is not aware of the type of drugs in the intervention groups, so that multi-digit numerical codes are written on the drug packages that only the main person in charge of the research is aware of.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Research Ethics Committees of Golestan University of Medical Sciences
Street address
Shastkola Road
City
Gorgan
Province
Golestan
Postal code
14395-477
Approval date
2021-10-31, 1400/08/09
Ethics committee reference number
IR.GOUMS.REC.1400.273

Health conditions studied

1

Description of health condition studied
Hypertension
ICD-10 code
I10
ICD-10 code description
Essential (primary) hypertension

Primary outcomes

1

Description
blood pressure
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Mercury sphygmomanometer

Secondary outcomes

1

Description
SCr
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Blood and urine tests

2

Description
eGFR
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Blood and urine tests

3

Description
Serum Albumin
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Blood and urine tests

4

Description
Serum Potassium
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Blood and urine tests

5

Description
EGC
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Electrocardiograph

6

Description
Liver enzymes
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Blood tests

7

Description
ACE2 enzyme
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Bloodtests

8

Description
Serum Nitric oxide
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Blood tests

9

Description
Lipid profiles
Timepoint
0: Start studying and receiving medication (in person),1: 2 weeks after receiving stage 1 medication (in person), 3: Week 8 End of stage 2 drug treatment course (in person), 4: Week 12 after receiving the drug stage 3 (face-to-face visit), 5: First follow-up - 2 weeks after receiving the drug (week 14) (telephone follow-up), 6: Second follow-up - 4 weeks after receiving the drug (week 16) (face-to-face visit)
Method of measurement
Blood tests

10

Description
diet
Timepoint
Before and after 12 weeks of intervention
Method of measurement
Diet with 24Recall checklist

Intervention groups

1

Description
Intervention group: Receiving routine drug treatment + enriched antioxidant extract of Ziziphus jujuba fruit for 12 weeks (3 months). Patients will be evaluated for symptoms at 14 weeks and 16 weeks after the end of the study.
Category
Treatment - Drugs

2

Description
Control group: Receiving routine drugs+ placebo for 12 weeks (3 months)
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Ischemic Disorders Research Center, Golestan University of Medical Sciences
Full name of responsible person
Dr. Ayesheh Enayati
Street address
Shastkola Road
City
Gorgan
Province
Golestan
Postal code
14395-477
Phone
+98 17 3245 1434
Fax
+98 17 3245 1434
Email
enayati_phyto@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Golestan University of Medical Sciences
Full name of responsible person
Dr. Ayesheh Enayati
Street address
Shastkola Road
City
Gorgan
Province
Golestan
Postal code
4934174515
Phone
+98 17 3245 1434
Email
enayati_phyto@yahoo.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Golestan University of Medical Sciences
Proportion provided by this source
50
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

2

Sponsor
Name of organization / entity
South Khorasan Academic center for education, culture and research
Full name of responsible person
Dr. Mahdi Ebrahimi
Street address
Birjand - Pasdaran St. - Quds St
City
Birjand
Province
South Khorasan
Postal code
466
Phone
+98 56 1222 3006
Email
Sec.khj@acecr.ac.ir
Web page address
http://jdkhj.ir/fa
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
South Khorasan Academic center for education, culture and research
Proportion provided by this source
33
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

3

Sponsor
Name of organization / entity
Food and Bio-Nanotech International Research Center (Fabiano)
Full name of responsible person
Elham Assadpour
Street address
Gorgan University of Agricultural Science and Natural Resources
City
gorgan
Province
Golestan
Postal code
49138-15739
Phone
+98 17 3225 1701
Email
smjafari@gau.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
No
Title of funding source
Food and Bio-Nanotech International Research Center (Fabiano)
Proportion provided by this source
33
Public or private sector
Private
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Industry

Person responsible for general inquiries

Contact
Name of organization / entity
Gorgan University of Medical Sciences
Full name of responsible person
Dr. Ayesheh Enayati
Position
Assistant Professor
Latest degree
Ph.D.
Other areas of specialty/work
Medical Pharmacy
Street address
Shastkola Road
City
Gorgan
Province
Golestan
Postal code
14395-477
Phone
+98 17 3245 1434
Email
enayati_phyto@yahoo.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Gorgan University of Medical Sciences
Full name of responsible person
Dr. Ayesheh Enayati
Position
Assistant Professor
Latest degree
Ph.D.
Other areas of specialty/work
Medical Pharmacy
Street address
Shastkola Road
City
Gorgan
Province
Golestan
Postal code
14395-477
Phone
+98 17 3245 1434
Email
enayati_phyto@yahoo.com

Person responsible for updating data

Contact
Name of organization / entity
Gorgan University of Medical Sciences
Full name of responsible person
Dr. Ayesheh Enayati
Position
Assistant Professor
Latest degree
Ph.D.
Other areas of specialty/work
Medical Pharmacy
Street address
Shastkola Road
City
Gorgan
Province
Golestan
Postal code
4918936316
Phone
+98 17 3245 1434
Email
enayati_phyto@yahoo.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
No - There is not a plan to make this available
Justification/reason for indecision/not sharing IPD
There is no more information.
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
No - There is not a plan to make this available
Informed Consent Form
No - There is not a plan to make this available
Clinical Study Report
No - There is not a plan to make this available
Analytic Code
No - There is not a plan to make this available
Data Dictionary
No - There is not a plan to make this available
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