Effects of Eight Weeks of High-Intensity Interval Training on Inflammatory Markers in Women with Non-Alcoholic Fatty Liver Disease: A Randomized Controlled Trial

This study aimed to evaluate the effects of eight weeks of High-Intensity Interval Training on inflammatory markers including C-Reactive Protein, Interleukin 6, and Cortisol, liver function, anthropometric measures including weight, body mass index, and waist-to-hip ratio, and insulin sensitivity in overweight and obese women with Non-Alcoholic Fatty Liver Disease

This randomized trial included 26 women with Non-Alcoholic Fatty Liver Disease confirmed by sonography, assigned using a closed-envelope method to a control group or an exercise intervention group performing (High-Intensity Interval Training).

The study will be conducted as an interventional trial at the gymnasium of Razi University in Kermanshah. All exercises will be supervised by the research team and an experienced trainer, performed in controlled environments with safety standards and accurate evaluation. The intervention will last eight weeks, and assessments will be conducted 48 hours before and after the intervention.

The study included 26 overweight and obese women aged 30 to 50 years, randomly assigned to exercise and control groups. Inclusion criteria were female sex, age 30–50 years, residence in Kermanshah, and body mass index between 25 and 35. Exclusion criteria were regular physical activity in the past three months, physical or musculoskeletal disabilities, use of liver-related or metabolic medications, and severe cardiovascular, pulmonary, renal, or musculoskeletal diseases.

The study included two groups: a control group and a High-Intensity Interval Training group.

The measured outcomes included C-Reactive Protein ,Interleukin-6, Cortisol, liver enzymes, lipid profile, and body composition

For the random allocation of participants to the study groups (Control group and High-Intensity Interval Training group), the sealed envelopes method was used. In this method, a random allocation sequence was first generated using random number software. Each allocation number was then assigned to a specific group (Control group or High-Intensity Interval Training group). These numbers were written on paper and placed into opaque, non-transparent envelopes. The envelopes were arranged completely randomly without any particular order. When each participant entered the study, one of the sealed envelopes was randomly selected and opened. Based on the number inside the envelope, the participant was assigned to one of the two groups. This method was implemented to ensure allocation concealment and to prevent bias from both the researchers and the participants.

Personal data of study participants can be shared after removing identifying information.

The access period will begin 6 months after the results are published.

The data will be available only to researchers affiliated with academic and scientific institutions.

The use of data is permitted only for conducting similar scientific research and must not be used for the personal interests of specific individuals or groups.

Zahra Sohrabi, Amir Abbas Monazami

The request should be sent to the provided email address; the applicant's request will be reviewed, and the data will be sent within 5–7 days if the conditions are deemed satisfactory.