Protocol summary

Study aim
Investigation of the safety and effectiveness of exosome derived from human umbilical cord jelly Wharton mesenchymal stem cells injection compared to intralesional triamcinolone injection in the treatment of alopecia areata: a randomized, blinded clinical trial
Design
This study is conducted as a phase 1 study on 20 patients with alopecia areata. In the intervention group, each patient will receive 3 sessions at 4-week intervals, with exosome injections in each session, and in the control group, each patient will receive triamcinolone injections in 3 sessions at 1-month intervals.
Settings and conduct
This project is being carried out at the Skin and Stem Cell Research Center of Tehran University of Medical Sciences. The intervention group will receive exosome injections, while the control group will receive triamcinolone injections.
Participants/Inclusion and exclusion criteria
Inclusion criteria: men and women with alopecia areata, age 18 years and older and less than 50 years, complete patient consent to participate in the project. Exclusion criteria: platelet disorders, thrombocytopenia, patients receiving anticoagulants, patients with malignancy, receiving chemotherapy in the last 5 years, patients with sepsis, smoking, pregnancy, ulcers or active infections at the treatment site.
Intervention groups
In the intervention group, patients receive three sessions, one month apart, of intradermal injection of one cc of exosomes at a concentration of 50 micrograms per milliliter in the area with hair loss, and in the control group, patients receive three sessions, one month apart, of intradermal injection of one cc of triamcinolone at a concentration of 2.5-5 mg per milliliter in the area with hair loss.
Main outcome variables
The amount of hair loss; side effects; patient's satisfaction; tolerability

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20200127046282N65
Registration date: 2025-12-26, 1404/10/05
Registration timing: prospective

Last update: 2025-12-26, 1404/10/05
Update count: 0
Registration date
2025-12-26, 1404/10/05
Registrant information
Name
Sona Zare
Name of organization / entity
Skin and Stem Cell Research Center, Tehran University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 21 2665 7438
Email address
sonazarebio@gmail.com
Recruitment status
recruiting
Funding source
Expected recruitment start date
2026-01-05, 1404/10/15
Expected recruitment end date
2026-06-20, 1405/03/30
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Investigation of the safety and effectiveness of exosome derived from human umbilical cord jelly Wharton mesenchymal stem cells injection compared to intralesional triamcinolone injection in the treatment of alopecia areata: a randomized, blinded clinical trial
Public title
Study of exosome injection in the treatment of alopecia areata
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Men and women with alopecia areata Age 18 years and older and less than 50 years Complete patient consent to participate in the project
Exclusion criteria:
Platelet disorders Thrombocytopenia Patients receiving anticoagulants Patients with malignancy Receiving chemotherapy during the last 5 years Patients with sepsis Being a smoker Pregnancy Active wound or infection at the treatment site Do not receive any topical or systemic medication in the last 3 months Women with hyperprolactinemia Women with hormonal disorders or polycystic ovaries
Age
From 18 years old to 49 years old
Gender
Both
Phase
1
Groups that have been masked
  • Outcome assessor
Sample size
Target sample size: 20
Randomization (investigator's opinion)
Not randomized
Randomization description
Blinding (investigator's opinion)
Single blinded
Blinding description
Outcome assessors will be considered blind.
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Working Group/Ethics Committee in Research Faculty of Medicine - Tehran University of Medical Scienc
Street address
Elkhebal St., Quds St., Porsina St., Faculty of Medicine
City
Tehran
Province
Tehran
Postal code
1461884513
Approval date
2025-12-21, 1404/09/30
Ethics committee reference number
IR.TUMS.MEDICINE.REC.1404.578

Health conditions studied

1

Description of health condition studied
alopecia areata
ICD-10 code
L63.9
ICD-10 code description
Alopecia areata, unspecified

Primary outcomes

1

Description
The amount of hair loss
Timepoint
Before treatment and three month after the last session
Method of measurement
Trichoscan

Secondary outcomes

1

Description
Side effects
Timepoint
One week after session
Method of measurement
Standard for pharmacovigilance research group in europe questionnaire and visit

2

Description
Satisfaction
Timepoint
Three months after the last session
Method of measurement
Patient consent questionnaire and visit

3

Description
Tolerability
Timepoint
Each session after transplantation
Method of measurement
Patient tolerance questionnaire and visit

Intervention groups

1

Description
Intervention group: Patients receive three sessions, one month apart, of intradermal injection of one cc of exosomes at a concentration of 50 micrograms/ml into the area with hair loss.
Category
Treatment - Other

2

Description
Control group: Patients receive three sessions, one month apart, of intradermal injection of one cc of triamcinolone at a concentration of 2.5-5 mg/ml into the area with hair loss.
Category
Treatment - Other

Recruitment centers

1

Recruitment center
Name of recruitment center
Clinic of Skin and Stem cell Research Center of Tehran University of Medical Sciences
Full name of responsible person
Mohammad Ali Nilforoushzadeh
Street address
No 4, Maryam Alley, South Kamraniyeh Street
City
Tehran
Province
Tehran
Postal code
1937957511
Phone
+98 21 2665 7438
Email
Dr_nilforoush@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Tehran University of Medical Sciences
Full name of responsible person
Ramin Kordi
Street address
Vice President for Research and Technology, 6th Floor, Central University Organization, corner of Qods Street, Keshavarz Boulevard
City
Tehran
Province
Tehran
Postal code
141765383761
Phone
+98 21 8163 3698
Email
vcr@tums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Tehran University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Skin and Stem cell Research Center of Tehran University of Medical Sciences
Full name of responsible person
Sona Zare
Position
Researcher
Latest degree
Ph.D.
Other areas of specialty/work
Cellular and Molecular biology
Street address
No 4, Maryam Alley, South Kamraniyeh Street
City
Tehran
Province
Tehran
Postal code
1937957511
Phone
+98 21 2665 7438
Email
sonazarebio@gmail.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Skin and Stem cell Research Center of Tehran University of Medical Sciences
Full name of responsible person
Mohammad Ali Nilforoushzadeh
Position
Professor
Latest degree
Subspecialist
Other areas of specialty/work
Dermatology
Street address
No 4, Maryam Alley, South Kamraniyeh Street
City
Tehran
Province
Tehran
Postal code
1937957511
Phone
+98 21 2665 7438
Email
Dr_nilforoush@yahoo.com

Person responsible for updating data

Contact
Name of organization / entity
Skin and Stem cell Research Center of Tehran University of Medical Sciences
Full name of responsible person
Sona Zare
Position
researcher
Latest degree
Ph.D.
Other areas of specialty/work
Cellular and Molecular Biology
Street address
No 4, Maryam Alley, South Kamraniyeh Street
City
Tehran
Province
Tehran
Postal code
1937957511
Phone
+98 21 2665 7438
Email
dr.sonazare@gmail.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Not applicable
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Not applicable
Data Dictionary
Not applicable
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