The effect of fatigue caused by functional training with different levels of movement complexity on cortico-muscular coherence in specific scales of effort perception in active women

The aim of the present study was to investigate the effect of fatigue induced by functional training with different levels of movement complexity (functional training based on simple or complex movements) compared to non-functional training during specific self-reported measures of perceived exertion (RPE=Rate of Perceived Exertion) on cortical-muscular coherence in active women.

A 3-group, double-blind, crossover clinical trial with simple, phaseless randomization on 15 subjects.

A scientific-research and fundamental study in the field of sports neurophysiology will be conducted in the sports physiology laboratory, with participants and laboratory experts blinded to this research.

The inclusion criteria for the present study included age range of 18 to 28 years, being active (150 to 300 minutes of moderate intensity or 75 to 150 minutes of vigorous intensity per week) (43), and normal body mass index (18 to 25 kg/m2). Other inclusion criteria for the present study included not having cardiovascular and neuromuscular diseases and musculoskeletal injuries. Also, not taking medications, supplements, tobacco, and alcohol were essential prerequisites for study entry. Regular menstrual cycles were required to control hormonal factors. Exclusion criteria for the present study included unwillingness to participate in any of the phases of the study, occurrence of any injury or discomfort during the exercise protocol, and inability to perform the exercises due to severe fatigue or other conditions that potentially affect exercise performance.

Protocol Group 1 Protocol Group 2 Protocol Group 3

Fatigue from functional training with different levels of movement complexity , effort perception scale, corticospinal coherence

Simple randomization with an individual and accessible unit will be performed from the statistical population, namely young active women living in Tehran, based on the SPSS statistical software. In this way, after collecting the samples using an accessible method and confirming the eligibility of each individual, a list of names or initial codes of all participants is entered into the SPSS environment. Then, a random number with a uniform distribution between zero and one is generated for each individual. Before generating these numbers, an initial Seed (for example, equivalent to the start date of the study) is set in the Random Number Generators section so that the randomization process is completely reproducible (repeatable). After sorting the generated numbers, by setting a cut point of 0.5, people whose random number is less than 0.50 are assigned to the intervention group and the rest (number greater than or equal to 0.50) are assigned to the control/proof group. This method is the simplest random assignment model in which each participant has a completely equal chance (50-50) of being placed in each group, and the assignment of individuals is completely independent of individual characteristics (such as age and physical activity history) and also independent of the researcher's judgment. Finally, to ensure the success of randomization, the balance of the distribution of background variables (such as mean age and body mass index) between the two groups is examined using t-tests or chi-square tests, and the results will be presented in the report

To blind the study according to the requirements of the ethics committee, participants were only informed of the general objectives of the study and remained unaware of the details of the interventions, grouping, and main hypotheses. In the laboratory section, all samples and raw data were labeled with multilayer random codes (including letters and numbers without a meaningful sequence) by an independent person (other than the operator and analyst) before being delivered to the experts, so that the experts were not aware of the allocation of samples to the intervention or control groups. The decryption key for these codes is kept in separate sealed envelopes with the data manager (who had no role in the initial collection and analysis process) and is not accessible until the database is finalized and the accuracy of the data entry is confirmed by the ethics committee, in order to ensure both observer bias and complete confidentiality of the data until the final locking (Data Lock).

EEG, neuromuscular, output data for statistical steps. Only 3 minutes of resting EEG data can be shared with participants.

Access period starts 1 year after results are published.

The data will be available to the supervisors-advisors and the student to complete the remaining stages of the thesis.

Only 3 minutes of resting EEG data can be made available to participants one year after printing.

Dr. Leila Ghazaleh with contact number +989125458969

If the legal authority for the research deems it appropriate, it will take about a week for the data to be made available to the participant after the data request process.