In this study, 60 healthy women aged from 20 to 50, along with their 1 to 6-month infants were selected from a Health Center in Birjand. The aim of this study was evaluating the effect of vitamin C and E supplements on the total antioxidant capacity of human’s milk. Exclusion criteria included the formula-feed for the infants and major illnesses (diabetes, infectious diseases, and genetic diseases) of both mothers and infants. Mothers and their infants were divided randomly and alternately into an experimental group and a control group. The mothers in the experimental and control groups were on free diet. Mothers in the experimental group were given 30 effervescent tablets of vitamin C (500 mg) and 30 chewable tablets of vitamin E (100 IU is equal to 100 mg). They should take one tablet from each two kinds of supplements on a daily basis. The control group did not receive this intervention. At the first step, before the intervention, milk samples of right breast of all the mothers in the two groups were taken manually from 9.00 to 11.00 a.m. when the infants were not breast-fed for at least one hour. Urine samples of the infants were collected by standard urine bags. The samples were taken 30 days later again. Total antioxidant capacity of milk and infants’ urine were measured by the FRAP method, and Free radical scavenging activity of the infants’ urine was measured by the DPPH method.