Study aim: We hypothesized that moderate aerobic training lowers the serum level of S100B and MDA or increases the serum level of SOD and improves headache indices and general health status in wMIG. Design: The study is a randomized controlled clinical trial (RCT) with a semi-experimental design (three groups with pre- and post-testing). Settings and conduct: Imam reza Hospital, Kermanshah Participants/Inclusion and exclusion criteria: The inclusion criteria were as follows: (1) no history of regular exercise in the previous 6 months; (2) no chronic diseases such as cardiovascular, hypertension, diabetes, arthritis, cancer, asthma, AIDS, meningitis or MS; (3) confirming headache by the neurologist; (4) Having an age range of 18 to 45 years. Exclusion criteria comprised: (1) situations that researchers understood during the research period, such as pregnancy, breastfeeding and abuse of drugs or alcohol; (2) participation in any additional exercise training programmes; (3) no interest on the part of the volunteer in continuing or making a change in their personal life schedule; (4) contracting COVID-19, and 5) voluntary finished the protocol. Intervention groups: 1) exercise+migraineurs group (doing 8 weeks of endurance exercise training, running and taking medicine prescribed by the doctor). 2) non-exercise+migraineurs group (only taking medicine prescribed by the doctor). 3. A healthy control group (without medicine and healthy). Main outcome variables: matrix metalloproteinases-2 (MMP-2), matrix metalloproteinases-9 (MMP-9), Inhibitors of matrix metalloproteinases-1(TIMP-1),Inhibitors of matrix metalloproteinases-2 (TIMP-2), S100 calcium-binding protein B(S100B)