Study aim: Determining the effect of home-based (HPT) and outdoor (OPT) Pilates training on 1. Improvement of Neurofilament Light Chain 2- Improvement of the Kynurenine pathway 3- Improvement of vitamin D 4- Improvement of physical function 5- Improvement of mental function In women with MS Design: A clinical trial with the control group, with parallel groups, double-blind, phase 2 on 44 patients. Excel software rand function was used for randomization. Settings and conduct: First, 44 people selected from all women with multiple sclerosis in Fasa and divided into three groups. The written consent was received from the subjects based on their voluntary and conscious participation in the training sessions. Before starting the training, two or three sessions of introduction, information about the benefits and possible side effects, as well as how to participate for patients and the required factors (height, weight, BMI, etc.) are measured. Anthropometric characteristics of . Also, cardiorespiratory endurance, muscular endurance, agility and muscle strength, and fatigue, quality of life and depression, as well as blood indicators including Nfl, Kynp And serum vitamin D were measured before and after eight weeks of fasting exercise. The place of blood sampling and analysis of the results were Fasa Medical Sciences Laboratory. Participants/Inclusion and exclusion criteria: Admission requirements include no smoking, no regular exerciseو at least two years of history Exclusion criteria include muscle injuries, severe recurrence of the disease, inability to perform the exercise Intervention groups: Intervention group 1: 8 weeks of home-based and outdoor Pilates training (3 sessions per week, 1hour each session) Control group: did not do any regular exercise for eight weeks and remained sedentary. Main outcome variables: Neurofilament light chain, Kynurenine pathway, serum Vitamin D, functional ability