History
# Registration date Revision Id
3 2020-06-19, 1399/03/30 139942
2 2020-05-18, 1399/02/29 137156
1 2017-04-10, 1396/01/21 2555
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  • Protocol summary

    The aim of the present study is to evaluate the effect of hydro-alcoholic extract of purslane in patients with non-alcoholic fatty liver disease (NAFLD). In a 12-week randomized, double-blind, clinical trial, 88 patients will be assigned to take either purslane extract capsule (containing 300 mg purslane extract) or placebo capsule. The study will be explained to each subject, who signs an informed consent. Steatosis grade will be determined by ultrasonography once at the beginning of the study and at the end of week 12. Three 24-hour recalls will be taken at the beginning and at the end of the study, including two working days and one weekend day. Diets will be analyzed for calorie and nutrient composition. Patient’s adherence to the study will be monitored every 15 days using telephone contact. Fasting blood samples will be obtained at baseline and at the end of study. Fasting blood sugar, insulin, total cholesterol, triglyceride, HDL-C, LDL-C, alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, alkaline phosphatase, glutathione peroxidase, total bilirubin, adiponectine, NF-κB and NF-κB gene expression will be assessed by appropriate methods. The study protocol has been approved by ethics committee of Iran University of Medical Sciences.
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    هدف مطالعه حاضر، ارزيابي اثر عصاره هيدروالکلي خرفه در بيماران مبتلا به کبد چرب غير الکلي (NAFLD) مي باشد. در يک کارآزمايي باليني تصادفي شده دو سوکور 12 هفته اي، 88 بيمار به دو گروه دريافت کننده کپسول عصاره خرفه (حاوي 300 ميلي گرم عصاره خرفه) يا کپسول دارونما تقسيم مي شوند. مطالعه براي تک تک بيماران شرح و فرم رضايتنامه کتبي از بيماران اخذ خواهد شد. درجه استئاتوز کبدي با استفاده از اولتراسونوگرافي، يکبار در ابتدا و بار ديگر در هفته 12 مطالعه تعيين مي شود. سه روز يادآمد غذايي در آغاز و پايان مطالعه، شامل دو روز کاري و يک روز تعطيل، گرفته خواهد شد. رژيم غذايي بيماران براي تعيين کالري و ترکيب مواد مغذي آناليز مي شود. تبعيت بيماران از مطالعه با تماس تلفني هر 15 روز يکبار مورد پايش قرار مي گيرد. نمونه هاي خون ناشتا در ابتدا و انتهاي مطالعه از بيماران گرفته خواهد شد. قند خون ناشتا، انسولين، کلسترول تام، تري گليسيريد، HDL-C، LDL-C، آلانين آمينوترانسفراز، آسپارتات آمينوترانسفراز، گاما گلوتاميل ترانسفراز، آلکالين فسفاتاز، گلوتاتيون پراکسيداز، بيلي روبين تام، آديپونکتين، NF-κB و بيان ژن NF-κB با استفاده از روشهاي مناسب ارزيابي مي شوند. پروتکل مطالعه توسط کميته اخلاق دانشگاه علوم پزشکي ايران تصويب شده است.
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    The aim of this study was to evaluate the effect of portulaca oleracea (purslane) hydroalcoholic extract in patients with non-alcoholic fatty liver disease (NAFLD).
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    هدف مطالعه حاضر، ارزيابي اثر عصاره هيدروالکلي خرفه در بيماران مبتلا به کبد چرب غير الکلي (NAFLD) مي باشد.
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    A 12-week randomized, double-blinded, parallel clinical trial on 74 patients with NAFLD.
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    کارآزمایی بالینی موازی تصادفی شده دو سوکور 12 هفته ای بر روی 74 بیمار مبتلا به کبد چرب غیر الکلی
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    Patients are randomly divided into intervention and placebo groups (37 patients in each group). The study process is described for each patient and a written consent form is obtained from the patients. The degree of hepatic steatosis is determined using ultrasound once at the beginning and again at week 12 of the study. The 3-day of 24-hour dietary recall is taken at the beginning and end of the study. A diet for 10% weight loss will be provided to each patients individually by a nutritionist. Patients' compliance is monitored by telephone every 15 days. Fasting blood samples are taken from patients at the beginning and at end of the study. The study protocol has been approved by the ethics committee of Iran University of Medical Sciences.
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    بیماران بطور تصادفی به دو گروه مداخله و دارونما تقسیم می شوند (37 بیمار در هر گروه). فرایند مطالعه براي تک تک بيماران شرح داده و فرم رضايتنامه کتبي از بيماران اخذ می گردد. درجه استئاتوز کبدي با استفاده از اولتراسونوگرافي، يکبار در ابتدا و بار ديگر در هفته 12 مطالعه تعيين مي شود. سه روز يادآمد غذايي در آغاز و پايان مطالعه گرفته مي شود. رژیم غذایی با هدف 10% کاهش وزن توسط متخصص تغذیه برای همه بیماران تنظیم می گردد. تبعيت بيماران از مطالعه با تماس تلفني هر 15 روز يکبار مورد پايش قرار مي گيرد. در ابتدا و انتهاي مطالعه، نمونه هاي خون ناشتا از بيماران گرفته می شود. پروتکل مطالعه توسط کميته اخلاق دانشگاه علوم پزشکي ايران تصويب شده است.
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    Men and women with non-alcoholic fatty liver disease whose ALT is greater than 30 U / l and more than 19 U / L, respectively, and who do not have chronic liver disease, cardiovascular disease, hypertension, or kidney stones are eligible to participate in the study.
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    مردان و زنان مبتلا به کبد چرب غیر الکلی که ALT آنها به ترتیب بيشتر از 30 U/l و بيشتر از U/L 19 بوده و فاقد بیماریهای کبدی مزمن، قلبی عروقی، پرفشاری خون و سنگهای کلیوی می باشند واجد شرکت در مطالعه هستند.
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    Intervention group: one capsule containing 300 mg of purslane hydroalcoholic extract daily. Placebo Group: A capsule daily similar in appearance to drug capsules, filled with toasted powder.
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    گروه مداخله: روزانه یک عدد کپسول حاوی 300 میلی گرم عصاره هیدروالکلی خرفه. گروه دارونما: روزانه یک کپسول با ظاهر مشابه کپسولهای دارو، پر شده با پودر سوخاری.
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    Serum levels of ALT and AST are the main outcomes of the study. Other variables include fasting blood glucose, insulin, total cholesterol, triglycerides, HDL-C, LDL-C, gamma glutamyl transferase, alkaline phosphatase, glutathione peroxidase, total bilirubin, adiponectin, NF-kB and expression of the NF-κB gene.
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    سطوح سرمی ALT و AST متغیرهای اصلی مطالعه هستند. سایر متغیرها عبارتند از: قند خون ناشتا، انسولين، کلسترول تام، تري گليسيريد، HDL-C، LDL-C، گاما گلوتاميل ترانسفراز، آلکالين فسفاتاز، گلوتاتيون پراکسيداز، بيلي روبين تام، آديپونکتين، NF-κB و بيان ژن NF-κB
  • General information

    1
    1
    88
    74
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    1. Submission of information in the old version of IRCT 2. Creating some changes in the sample size after the approval of the Vice Chancellor for Research of Iran University of Medical Sciences 3. Correcting the secondary outcome variables
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    1. ثبت اطلاعات در نسخه قدیمی IRCT 2. ایجاد بعضی تغییرات در حجم نمونه پس از موافقت معاونت پژوهشی دانشگاه علوم پزشکی ایران 3. تصحیح نگارش متغیرهای پیامد ثانویه
    The effect of hydro-alcoholic extract of Portulaca Oleracea L. (purslane) on Liver enzymes, Glycemic status, lipid pforile, Gluathione peroxidase activity, Adiponectin, NF-κB, and NF-κB gene expression in non-alcoholic fatty liver disease: a randomized, double-blind clinial trial.
    The effect of hydro-alcoholic extract of Portulaca Oleracea L. (purslane) on Liver enzymes, Glycemic status and lipid profile in non-alcoholic fatty liver disease: a randomized, double-blind clinical trial.
    اثر مصرف عصاره هيدروالکلي گياه خرفه بر آنزيمهاي کبدي، وضعیت گلایسمیک، پروفايل ليپيدي، فعالیت گلوتاتيون پراکسيداز، آديپونکتين، NF-κB و بيان ژن NF-κB در افراد مبتلا به کبد چرب غیر الکلی: کارآزمايي باليني تصادفي شده دوسوکور
    اثر مصرف عصاره هيدروالکلي گياه خرفه بر آنزيمهاي کبدي، وضعیت گلایسمیک و پروفايل ليپيدي در افراد مبتلا به کبد چرب غیر الکلی: کارآزمايي باليني تصادفي شده دوسوکور
    Being volunteer or Wishing to attend
    Age 18 years or older
    Alanine aminotransferase (ALT) greater than 19 IU/L for women and greater than 30 IU/L for men
    Evidence of fatty liver in ultrasonography with a score of 1 or more
    BMI ≥ 25 kg/m2
    تمایل به شرکت در مطالعه
    سن 18 سال به بالا
    سطوح سرمي آنزيم کبدي (ALT بيشتر از 19 U/l براي زنان و بيشتر از U/L 30 براي مردان)
    درجه استئاتوز کبدي يک و بالاتر در اولتراسونوگرافي بيماران
    BMI ≥ از 25 kg/m2
    Other acute or chronic hepatic disorders (hepatitis B or C)
    Biliary diseases
    Cancer
    Hypertension
    History of cardiovascular disease
    Nephrolithiasis (oxalate stones) or history of oxalate stones
    Consumption of omega-3 and vitamin E supplement
    Alcohol use
    Use of hepatotoxic medications during last month
    Changing medication dosage during the study
    Pregnancy
    Lactation
    Compliacne less than 80%
    ابتلا به ساير بيماريها و اختلالات مزمن و حاد کبدي (هپاتيت B، C)
    ابتلا به بيماريهاي صفراوي
    ابتلا به سرطان
    ابتلا به پرفشاری خون
    سابقه بیماری های قلبی- عروقی
    ابتلا به سنگ کليه از نوع اگزالاتي يا سابقه وجود سنگ کليه اگزالاتي
    مصرف مکملهاي امگا-3 و ويتامين E
    مصرف الکل
    مصرف داروهاي هپاتوتوکسيک طي يک ماه گذشته
    تغيير در دوز داروهاي مصرفي در طول مطالعه
    بارداری
    شیردهی
    کمپليانس کمتر از 80%
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    Block randomization method has been used for randomization. Blocks of size 4 are generated using www.sealedenvelope.com. In order to conceal in the randomization process, unique codes have been used on the medicine boxes, which are produced by the software.
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    برای تصادفی سازی از روش بلوکهای تصادفی استفاده شده است.بلوکهای چهارتایی با استفاده از سایت www.sealedenvelope.com تولید شده اند. به منظور اعمال پنهان سازی یا concealment در فرایند تصادفی سازی از کدهای منحصر به فرد بر روی جعبه های دارویی استفاده شده است که این کدها توسط نرم افزار تولید شده اند.
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    In order to apply concealment in the randomization process, unique codes will be used on the medicine boxes, and the desired code will also be produced by the software.
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    به منظور اعمال پنهان سازی در فرآیند تصادفی سازی از کدهای منحصر به فرد بر روی جعبه های دارویی استفاده خواهد شد که کد مورد نظر نیز توسط نرم افزار تولید می شود
    88 subjects
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    88 نفر
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  • Primary outcomes

    #1
    Laboratory kit (U/L)
    Laboratory kit
    کيت آزمايشگاهي (U/L)
    کيت آزمايشگاهي
    #2
    Laboratory kit (U/L)
    Laboratory kit
    کيت آزمايشگاهي (U/L)
    کيت آزمايشگاهي
  • Secondary outcomes

    #1
    Glutathione peroxidase (GPX)
    Weight
    گلوتاتيون پراکسيداز (GPX)
    وزن
    Laboratory kit (U/g Hb)
    Seca scale
    کيت آزمايشگاهي (U/g Hb)
    ترازوی Seca
    #2
    NF-κB
    Body mass index (BMI)
    NF-κB
    نمایه توده بدن
    Laboratory kit (ELISA method)
    Calculation: weight (in kilograms) divided by the square of height (in meters)
    کيت آزمايشگاهي (با روش ELISA)
    محاسبه: وزن (کیلوگرم) تقسیم بر قد (متر مربع)
    #3
    Adiponectin
    Waist circumference
    آديپونکتين
    دور کمر
    At the begining and at the end of week 12
    At the beginning and at the end of week 12
    Laboratory kit (ng/ml)
    Non-stretchable measuring tape
    کيت آزمايشگاهي (ng/ml)
    متر اندازه گیری غیر قابل ارتجاع
    #4
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    Liver steatosis
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    استئاتوز کبدی
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    At the beginning and at the end of week 12
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    در ابتداي مطالعه و در پايان هفته 12
    empty
    Sonography
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    سونوگرافی
    #5
    empty
    Systolic blood pressure (SBP)
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    فشار خون سیستولیک
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    At the beginning and at the end of week 12
    empty
    در ابتداي مطالعه و در پايان هفته 12
    empty
    Sphygmomanometer
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    فشارسنج
    #6
    empty
    Diastolic blood pressure (DBP)
    empty
    فشار خون دیاستولیک
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Sphygmomanometer
    empty
    فشارسنج
    #7
    empty
    Total cholesterol
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    کلسترول تام
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit
    empty
    کیت آزمایشگاهی
    #8
    empty
    Triglyceride (TG)
    empty
    تری گلیسیرید
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit
    empty
    کیت آزمایشگاهی
    #9
    empty
    High density lipoprotein (HDL)
    empty
    کلسترول با چگالی بالا (HDL)
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit
    empty
    کیت آزمایشگاهی
    #10
    empty
    Low density lipoprotein (LDL)
    empty
    کلسترول با دانسیته کم (LDL)
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit
    empty
    کیت آزمایشگاهی
    #11
    empty
    Albumin (ALB)
    empty
    آلبومین
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit
    empty
    کیت آزمایشگاهی
    #12
    empty
    Alkaline phosphatase (ALP)
    empty
    آلکالین فسفاتاز
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit
    empty
    کیت آزمایشگاهی
    #13
    empty
    Gamma glutamyl transferase (GGT)
    empty
    گاما گلوتامیل ترانسفراز
    empty
    At the begining and at the end of week 12
    empty
    در ابتداي مطالعه و در پايان هفته 12
    empty
    Laboratory kit
    empty
    کیت آزمایشگاهی
    #14
    empty
    Total bilirubin
    empty
    بیلیروبین تام
    empty
    At the beginning and at the end of week 12
    empty
    در ابتداي مطالعه و در پايان هفته 12
    empty
    Laboratory kit
    empty
    کيت آزمايشگاهي
    #15
    empty
    Fasting blood sugar (FBS)
    empty
    قند خون ناشتا
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit
    empty
    کیت آزمایشگاهی
    #16
    empty
    Insulin
    empty
    انسولین
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit (ELISA)
    empty
    کیت آزمایشگاهی (الایزا)
    #17
    empty
    Homeostatic model assessment of insulin resistance (HOMA-IR)
    empty
    مقاومت به انسولین
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Calculation: [fasting insulin (mU/L) * fasting blood glucose (mg/dl)]/405
    empty
    محاسبه: 405 ÷ ((mg/dl) قند خون ناشتا × (mU/L) انسولین ناشتا)
    #18
    empty
    Glutathione peroxidase activity
    empty
    فعالیت گلوتاتیون پراکسیداز
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit (ELISA)
    empty
    کیت آزمایشگاهی (الایزا)
    #19
    empty
    Adiponectin
    empty
    آدیپونکتین
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit (ELISA)
    empty
    کیت آزمایشگاهی (الایزا)
    #20
    empty
    Serum NF-kB concentration
    empty
    غلظت NF-kB سرم
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Laboratory kit (ELISA)
    empty
    کیت آزمایشگاهی (الایزا)
    #21
    empty
    NF-kB gene expression
    empty
    بیان ژن NF-kB
    empty
    At the beginning and at the end of week 12
    empty
    در ابتدای مطالعه و در پایان هفته 12
    empty
    Real Time RT-PCR
    empty
    Real Time RT-PCR
  • Recruitment centers

    #1
    Name of recruitment center - English: Rasoul akram Hospital
    Name of recruitment center - Persian: بيمارستان رسول اکرم
    Full name of responsible person - English: Dr. Shahram Agah
    Full name of responsible person - Persian: دکتر شهرام آگاه
    Street address - English: Niayesh St., Sattarkhan Ave., Rasoul akram Hospital.
    Street address - Persian: خيابان ستارخان، خيابان نيايش، بيمارستان رسول اکرم
    City - English: Tehran
    City - Persian: تهران
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:
    Name of recruitment center - English: Rasoul akram Hospital
    Name of recruitment center - Persian: بيمارستان رسول اکرم
    Full name of responsible person - English: Dr. Shahram Agah
    Full name of responsible person - Persian: دکتر شهرام آگاه
    Street address - English: Niayesh St., Sattarkhan Ave., Rasoul akram Hospital.
    Street address - Persian: خيابان ستارخان، خيابان نيايش، بيمارستان رسول اکرم
    City - English: Tehran
    City - Persian: تهران
    Province: Tehran
    Country: Iran (Islamic Republic of)
    Postal code: 1445613131
    Phone: +98 21 6435 1000
    Fax:
    Email: hrmc@iums.ac.ir
    Web page address: https://hrmc.iums.ac.ir/
  • Sponsors / Funding sources

    #1
    contact.organization_id:
    Name of organization / entity - English: Vice Chancellor for Research of Iran university Medical Sciences
    Name of organization / entity - Persian: معاونت پژوهشی دانشگاه علوم پزشکی ایران
    Full name of responsible person - English: Dr. Seyyed Ali Javad Mousavi, deputy head of Research and Technology, Iran University of Medical Sci
    Full name of responsible person - Persian: دکتر سید علی جواد موسوی ،معاون تحقیقات و فناوری، دانشگاه علوم پزشکی ایران
    Street address - English: Iran University of Medical Sciences, Shahid Hemmat Highway.
    Street address - Persian: بزرگراه همت، دانشگاه علوم پزشکی ایران
    City - English: Tehran
    City - Persian: تهران
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Dr. Seyyed Abbas Motevallian, deputy head of Research and Technology, Iran University of Medical Sci
    Full name of responsible person - Persian: دکتر سید عباس متولیان ،معاون تحقیقات و فناوری، دانشگاه علوم پزشکی ایران
    Street address - English: Iran University of Medical Sciences, Shahid Hemmat Highway.
    Street address - Persian: بزرگراه همت جنب برج میلاد، دانشگاه علوم پزشکی ایران
    City - English: Tehran
    City - Persian: تهران
    Province: Tehran
    Country: Iran (Islamic Republic of)
    Postal code: ۱۴۴۹۶۱۴۵۳۵
    Phone: +98 21 8670 2504
    Fax:
    Email: research-m@iums.ac.ir
    Web page address: https://vcr.iums.ac.ir
    empty
    Public
    empty
    Domestic
    empty
    Academic
    Vice Chancellor for Research of Iran university Medical Sciences
    Iran University of Medical Sciences
    معاونت پژوهشی دانشگاه علوم پزشکی ایران
    دانشگاه علوم پزشکی ایران
  • Person responsible for general inquiries

    contact.organization_id:
    Name of organization / entity - English: School of Health, Iran University of Medical Sciences
    Name of organization / entity - Persian: دانشکده بهداشت، دانشگاه علوم پزشکي ايران
    Full name of responsible person - English: Dr. Farzad Shidfar
    Full name of responsible person - Persian: دکتر فرزاد شيدفر
    Position - English: Full Professor
    Position - Persian: استاد
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Iran University of Medical Sciences, Shahid Hemmat Highway.
    Street address - Persian: بزرگراه همت، دانشگاه علوم پزشکی ایران
    City - English: Tehran
    City - Persian: تهران
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 1449614535
    Phone: 00982188607941- 00982188607945
    Mobile: +98 912 308 2922
    Fax: +98 21 8862 2707
    Email: Farzadshidfar@yahoo.com; Shidfar.f@iums.ac.ir
    Web page address:

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Dr. Farzad Shidfar
    Full name of responsible person - Persian: دکتر فرزاد شيدفر
    Position - English: Full Professor
    Position - Persian: استاد
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Iran University of Medical Sciences, Shahid Hemmat Highway.
    Street address - Persian: بزرگراه همت، دانشگاه علوم پزشکی ایران
    City - English: Tehran
    City - Persian: تهران
    Province: Tehran
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 1449614535
    Phone: 00982188607941- 00982188607945
    Mobile: +98 912 308 2922
    Fax: +98 21 8862 2707
    Email: Shidfar.f@iums.ac.ir
    Web page address:
  • Person responsible for scientific inquiries

    contact.organization_id:
    Name of organization / entity - English: Iran University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکي ايران
    Full name of responsible person - English: Dr. Farzad Shidfar
    Full name of responsible person - Persian: دکتر فرزاد شيدفر
    Position - English: PhD of Nutrition
    Position - Persian: دکتراي تخصصي تغذيه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: School of Health, Iran University of Medical Sciences, Shahid Hemmat Highway.
    Street address - Persian: بزرگراه همت، دانشگاه علوم پزشکی ایران، دانشکده بهداشت
    City - English: Tehran
    City - Persian: تهران
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 1449614535
    Phone: +98 21 8860 7945
    Mobile: +98 912 308 2922
    Fax: +98 21 8862 2707
    Email: Farzadshidfar@yahoo.com; Shidfar.f@iums.ac.ir
    Web page address:

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Dr. Farzad Shidfar
    Full name of responsible person - Persian: دکتر فرزاد شيدفر
    Position - English: PhD of Nutrition
    Position - Persian: دکتراي تخصصي تغذيه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: School of Health, Iran University of Medical Sciences, Shahid Hemmat Highway.
    Street address - Persian: بزرگراه همت، دانشگاه علوم پزشکی ایران، دانشکده بهداشت
    City - English: Tehran
    City - Persian: تهران
    Province: Tehran
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 1449614535
    Phone: +98 21 8860 7945
    Mobile: +98 912 308 2922
    Fax: +98 21 8862 2707
    Email: Farzadshidfar@yahoo.com
    Web page address:
  • Person responsible for updating data

    contact.organization_id:
    Name of organization / entity - English: Iran University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکي ايران
    Full name of responsible person - English: Dr. Farzad Shidfar
    Full name of responsible person - Persian: دکتر فرزاد شيدفر
    Position - English: PhD of Nutrition, Full Professor
    Position - Persian: دکتراي تخصصي تغذيه، استاد
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: School of Health, Iran University of Medical Sciences, Shahid Hemmat Highway.
    Street address - Persian: بزرگراه همت، دانشگاه علوم پزشکی ایران، دانشکده بهداشت
    City - English: Tehran
    City - Persian: تهران
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 1449614535
    Phone: +98 21 8860 7945
    Mobile: +98 912 308 2922
    Fax: +98 21 8862 2707
    Email: Farzadshidfar@yahoo.com; Shidfar.f@iums.ac.ir
    Web page address:

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Dr. Farzad Shidfar
    Full name of responsible person - Persian: دکتر فرزاد شيدفر
    Position - English: PhD of Nutrition, Full Professor
    Position - Persian: دکتراي تخصصي تغذيه، استاد
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: School of Health, Iran University of Medical Sciences, Shahid Hemmat Highway.
    Street address - Persian: بزرگراه همت، دانشگاه علوم پزشکی ایران، دانشکده بهداشت
    City - English: Tehran
    City - Persian: تهران
    Province: Tehran
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 1449614535
    Phone: +98 21 8860 7945
    Mobile: +98 912 308 2922
    Fax: +98 21 8862 2707
    Email: Farzadshidfar@yahoo.com
    Web page address:
  • Sharing plan

    no
    yes
    yes
    yes
    yes
    yes
    yes
    empty
    There is no further information
    empty
    اطلاعات بیشتری وجود ندارد
    empty
    Information on the main outcomes at the end of the study can be shared.
    empty
    اطلاعات مربوط به پیامدهای اصلی در انتهای مطالعه می تواند به اشتراک گذارده شود.
    empty
    The access period will be 6 months after the publication of the results.
    empty
    دوره دسترسی 6 ماه پس از چاپ نتایج خواهد بود.
    empty
    The data from this study will only be available to researchers working at academic and scientific institutions.
    empty
    داده های حاصل از این مطالعه فقط برای محققین شاغل در موسسات دانشگاهی و علمی در دسترس خواهد بود.
    empty
    Six months after the publication of the articles of this project, upon request from the corresponding author and his agreement, the study data can be made available to researchers.
    empty
    ۶ ماه پس از چاپ مقالات حاصل از داده های این طرح، با درخواست از مسئول طرح و موافقت ایشان، داده های مطالعه می تواند در دسترس محققین قرار گیرد.
    empty
    Applicants can contact the corresponding author via email or the following postal address to receive the required data. Nutrition department, School of health, Iran University of Medical Sciences, Hemmat highsway, Tehran Phon number:0098 21 8862 2755 E-mail: Farzadshidfar@yahoo.com
    empty
    متقاضیان برای دریافت داده های مورد نظر می توانند از طریق ایمیل یا آدرس پستی زیر با نویسنده مسئول مکاتبه کنند. آدرس پستی: تهران، بزرگراه همت، دانشگاه علوم پزشکی ایران، دانشکده بهداشت، گروه تغذیه شماره تلفن: 0098 21 8862 2755 ایمیل: Farzadshidfar@yahoo.com
    empty
    Applicants will be able to access the data from the present study no later than one week by sending an email to the corresponding author.
    empty
    متقاضیان از طریق ارسال ایمیل به نویسنده مسئول، حداکثر پس از گذشت یک هفته، قادر خواهند بود به داده های حاصل از مطالعه حاضر دسترسی داشته باشند.

Protocol summary

Study aim
The aim of this study was to evaluate the effect of portulaca oleracea (purslane) hydroalcoholic extract in patients with non-alcoholic fatty liver disease (NAFLD).
Design
A 12-week randomized, double-blinded, parallel clinical trial on 74 patients with NAFLD.
Settings and conduct
Patients are randomly divided into intervention and placebo groups (37 patients in each group). The study process is described for each patient and a written consent form is obtained from the patients. The degree of hepatic steatosis is determined using ultrasound once at the beginning and again at week 12 of the study. The 3-day of 24-hour dietary recall is taken at the beginning and end of the study. A diet for 10% weight loss will be provided to each patients individually by a nutritionist. Patients' compliance is monitored by telephone every 15 days. Fasting blood samples are taken from patients at the beginning and at end of the study. The study protocol has been approved by the ethics committee of Iran University of Medical Sciences.
Participants/Inclusion and exclusion criteria
Men and women with non-alcoholic fatty liver disease whose ALT is greater than 30 U / l and more than 19 U / L, respectively, and who do not have chronic liver disease, cardiovascular disease, hypertension, or kidney stones are eligible to participate in the study.
Intervention groups
Intervention group: one capsule containing 300 mg of purslane hydroalcoholic extract daily. Placebo Group: A capsule daily similar in appearance to drug capsules, filled with toasted powder.
Main outcome variables
Serum levels of ALT and AST are the main outcomes of the study. Other variables include fasting blood glucose, insulin, total cholesterol, triglycerides, HDL-C, LDL-C, gamma glutamyl transferase, alkaline phosphatase, glutathione peroxidase, total bilirubin, adiponectin, NF-kB and expression of the NF-κB gene.

General information

Reason for update
1. Submission of information in the old version of IRCT 2. Creating some changes in the sample size after the approval of the Vice Chancellor for Research of Iran University of Medical Sciences 3. Correcting the secondary outcome variables
Acronym
IRCT registration information
IRCT registration number: IRCT201701172709N44
Registration date: 2017-04-10, 1396/01/21
Registration timing: prospective

Last update: 2020-06-02, 1399/03/13
Update count: 2
Registration date
2017-04-10, 1396/01/21
Registrant information
Name
Farzad Shidfar
Name of organization / entity
Iran University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 21 8862 2755
Email address
shidfar.f@iums.ac.ir
Recruitment status
Recruitment complete
Funding source
Vice Chancellor for Research of Iran university of Medical Sciences
Expected recruitment start date
2017-06-22, 1396/04/01
Expected recruitment end date
2018-06-22, 1397/04/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of hydro-alcoholic extract of Portulaca Oleracea L. (purslane) on Liver enzymes, Glycemic status and lipid profile in non-alcoholic fatty liver disease: a randomized, double-blind clinical trial.
Public title
Effect of purslane extract on non-alcoholic fatty liver disease
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Being volunteer or Wishing to attend Age 18 years or older Alanine aminotransferase (ALT) greater than 19 IU/L for women and greater than 30 IU/L for men Evidence of fatty liver in ultrasonography with a score of 1 or more BMI ≥ 25 kg/m2
Exclusion criteria:
Other acute or chronic hepatic disorders (hepatitis B or C) Biliary diseases Cancer Hypertension History of cardiovascular disease Nephrolithiasis (oxalate stones) or history of oxalate stones Consumption of omega-3 and vitamin E supplement Alcohol use Use of hepatotoxic medications during last month Changing medication dosage during the study Pregnancy Lactation Compliacne less than 80%
Age
From 18 years old
Gender
Both
Phase
3
Groups that have been masked
  • Participant
  • Investigator
Sample size
Target sample size: 74
Randomization (investigator's opinion)
Randomized
Randomization description
Block randomization method has been used for randomization. Blocks of size 4 are generated using www.sealedenvelope.com. In order to conceal in the randomization process, unique codes have been used on the medicine boxes, which are produced by the software.
Blinding (investigator's opinion)
Double blinded
Blinding description
In order to apply concealment in the randomization process, unique codes will be used on the medicine boxes, and the desired code will also be produced by the software.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics Committee of Iran University of Medical Sciences
Street address
Iran University of Medical Sciences, Shahid Hemmat Highway.
City
Tehran
Province
Tehran
Postal code
1449614535
Approval date
2017-02-22, 1395/12/04
Ethics committee reference number
IR.IUMS.REC 1395.95-04-27-9221324202

Health conditions studied

1

Description of health condition studied
Nonalcoholic fatty liver disease
ICD-10 code
K76.0
ICD-10 code description
Fatty (change of) liver, not elsewhere classified

Primary outcomes

1

Description
Alanine aminotransferase (ALT)
Timepoint
At the begining and at the end of week 12
Method of measurement
Laboratory kit

2

Description
Aspartate aminotransferase (AST)
Timepoint
At the begining and at the end of week 12
Method of measurement
Laboratory kit

Secondary outcomes

1

Description
Weight
Timepoint
At the begining and at the end of week 12
Method of measurement
Seca scale

2

Description
Body mass index (BMI)
Timepoint
At the begining and at the end of week 12
Method of measurement
Calculation: weight (in kilograms) divided by the square of height (in meters)

3

Description
Waist circumference
Timepoint
At the beginning and at the end of week 12
Method of measurement
Non-stretchable measuring tape

4

Description
Liver steatosis
Timepoint
At the beginning and at the end of week 12
Method of measurement
Sonography

5

Description
Systolic blood pressure (SBP)
Timepoint
At the beginning and at the end of week 12
Method of measurement
Sphygmomanometer

6

Description
Diastolic blood pressure (DBP)
Timepoint
At the beginning and at the end of week 12
Method of measurement
Sphygmomanometer

7

Description
Total cholesterol
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit

8

Description
Triglyceride (TG)
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit

9

Description
High density lipoprotein (HDL)
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit

10

Description
Low density lipoprotein (LDL)
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit

11

Description
Albumin (ALB)
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit

12

Description
Alkaline phosphatase (ALP)
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit

13

Description
Gamma glutamyl transferase (GGT)
Timepoint
At the begining and at the end of week 12
Method of measurement
Laboratory kit

14

Description
Total bilirubin
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit

15

Description
Fasting blood sugar (FBS)
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit

16

Description
Insulin
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit (ELISA)

17

Description
Homeostatic model assessment of insulin resistance (HOMA-IR)
Timepoint
At the beginning and at the end of week 12
Method of measurement
Calculation: [fasting insulin (mU/L) * fasting blood glucose (mg/dl)]/405

18

Description
Glutathione peroxidase activity
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit (ELISA)

19

Description
Adiponectin
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit (ELISA)

20

Description
Serum NF-kB concentration
Timepoint
At the beginning and at the end of week 12
Method of measurement
Laboratory kit (ELISA)

21

Description
NF-kB gene expression
Timepoint
At the beginning and at the end of week 12
Method of measurement
Real Time RT-PCR

Intervention groups

1

Description
Intervention group: consumption of one capsule per day (containing 300 mg hydroethanolic extract of purslane) for 12 weeks
Category
Treatment - Other

2

Description
Control group: consumption of one placebo capsule for 12 weeks
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Rasoul akram Hospital
Full name of responsible person
Dr. Shahram Agah
Street address
Niayesh St., Sattarkhan Ave., Rasoul akram Hospital.
City
Tehran
Province
Tehran
Postal code
1445613131
Phone
+98 21 6435 1000
Email
hrmc@iums.ac.ir
Web page address
https://hrmc.iums.ac.ir/

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Iran University of Medical Sciences
Full name of responsible person
Dr. Seyyed Abbas Motevallian, deputy head of Research and Technology, Iran University of Medical Sci
Street address
Iran University of Medical Sciences, Shahid Hemmat Highway.
City
Tehran
Province
Tehran
Postal code
۱۴۴۹۶۱۴۵۳۵
Phone
+98 21 8670 2504
Fax
Email
research-m@iums.ac.ir
Web page address
https://vcr.iums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Iran University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Iran University of Medical Sciences
Full name of responsible person
Dr. Farzad Shidfar
Position
Full Professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Iran University of Medical Sciences, Shahid Hemmat Highway.
City
Tehran
Province
Tehran
Postal code
1449614535
Phone
00982188607941- 00982188607945
Fax
+98 21 8862 2707
Email
Shidfar.f@iums.ac.ir
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Iran University of Medical Sciences
Full name of responsible person
Dr. Farzad Shidfar
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
School of Health, Iran University of Medical Sciences, Shahid Hemmat Highway.
City
Tehran
Province
Tehran
Postal code
1449614535
Phone
+98 21 8860 7945
Fax
+98 21 8862 2707
Email
Farzadshidfar@yahoo.com
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Iran University of Medical Sciences
Full name of responsible person
Dr. Farzad Shidfar
Position
PhD of Nutrition, Full Professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
School of Health, Iran University of Medical Sciences, Shahid Hemmat Highway.
City
Tehran
Province
Tehran
Postal code
1449614535
Phone
+98 21 8860 7945
Fax
+98 21 8862 2707
Email
Farzadshidfar@yahoo.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
No - There is not a plan to make this available
Justification/reason for indecision/not sharing IPD
There is no further information
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Yes - There is a plan to make this available
Data Dictionary
Yes - There is a plan to make this available
Title and more details about the data/document
Information on the main outcomes at the end of the study can be shared.
When the data will become available and for how long
The access period will be 6 months after the publication of the results.
To whom data/document is available
The data from this study will only be available to researchers working at academic and scientific institutions.
Under which criteria data/document could be used
Six months after the publication of the articles of this project, upon request from the corresponding author and his agreement, the study data can be made available to researchers.
From where data/document is obtainable
Applicants can contact the corresponding author via email or the following postal address to receive the required data. Nutrition department, School of health, Iran University of Medical Sciences, Hemmat highsway, Tehran Phon number:0098 21 8862 2755 E-mail: Farzadshidfar@yahoo.com
What processes are involved for a request to access data/document
Applicants will be able to access the data from the present study no later than one week by sending an email to the corresponding author.
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