History
# Registration date Revision Id
6 2022-03-12, 1400/12/21 221687
5 2022-03-05, 1400/12/14 219353
4 2020-08-15, 1399/05/25 206152
3 2019-07-23, 1398/05/01 99241
2 2019-02-02, 1397/11/13 82505
1 2018-06-25, 1397/04/04 50488
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  • Protocol summary

    Effect of Nigella Sativa supplement on the expression of genes involved in insulin resistance using next generation methods (NGS), among obese women
    Effect of Nigella Sativa supplement on the expression of genes involved in insulin resistance among obese women
    بررسی اثر Nigella Sativa بر بیان ژن های مرتبط با مقاومت به انسولین در زنان چاق به روش توالی یابی نسل جدید (NGS)
    بررسی اثر Nigella Sativa بر بیان ژن های مرتبط با مقاومت به انسولین در زنان چاق
    Of the obese women referring to the Imam Ali Clinic in Yazd, 24 people will be selected and randomly divided into two groups. The participants and investigator will not be aware of the drugs and placebo. The amount of genes expression in the abdominal fat tissue will be measured at the end of each stage of the intervention by the next generation sequencing (NGS) method. Intervention is crossover and is performed in two stages of 8 weeks.
    Of the obese women referring to the Imam Ali Clinic in Yazd, 40 people will be selected and randomly divided into two groups. The participants and investigator will not be aware of the drugs and placebo. The amount of genes expression in PBMC will be measured before and after the end of each stage of the intervention. Intervention is crossover and is performed in two stages of 8 weeks.
    از بین زنان چاق مراجعه کننده به کلینیک امام علی شهر یزد، 24 نفر انتخاب خواهند شد و بصورت تصادفی به دو گروه تقسیم میشوند .محقق و شرکت کنندگان از دارو و دارونما اطلاع نخواهند داشت. میزان بیان ژنها در بافت چربی شکمی در پایان هر مرحله از مداخله به روش NGS اندازه گیری خواهد شد. مداخله متقاطع میباشد و به صورت دو مرحله 8 هفته ای انجام میگیرد.
    از بین زنان چاق مراجعه کننده به کلینیک امام علی شهر یزد، 40 نفر انتخاب خواهند شد و بصورت تصادفی به دو گروه تقسیم میشوند .محقق و شرکت کنندگان از دارو و دارونما اطلاع نخواهند داشت. میزان بیان ژنها در سلولهای تک هسته ای خون محیطی قبل و بعد از هر مرحله از مداخله اندازه گیری خواهد شد. مداخله متقاطع میباشد و به صورت دو مرحله 8 هفته ای انجام میگیرد.
    Inclusion criteria: Obese women with BMI between 30 to 35 kg/m2 and age between 25 to 55. Subjects should not use any drug. Should not have diabetes or pre-diabetes or dyslipidemia. Exclusion criteria: People who smoke or drink alcohol, pregnant and nursing women, People with chronic kidney or liver disease.
    Inclusion criteria: Obese women with BMI between 27 to 35 kg/m2 and age between 25 to 55. Subjects should not use any drug. Should not have diabetes or pre-diabetes or dyslipidemia. Exclusion criteria: People who smoke or drink alcohol, pregnant and nursing women, People with chronic kidney or liver disease.
    شرایط ورود: زنان چاق با شاخص توده بدنی 30 تا 35 کیلو گرم بر متر مربع و دامنه سنی 25 تا 55 سال. افراد نباید از هیچ دارویی استفاده نکنند و مبتلا به دیابت، پره دیابت و یا اختلالات چربی خون نباشند. شرایط عدم ورود: افرادي كه ازدخانيات و یا الكل استفاده مي كنند، زنان باردار و شیرده، افراد مبتلا به بيماري های مزمن كليوي، كبدي.
    شرایط ورود: زنان چاق با شاخص توده بدنی 27 تا 35 کیلو گرم بر متر مربع و دامنه سنی 25 تا 55 سال. افراد نباید از هیچ دارویی استفاده نکنند و مبتلا به دیابت، پره دیابت و یا اختلالات چربی خون نباشند. شرایط عدم ورود: افرادي كه ازدخانيات و یا الكل استفاده مي كنند، زنان باردار و شیرده، افراد مبتلا به بيماري های مزمن كليوي، كبدي.
    Expression of genes involved in insulin resistance in abdominal sub-cotaneouse adipose tissue; Expression of genes involved in insulin resistance in peripheral blood mononuclear cells; Fasting blood glucose; Serum insulin level; Anthropometric measurements (height, weight, BMI, waist circumference, hip circumference, waist to hip ratio); Body composition.
    Expression of genes involved in insulin resistance in peripheral blood mononuclear cells; Fasting blood glucose; Serum insulin level; Serum leptin level; Serum IL-6 level; Anthropometric measurements (height, weight, BMI, waist circumference, hip circumference, waist to hip ratio); Body composition.
    میزان بیان ژن های مرتبط با مقاومت به انسولین در بافت چربی زیر جلدی شکم؛ میزان بیان ژن های مرتبط با مقاومت به انسولین در سلول های تک هسته ای خون محیطی؛ قند خون ناشتا؛ سطح انسولین سرم؛ اندازه های آنتروپومتری (قد، وزن، شاخص توده بدنی، دور کمر، دور باسن، نسبت دور شکم به دور باسن)؛ ترکیب بدن؛
    میزان بیان ژن های مرتبط با مقاومت به انسولین در سلول های تک هسته ای خون محیطی؛ قند خون ناشتا؛ سطح انسولین سرم؛ سطح لپتین سرم؛ سطح IL-6 سرم؛ اندازه های آنتروپومتری (قد، وزن، شاخص توده بدنی، دور کمر، دور باسن، نسبت دور شکم به دور باسن)؛ ترکیب بدن؛
  • General information

    24
    40
    2
    1
    Effect of oral cumin (Nigella Sativa) supplement on the expression of genes involved in insulin resistance using Next Generation Sequencing (NGS) methods
    Effect of Nigella Sativa supplement on the expression of genes involved in insulin resistance
    بررسی اثر مکمل خوراکی زیره سیاه (Nigella Sativa) بر بیان ژن های مرتبط با مقاومت به انسولین به روش توالی یابی نسل جدید (NGS)
    بررسی اثر مکمل خوراکی Nigella Sativa بر بیان ژن های مرتبط با مقاومت به انسولین
    Effect of oral cumin (Nigella Sativa) supplement on the expression of genes involved in insulin resistance using Next Generation Sequencing (NGS) methods, and the measures of glycemic status and anthropometric indices among obese women
    Effect of Nigella Sativa supplement on the expression of genes involved in insulin resistance, glycemic status and anthropometric indices among obese women
    بررسی اثر مکمل خوراکی زیره سیاه (Nigella Sativa) بر بیان ژن های مرتبط با مقاومت به انسولین به روش توالی یابی نسل جدید (NGS)، مقادیر وضعیت گلایسمی و اندازه های آنتروپومتری در زنان چاق
    بررسی اثر مکمل خوراکی Nigella Sativa بر بیان ژنهای مرتبط با مقاومت به انسولین، وضعیت گلایسمی و اندازه های آنتروپومتری در زنان چاق
    Obese women with BMI between 30 to 35 kg/m2
    Age between 25 to 55
    Should not use any drug
    Should not have diabetes or pre-diabetes or dyslipidemia
    Obese women with BMI between 27 to 35 kg/m2
    Age between 25 to 55
    Should not use any drug
    Should not have diabetes or pre-diabetes or dyslipidemia
    زنان چاق با BMI بین 30 تا 35 kg/m2
    دامنه سنی 25 تا 55 سال می باشد
    نباید از هیچ دارویی استفاده نکنند
    مبتلا به دیابت، پره دیابت و یا اختلالات چربی خون نباشند
    زنان چاق با BMI بین 27 تا 35 kg/m2
    دامنه سنی 25 تا 55 سال می باشد
    نباید از هیچ دارویی استفاده نکنند
    مبتلا به دیابت، پره دیابت و یا اختلالات چربی خون نباشند
    Blood samples will be taken from all individuals before and after each period of interventions. From 5 people in each group, after the end of each period of the intervention, a subcutaneous adipose tissue sample will be taken from the abdominal region.
    Blood samples will be taken from all individuals before and after each period of interventions.
    از همه افراد قبل و بعد از هر مرحله از مداخله نمونه خون گرفته خواهد شداز 5 نفر در هر گروه، بعد از پایان هر دوره از مداخله نمونه بافت چربی زیرجلدی ناحیه شکم گرفته خواهد شد
    از همه افراد قبل و بعد از هر مرحله از مداخله نمونه خون گرفته خواهد شد
  • Primary outcomes

    #1
    Expression of genes involved in insulin resistance in abdominal sub-cotaneouse adipose tissue
    Expression of genes involved in insulin resistance in peripheral blood mononuclear cells
    میزان بیان ژن های مرتبط با مقاومت به انسولین در بافت چربی زیر جلدی شکم
    میزان بیان ژن های مرتبط با مقاومت به انسولین در سلول های تک هسته ای خون محیطی
    at the end of each 2-month intervention period
    at the beginning and end of each 2-month intervention period
    در انتهای هر دوره 2 ماهه مداخله
    در ابتدا و انتهای هر دوره 2 ماهه مداخله
    N.G.S. (next generation sequencing)
    Reverse transcription polymerase chain reaction (RT-PCR)
    توالی یابی نسل جدید
    واکنش زنجیره پلیمراز رونویسی معکوس
    #2
    Expression of genes involved in insulin resistance in peripheral blood mononuclear cells
    fasting blood glucose
    میزان بیان ژن های مرتبط با مقاومت به انسولین در سلول های تک هسته ای خون محیطی
    قند خون ناشتا
    در ابتدا و انتهای هر دوره 2 ماهه مداخله
    ابتدا و انتهای هر دوره 2 ماهه مداخله
    Reverse transcription polymerase chain reaction (RT-PCR)
    Biochemical analysis
    واکنش زنجیره پلیمراز رونویسی معکوس
    آنالیز بیوشیمیایی
    #3
    fasting blood glucose
    Serum insulin level
    قند خون ناشتا
    سطح انسولین سرم
    Biochemical analysis
    ELISA Kit
    آنالیز بیوشیمیایی
    کیت الایزا
    #4
    Serum insulin level
    Anthropometric measurements (height, weight, BMI, waist circumference, hip circumference, abdominal / hip ratio)
    سطح انسولین سرم
    اندازه های آنتروپومتری (قد، وزن، شاخص توده بدنی، دور کمر، دور باسن، نسبت دور شکم به دور باسن)
    ELISA Kit
    Meters and scales
    کیت الایزا
    متر و ترازو
    #5
    Anthropometric measurements (height, weight, BMI, waist circumference, hip circumference, abdominal / hip ratio)
    Body composition
    اندازه های آنتروپومتری (قد، وزن، شاخص توده بدنی، دور کمر، دور باسن، نسبت دور شکم به دور باسن)
    ترکیب بدن
    Meters and scales
    Body analyzer
    متر و ترازو
    دستگاه بادی آنالیزور
    #6
    Body composition
    serum leptin level
    ترکیب بدن
    سطح لپتین سرم
    Body analyzer
    ELISA Kit
    دستگاه بادی آنالیزور
    کیت الایزا
    #7
    empty
    serum IL-6 level
    empty
    سطح IL-6 سرم
    empty
    at the beginning and end of each 2-month intervention period
    empty
    ابتدا و انتهای هر دوره 2 ماهه مداخله
    empty
    ELISA Kit
    empty
    کیت الایزا
  • Sponsors / Funding sources

    #1

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: معاونت پژوهشی دانشکده بهداشت
    Full name of responsible person - Persian: Research Associate, School of Public Health
    Street address - English: Shohadaye gomnam Blvd., Campus of Shahid Sadoughi University of Medical Sciences , School of Public Health
    Street address - Persian: بلوار شهدای گمنام، پردیس دانشگاه علوم پزشکی شهید صدوقی یزد، دانشکده بهداشت
    City - English: Yazd
    City - Persian: یزد
    Province: Yazd
    Country: Iran (Islamic Republic of)
    Postal code: 8915173160
    Phone: +98 35 3820 9100
    Fax:
    Email: sphealth@ssu.ac.ir
    Web page address:

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Research Associate, School of Health
    Full name of responsible person - Persian: معاونت پژوهشی دانشکده بهداشت
    Street address - English: Shohadaye gomnam Blvd., Shahid Sadoughi University of Medical Sciences, School of Health
    Street address - Persian: بلوار شهدای گمنام، دانشگاه علوم پزشکی شهید صدوقی یزد، دانشکده بهداشت
    City - English: Yazd
    City - Persian: یزد
    Province: Yazd
    Country: Iran (Islamic Republic of)
    Postal code: 8915173160
    Phone: +98 35 3820 9100
    Fax:
    Email: sphealth@ssu.ac.ir
    Web page address:

Protocol summary

Study aim
Effect of Nigella Sativa supplement on the expression of genes involved in insulin resistance among obese women
Design
randomized double blind controlled clinical trial, cross-over
Settings and conduct
Of the obese women referring to the Imam Ali Clinic in Yazd, 40 people will be selected and randomly divided into two groups. The participants and investigator will not be aware of the drugs and placebo. The amount of genes expression in PBMC will be measured before and after the end of each stage of the intervention. Intervention is crossover and is performed in two stages of 8 weeks.
Participants/Inclusion and exclusion criteria
Inclusion criteria: Obese women with BMI between 27 to 35 kg/m2 and age between 25 to 55. Subjects should not use any drug. Should not have diabetes or pre-diabetes or dyslipidemia. Exclusion criteria: People who smoke or drink alcohol, pregnant and nursing women, People with chronic kidney or liver disease.
Intervention groups
Intervention group: capsule containing 1000 mg Nigella Sativa oil twice daily Control group: Placebo capsule containing paraffin oil twice daily
Main outcome variables
Expression of genes involved in insulin resistance in peripheral blood mononuclear cells; Fasting blood glucose; Serum insulin level; Serum leptin level; Serum IL-6 level; Anthropometric measurements (height, weight, BMI, waist circumference, hip circumference, waist to hip ratio); Body composition.

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20180430039475N1
Registration date: 2018-06-25, 1397/04/04
Registration timing: prospective

Last update: 2019-03-09, 1397/12/18
Update count: 5
Registration date
2018-06-25, 1397/04/04
Registrant information
Name
Sara Safi Esfahani
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 31 3230 5375
Email address
drsafi@ssu.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2018-07-23, 1397/05/01
Expected recruitment end date
2019-03-20, 1397/12/29
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Effect of Nigella Sativa supplement on the expression of genes involved in insulin resistance, glycemic status and anthropometric indices among obese women
Public title
Effect of Nigella Sativa supplement on the expression of genes involved in insulin resistance
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Obese women with BMI between 27 to 35 kg/m2 Age between 25 to 55 Should not use any drug Should not have diabetes or pre-diabetes or dyslipidemia
Exclusion criteria:
People who smoke or drink alcohol Pregnant and nursing women People with chronic kidney, liver disease
Age
From 25 years old to 55 years old
Gender
Female
Phase
3
Groups that have been masked
  • Participant
  • Investigator
  • Outcome assessor
Sample size
Target sample size: 40
More than 1 sample in each individual
Number of samples in each individual: 1
Blood samples will be taken from all individuals before and after each period of interventions.
Randomization (investigator's opinion)
Randomized
Randomization description
Randomization: Simple Random unit: Individual Tool: Randomized Tables A balanced block method is used to allocate concealment so that the number of samples assigned to each of the groups is equal.
Blinding (investigator's opinion)
Double blinded
Blinding description
Supplements of cumin and placebo in the same capsules in terms of size, color and shape Encodes A and B on supplements and placebo
Placebo
Used
Assignment
Crossover
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Shahid Sadoughi University of Medical Sciences Yazd
Street address
School of health, Sadoughi University of Medical Sciences, Shohadaye Gomnam Blvd., Alem Sq.
City
Yazd
Province
Yazd
Postal code
8915173160
Approval date
2018-04-17, 1397/01/28
Ethics committee reference number
IR.SSU.SPH.REC.1397.006

Health conditions studied

1

Description of health condition studied
Obesity
ICD-10 code
E66
ICD-10 code description
Overweight and obesity

Primary outcomes

1

Description
Expression of genes involved in insulin resistance in peripheral blood mononuclear cells
Timepoint
at the beginning and end of each 2-month intervention period
Method of measurement
Reverse transcription polymerase chain reaction (RT-PCR)

2

Description
fasting blood glucose
Timepoint
at the beginning and end of each 2-month intervention period
Method of measurement
Biochemical analysis

3

Description
Serum insulin level
Timepoint
at the beginning and end of each 2-month intervention period
Method of measurement
ELISA Kit

4

Description
Anthropometric measurements (height, weight, BMI, waist circumference, hip circumference, abdominal / hip ratio)
Timepoint
at the beginning and end of each 2-month intervention period
Method of measurement
Meters and scales

5

Description
Body composition
Timepoint
at the beginning and end of each 2-month intervention period
Method of measurement
Body analyzer

6

Description
serum leptin level
Timepoint
at the beginning and end of each 2-month intervention period
Method of measurement
ELISA Kit

7

Description
serum IL-6 level
Timepoint
at the beginning and end of each 2-month intervention period
Method of measurement
ELISA Kit

Secondary outcomes

empty

Intervention groups

1

Description
Intervention group: People in this group will recieve two 1000 mg capsules of Nigella Sativa daily for 8 weeks. Then it will be wash out period for 1 month, and after that, people will receive 2 placebo capsules daily for 8 weeks.
Category
Treatment - Drugs

2

Description
Control group:people in this group will recieve two placebo capsuls daily for 8 weeks. Then it will be wash out period for 1 month, and after that, people will receive two 1000 mg capsules of Nigella Sativa daily for 8 weeks.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Imam Ali clinic
Full name of responsible person
Sara Safi Esfahani
Street address
Navab Safavi Blvd.
City
Yazd
Province
Yazd
Postal code
8915173160
Phone
+98 35 3630 1700
Email
dr.s_safi@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Yazd University of Medical Sciences
Full name of responsible person
Research Associate, School of Health
Street address
Shohadaye gomnam Blvd., Shahid Sadoughi University of Medical Sciences, School of Health
City
Yazd
Province
Yazd
Postal code
8915173160
Phone
+98 35 3820 9100
Email
sphealth@ssu.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Yazd University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Yazd University of Medical Sciences
Full name of responsible person
Sara Safi Esfahani
Position
Phd Student
Latest degree
Medical doctor
Other areas of specialty/work
Nutrition
Street address
School of health, Shahid Sadoughi university of medical sciences, Shohadaye Gomnam Blvd., Alam Sq,
City
Yazd
Province
Yazd
Postal code
8915173160
Phone
+98 35 3820 9100
Email
drsafi@ssu.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Yazd University of Medical Sciences
Full name of responsible person
Azadeh Nadjarzadeh
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
School of health, Shahid Sadoughi university of medical sciences, Shohadaye Gomnam Blvd., Alam Sq,
City
Yazd
Province
Yazd
Postal code
8915173160
Phone
+98 35 3820 9100
Email
azadehnajarzadeh@gmail.com

Person responsible for updating data

Contact
Name of organization / entity
Yazd University of Medical Sciences
Full name of responsible person
Sara Safi Esfahani
Position
PhD Student
Latest degree
Medical doctor
Other areas of specialty/work
Nutrition
Street address
School of health, Shahid Sadoughi university of medical sciences, Shohadaye Gomnam Blvd., Alam Sq,
City
Yazd
Province
Yazd
Postal code
8915173160
Phone
+98 35 3820 9100
Email
drsafi@ssu.ac.ir

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Not applicable
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
Not applicable
Data Dictionary
Not applicable
Title and more details about the data/document
I have not yet decided on this.
When the data will become available and for how long
I have not yet decided on this.
To whom data/document is available
I have not yet decided on this.
Under which criteria data/document could be used
I have not yet decided on this.
From where data/document is obtainable
I have not yet decided on this.
What processes are involved for a request to access data/document
I have not yet decided on this.
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