History
# Registration date Revision Id
2 2019-09-24, 1398/07/02 105026
1 2019-02-11, 1397/11/22 78848
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  • Protocol summary

    Evaluation of the effect of curcuminoids supplementation on markers of insulin metabolism, biomarkers of inflammation and genes expression of TNF-α and IL-1 in patients with diabetic nephropathy
    The aim of this study is to determine the effects of Nano-curcumin supplementation on metabolic status in patients with diabetic hemodialysis.
    بررسی اثر مكمل ياري کورکومینوئید بر مارکر های متابولیسم انسولین،بیومارکر های التهابی و بیان ژن های TNF-α و IL-1 در بیماران مبتلا به نفروپاتي دیابتی
    هدف این مطالعه تعیین اثرات مکمل یاری نانو-کورکومین بر وضعیت متابولیک در بیماران مبتلا به همودیالیز دیابتیک است.
    In this research, 60 patients with diabetic nephropathy who are eligible will be selected. Participants are randomly divided into two groups of intervention and control by computer software. The design of the study is parallel blind randomized clinical trial
    Parallel double-blind (both patients and researchers) randomized controlled clinical trial. Random assignment will be done by the use of computer-generated random numbers.
    در این مطالعه 60 بیمار مبتلا به نفروپاتي ديابتي واجد شرایط مطالعه انتخاب می شوند. شرکت کنندگان بصورت تصادفی توسط نرم افزار کامپیوتر به دو گروه مداخله و کنترل تقسیم شده و طراحی مطالعه بصورت کارآزمایی بالینی تصادفی دوسویه کور موازی می باشد
    تصادفي کارآزمایی بالینی دو سوکور (هم بیماران و هم محققان) موازی. تصادفی سازی با استفاده از اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد.
    Sixty patients with diabetic hemodialysis who are eligible and are referred to dialysis center of Akhavan clinic affiliated to Kashan University of Medical Sciences, Kashan, Iran will be selected. Fasting blood samples will be taken at baseline and after 12 week intervention
    Akhavan Clinic, Kashan, Iran
    60 بیمار مبتلا به همودياليز ديابتيك واجد شرایط و ارجاع شده به بخش دياليز كلينيك اخوان وابسته به دانشگاه علوم پزشکی کاشان، کاشان ، ایران در مطالعه انتخاب خواهند شد. نمونه خون ناشتا در ابتدای مطالعه و 12 هفته بعد از مداخله از بیماران گرفته خواهد شد.
    كلينيك اخوان وابسته به دانشگاه علوم پزشکی کاشان، کاشان ، ایران
    Inclusion criteria: Diabetic hemodialysis patients; aged 40 to 85; ability to participate in the study until the end Exclusion criteria: Changing of the dosage of hypoglycemic drugs; use of other herbal medicines; unwillingness to cooperate
    Inclusion criteria: Diabetic hemodialysis patients; aged 18 to 80 Exclusion criteria: Patients suffering from infectious, inflammatory and malignant diseases. Patients taking curcumin supplements, antioxidant and/or anti-inflammatory supplements within 3 months before participation in the study. subjects receiving immunosuppressive and antibiotics medications.
    معيارهاي ورود: بیماران همودیالیزی دیابتیک، توانايي شركت در مطالعه تا پايان كار، سن 40 تا 80 سال معيارهاي خروج: تغییر در دوز داروهای پایین آورنده قند خون، استفاده از ساير داروهاي گياهي، عدم تمايل به همكاري
    معيارهاي ورود: بیماران همودیالیزی دیابتیک، سن 18 تا 80 سال معيارهاي خروج: بیماران مبتلا به عفونت، بیماریهای التهابی و بدخیمی ها بیمارانی که در طول سه ماه قبل از شروع این مطالعه مکمل کورکومین، مکملهای آنتی اکسیدانی و یا ضد التهای مصرف کرده اند. بیمارانی که داروهای سرکوب کننده ایمنی و آنتی بادی مصرف کرده اند
    Patients will be assigned to receive either curcuminoids (intervention group: n=30) or placebo (control group: n=30).
    Patients will be assigned to receive Nano-curcumin supplements (n=30) and placebo (n=30).
    بیماران برای دریافت كوركومينوئيد (گروه مداخله: n=30) یا پلاسبو (گروه کنترل: n=30) تقسیم خواهند شد.
    بیماران برای دریافت مکمل یاری نانو-کورکومین (30=n) و پلاسبو ( 30=n) تقسیم خواهند شد.
    Markers of insulin metabolism (primary outcomes) and inflammatory factors, oxidative stress biomarkers and gene expression related to inflammation (secondary outcomes) will be quantified at study baseline and end-of-trial.
    Fasting blood samples will be taken at baseline and after 12-week intervention. Insulin and insulin resistance as primary outcomes and inflammatory factors, biomarkers of oxidative stress, and serum lipoproteins as secondary outcomes will be measured at baseline and end-of-trial.
    مارکرهای متابولیسم انسولین (پیامدهای اولیه) و فاکتورهای التهابی و بیومارکرهای استرس اکسیداتیو و بیان ژن مرتبط با التهاب (پیامدهای ثانویه) در ابتدا و انتهای مداخله اندازه گیری خواهد شد.
    نمونه خون ناشتا در ابتدای مطالعه و 12 هفته بعد از مداخله از بیماران گرفته خواهد شد. انسولین و مقاومت به انسولین به عنوان پیامدهای اولیه و فاکتورهای التهابی، بیومارکرهای استرس اکسیداتیو و لیپوپروتئینهای سرم به عنوان پیامدهای ثانویه در ابتدا و انتهای مداخله اندازه گیری خواهد شد.
  • General information

    40
    18
    85
    80
    1
    1
    2018-11-16, 1397/08/25
    2018-11-26, 1397/09/05
    The effect of curcuminoids in patients with diabetic hemodialysis
    The effect of Nano-curcumin in patients with diabetic hemodialysis
    تاثير كوركومينوئيد در مبتلايان به همودياليز ديابتيك
    تاثير نانو-کورکومین در مبتلايان به همودياليز ديابتيك
    Evaluation of the effect of curcuminoids supplementation on markers of insulin metabolism, biomarkers of inflammation and genes expression of TNF- and IL-1 in patients with diabetic hemodialysis
    The effects of Nano-curcumin on metabolic status in patients with diabetes on hemodialysis
    بررسی اثر مكمل ياري کورکومینوئید بر مارکر های متابولیسم انسولین، بیومارکر های التهابی و بیان ژن های TNF-α و IL-1 در بیماران مبتلا به همودياليز ديابتيك
    بررسی اثر مكمل ياري نانو-کورکومین بر وضعیت متابولیک در بیماران مبتلا به همودياليز ديابتيك
    Diabetic hemodialysis patients
    Ability to participate in the study until the end
    Aged 40 to 80 years
    Diabetic hemodialysis patients
    Aged 18to 80 years
    بیماران همودیالیزی دیابتیک
    توانايي شركت در مطالعه تا پايان كار
    سن 40 تا 80 سال
    بیماران همودیالیزی دیابتیک
    سن 18 تا 80 سال
    Changing of the dosage of hypoglycemic drugs
    Use of other herbal medicines
    Unwillingness to cooperate
    Patients suffering from infectious, inflammatory and malignant diseases.
    Patients taking curcumin supplements, antioxidant and/or anti-inflammatory supplements within 3 months before participation in the study.
    subjects receiving immunosuppressive and antibiotics medications.
    تغییر در دوز داروهای پایین آورنده قند خون
    استفاده از ساير داروهاي گياهي
    عدم تمايل به همكاري
    بیماران مبتلا به عفونت، بیماریهای التهابی و بدخیمی ها
    بیمارانی که در طول سه ماه قبل از شروع این مطالعه مکمل کورکومین، مکملهای آنتی اکسیدانی و یا ضد التهای مصرف کرده اند.
    بیمارانی که داروهای سرکوب کننده ایمنی و آنتی بادی مصرف کرده اند.
    Randomization will be performed with simple method and random numbers generated by computer software which choose the random numbers. then we consider the specific numbers for both groups for example: the even numbers are for intervention group and the odd numbers are for the placebo group.
    At study baseline, after balanced blocked randomisation, particpants will be allocated into two groups to take either Nano-curcumin supplements (n = 30) or placebos (n = 30). Randomization will be done by the use of computer-generated random numbers.
    نحوه تصادفی سازی با روش ساده و استفاده از اعداد تصادفی ایجاد شده توسط نرم افزار کامپیوتری انجام خواهد شد. در اين روش كامپيوتر اعداد تصادفي انتخاب ميكند. با در نظرگرفتن اعداد براي دو گروه تصادف سازي انجام ميگيرد. مثلا اعداد زوج در گروه مداخله و اعداد فرد در گروه شاهد قرار خواهند گرفت.
    در ابتدای مطالعه، بعد از تصادفی سازی بلوکی بالانس شده، افراد به دو گروه برای دریافت مکمل نانو-کورکومین (n = 30) یا پلاسبو (n = 30) تخصیص داده خواهند شد. تصادفی سازی با استفاده از جدول اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد.
    In this study, participants and researchers are not aware of which group is placebo or supplemented curcuminoids groups.
    Randomization and allocation will be concealed from the researchers and participants until the final analyses are completed. Another person who is not involved in the trial and not aware of random sequences, will be assigned the participants to the numbered bottles of capsules.
    در این مطالعه شرکت کنندگان و محققان، از اینکه کدام گروه پلاسبو و کدام گروه مکمل كوركومينوئيد می گیرند اطلاعی ندارند.
    تصادفی سازی و تخصیص تصادفی از محققان و شرکت کنندگان در مطالعه تا زمان تکمیل آنالیز آماری پنهان خواهد ماند. فرد دیگری که در این کارآزمایی بالینی نقشی ندارد و از تخصیص تصادفی آگاه نیست، به شرکت کنندگان در مطالعه، بطری های شماره گذاری شده کپسول ها را تخصیص خواهد داد.
  • Secondary outcomes

    #1
    C-reactive protein (CRP)
    High sensitive-C-reactive protein (hs-CRP)
    #2
    Nitric Oxide
    Total Nitric Oxide
    #3
    Expressed levels of Tumor Necrosis Factor-α (TNF-α) gene
    Expression levels of PPAR-γ gene
    سطوح بیان (Tumor Necrosis Factor-α (TNF-α
    سطوح بیان PPAR-γ
    #4
    Expressed levels of InterLeukin-1 (IL-1) gene
    Expression levels of LDLR gene
    سطوح بیان (InterLeukin-1 (IL-1
    سطوح بیان ژن LDLR
    #5
    empty
    Expression levels of TGF-β gene
    empty
    سطوح بیان ژن TGF-β
    empty
    At the beginning of the study and 12 weeks after intervention
    empty
    در ابتدای مطالعه و 12 هفته بعد از مداخله
    empty
    Real-Time PCR Method
    empty
    Real-Time PCR
    #6
    empty
    Fasting blood sugar
    empty
    قند خون ناشتا
    empty
    At the beginning of the study and after 12 weeks of intervention
    empty
    در ابتدای مطالعه و 12 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #7
    empty
    VLDL
    empty
    VLDL
    empty
    At the beginning of the study and after 12 weeks of intervention
    empty
    در ابتدای مطالعه و 12 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #8
    empty
    LDL
    empty
    LDL
    empty
    At the beginning of the study and after 12 weeks of intervention
    empty
    در ابتدای مطالعه و 12 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #9
    empty
    BUN
    empty
    نیتروژن اوره خون
    empty
    At the beginning of the study and after 12 weeks of intervention
    empty
    در ابتدای مطالعه و 12 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #10
    empty
    Creatinine
    empty
    کراتینین
    empty
    At the beginning of the study and after 12 weeks of intervention
    empty
    در ابتدای مطالعه و 12 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #11
    empty
    AGEs
    empty
    AGEs
    empty
    At the beginning of the study and after 12 weeks of intervention
    empty
    در ابتدای مطالعه و 12 هفته بعد از مداخله
    empty
    Spectrophotometry
    empty
    اسپکتروفتومتری
    #12
    empty
    Systolic blood pressure (SBP)
    empty
    فشار خون سیستولیک
    empty
    At the beginning of the study and after 12 weeks of intervention
    empty
    در ابتدای مطالعه و 12 هفته بعد از مداخله
    empty
    Sphygmomanometer
    empty
    اسفیگمومانومتر
    #13
    empty
    Diastolic blood pressure (DBP)
    empty
    فشار خون دیاستولیک
    empty
    At the beginning of the study and after 12 weeks of intervention
    empty
    در ابتدای مطالعه و 12 هفته بعد از مداخله
    empty
    Sphygmomanometer
    empty
    اسفیگمومانومتر
  • Intervention groups

    #1
    Intervention group: receiving one capsule per day for 12 weeks (each capsule contains 80 mg curcuminoids)
    Intervention group: receiving one 80 mg Nano-curcumin capsule per day for 12 weeks
    گروه مداخله: دریافت روزانه يك کپسول 80 ميلي گرمی كوركومينوئيد به مدت 12 هفته
    گروه مداخله: کپسول 80 ميلي گرمی نانو-کورکومین روزانه یک عدد به مدت 12 هفته
    #2
    Control group: receiving one capsule of placebo per day for 12 weeks
    Control group: Placebo oral capsule, daily, for 12 weeks.
    گروه کنترل: دریافت روزانه يك کپسول پلاسبو به مدت 12 هفته
    پلاسبو، روزانه، به صورت خوراکی برای 12 هفته.

Protocol summary

Study aim
The aim of this study is to determine the effects of Nano-curcumin supplementation on metabolic status in patients with diabetic hemodialysis.
Design
Parallel double-blind (both patients and researchers) randomized controlled clinical trial. Random assignment will be done by the use of computer-generated random numbers.
Settings and conduct
Akhavan Clinic, Kashan, Iran
Participants/Inclusion and exclusion criteria
Inclusion criteria: Diabetic hemodialysis patients; aged 18 to 80 Exclusion criteria: Patients suffering from infectious, inflammatory and malignant diseases. Patients taking curcumin supplements, antioxidant and/or anti-inflammatory supplements within 3 months before participation in the study. subjects receiving immunosuppressive and antibiotics medications.
Intervention groups
Patients will be assigned to receive Nano-curcumin supplements (n=30) and placebo (n=30).
Main outcome variables
Fasting blood samples will be taken at baseline and after 12-week intervention. Insulin and insulin resistance as primary outcomes and inflammatory factors, biomarkers of oxidative stress, and serum lipoproteins as secondary outcomes will be measured at baseline and end-of-trial.

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20150606022562N6
Registration date: 2019-02-11, 1397/11/22
Registration timing: retrospective

Last update: 2019-09-25, 1398/07/03
Update count: 1
Registration date
2019-02-11, 1397/11/22
Registrant information
Name
Fereshteh Bahmani
Name of organization / entity
Kashan University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 31 5554 0021
Email address
fbahmani@kaums.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2018-11-06, 1397/08/15
Expected recruitment end date
2018-11-26, 1397/09/05
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effects of Nano-curcumin on metabolic status in patients with diabetes on hemodialysis
Public title
The effect of Nano-curcumin in patients with diabetic hemodialysis
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Diabetic hemodialysis patients Aged 18to 80 years
Exclusion criteria:
Patients suffering from infectious, inflammatory and malignant diseases. Patients taking curcumin supplements, antioxidant and/or anti-inflammatory supplements within 3 months before participation in the study. subjects receiving immunosuppressive and antibiotics medications.
Age
From 18 years old to 80 years old
Gender
Both
Phase
3
Groups that have been masked
  • Participant
  • Investigator
  • Outcome assessor
Sample size
Target sample size: 60
Randomization (investigator's opinion)
Randomized
Randomization description
At study baseline, after balanced blocked randomisation, particpants will be allocated into two groups to take either Nano-curcumin supplements (n = 30) or placebos (n = 30). Randomization will be done by the use of computer-generated random numbers.
Blinding (investigator's opinion)
Double blinded
Blinding description
Randomization and allocation will be concealed from the researchers and participants until the final analyses are completed. Another person who is not involved in the trial and not aware of random sequences, will be assigned the participants to the numbered bottles of capsules.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics Committee of Kashan University of Medical Sciences and Health Services
Street address
Kashan University of Medical Sciences, Ravand road
City
Kashan
Province
Isfehan
Postal code
8715988141
Approval date
2018-11-05, 1397/08/14
Ethics committee reference number
IR.KAUMS.MEDNT.REC.1397.065

Health conditions studied

1

Description of health condition studied
Chronic kidney disease
ICD-10 code
N18.5
ICD-10 code description
Chronic kidney disease, stage 5

Primary outcomes

1

Description
Insulin
Timepoint
At the beginning of the study and 12 weeks after intervention
Method of measurement
Elisa kit

2

Description
Insulin resistance
Timepoint
At the beginning of the study and after 24 weeks of intervention
Method of measurement
Calculation using HOMA formula

Secondary outcomes

1

Description
Triglycerides
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Enzymatic kit

2

Description
Total cholesterol
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Enzymatic kit

3

Description
High-density lipoprotein-cholesterol (HDL-Cholesterol)
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Enzymatic kit

4

Description
High sensitive-C-reactive protein (hs-CRP)
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Elisa kit

5

Description
Total Nitric Oxide
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Spectrophotometry

6

Description
Expression levels of PPAR-γ gene
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Real-Time PCR

7

Description
Expression levels of LDLR gene
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Real-Time PCR Method

8

Description
Gluthatione
Timepoint
At the beginning of the study and 12 weeks after intervention
Method of measurement
Spectrophotometry

9

Description
Total antioxidant capacity
Timepoint
At the beginning of the study and 12 weeks after intervention
Method of measurement
Spectrophotometry

10

Description
Malondialdehyde
Timepoint
At the beginning of the study and 12 weeks after intervention
Method of measurement
Spectrophotometry

11

Description
Expression levels of TGF-β gene
Timepoint
At the beginning of the study and 12 weeks after intervention
Method of measurement
Real-Time PCR Method

12

Description
Fasting blood sugar
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Enzymatic kit

13

Description
VLDL
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Enzymatic kit

14

Description
LDL
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Enzymatic kit

15

Description
BUN
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Enzymatic kit

16

Description
Creatinine
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Enzymatic kit

17

Description
AGEs
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Spectrophotometry

18

Description
Systolic blood pressure (SBP)
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Sphygmomanometer

19

Description
Diastolic blood pressure (DBP)
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Sphygmomanometer

Intervention groups

1

Description
Intervention group: receiving one 80 mg Nano-curcumin capsule per day for 12 weeks
Category
Treatment - Drugs

2

Description
Control group: Placebo oral capsule, daily, for 12 weeks.
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Akhavan clinin
Full name of responsible person
Fereshteh Bahmani
Street address
Ghotb-ravandi boulevard
City
Kashan
Province
Isfehan
Postal code
8719674591
Phone
+98 31 5534 2020
Fax
+98 31 5534 2025
Email
fbahmani@kaums.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Dr. Hamid Reza Banafshe
Street address
Vice chancellor for research, Kashan University of Medical Sciences ,Ravand road
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5554 2999
Email
banafsheh_h@kaums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Kashan University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Fereshteh Bahmani
Position
Associate Professor/ Academic staff member of the Department of Clinical Biochemistry
Latest degree
Ph.D.
Other areas of specialty/work
Biochemistry
Street address
Medical Faculty, Kashan University of Medical Sciences, Ravand road
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5554 0021
Email
bahmani@kaums.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Fereshteh Bahmani
Position
Associate Professor/ Academic staff member of the Department of Clinical Biochemistry
Latest degree
Ph.D.
Other areas of specialty/work
Biochemistry
Street address
Medical Faculty, Kashan University of Medical Sciences, Ravand road
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5554 0021
Email
fbahmani@kaums.ac.ir

Person responsible for updating data

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Fereshteh Bahmani
Position
Associate Professor/ Academic staff member of the Department of Clinical Biochemistry
Latest degree
Ph.D.
Other areas of specialty/work
Biochemistry
Street address
Medical Faculty, Kashan University of Medical Sciences, Ravand road
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5554 0021
Email
fbahmani@kaums.ac.ir

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
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