Evaluation of the effect of 12g l-citrulline supplementation before acute eccentric exercise on the response of antioxidant markers in humans
Design
A double-blind randomized, placebo-controlled, and crossover design
Settings and conduct
After receiving participant's written and signed consent forms, their anthropometric factors such as weight, height, and body fat percentage are measured. Due to having supplementation, the researcher and participants are blinded to minimize biases. Blinding is done by the researcher assistant with coding while he knows no information about the purpose of the study and the process. Subjects consume 12g l-citrulline or placebo 1-2 hours before the start of eccentric exercise. Before exercise, first blood samples are taken from the brachial vein, after that subjects perform the protocol (16kg KettleBell swing exercise) for 12 minutes with 12 sets; then other blood samples are taken after exercise, post 10, and post 30 minutes after exercise.
Participants/Inclusion and exclusion criteria
The healthy and trained male students of the University of Guilan between 20 and 23 years old enter the study. Participants must be free of disease and injury, no history of surgery and musculoskeletal disorder during the last two years, and no history of ergogenic aids, supplements, and drug use in the last 6 months leading to the study.
Intervention groups
Intervention group: receiving l-citrulline supplementation before acute eccentric exercise ( subjects consume a solution of 12g of l-citrulline powder with water 1 to 2 hours before the start of the exercise session). Control group: receiving maltodextrin before acute eccentric exercise ( subjects consume a solution of 12g of maltodextrin powder with water 1 to 2 hours before the start of the exercise session).
The effect of l-citrulline supplementation on nitric oxide and antioxidant markers after eccentric exercise in humans
Public title
Effect of L-Citrulline after eccentric exercise
Purpose
Basic scienece
Inclusion/Exclusion criteria
Inclusion criteria:
All healthy, trained male students at the University of Guilan
Exclusion criteria:
Being disease and injury free
No history of surgery and musculoskeletal disorder during the last two years
No history of ergogenic aids, supplements and drug use for in the last 6 months leading to the study
Age
From 20 years old to 23 years old
Gender
Male
Phase
N/A
Groups that have been masked
Participant
Investigator
Sample size
Target sample size:
12
Actual sample size reached:
9
Randomization (investigator's opinion)
Randomized
Randomization description
By using the lottery method, we prepare 9 sheets numbered 1to 9 that the even numbers belong to the control group and the odd numbers belong to the supplement group. Then, the lottery is done by participants. Randomization is determined by the researcher's assistant in the form of a double-blind design. Due to the crossover study, the study participants will be switched throughout to the second groups (supplement or placebo) after a washout period.
Blinding (investigator's opinion)
Double blinded
Blinding description
Both the researcher and participants did not know if participants were receiving the supplement or a placebo in the training sessions.
Placebo
Used
Assignment
Crossover
Other design features
Secondary Ids
empty
Ethics committees
1
Ethics committee
Name of ethics committee
Ethics committee of Guilan University of Medical Science
Before exercise, immediately after , post 10, and post 30 after exercise
Method of measurement
Blood sampling
Secondary outcomes
empty
Intervention groups
1
Description
Intervention group: participants consume a solution of 12g of l-citrulline powder supplementation with water 1 to 2 hours before the start of the exercise. Control group: participants consume a solution of 12g of maltodextrin powder with water 1 to 2 hours before the start of the exercise.
Category
Placebo
Recruitment centers
1
Recruitment center
Name of recruitment center
Guilan University
Full name of responsible person
Kosar Valaei
Street address
No. 14, Farhang Ave., Talebamoli Blvd., Amol Twon
City
Amol
Province
Mazandaran
Postal code
4615973556
Phone
+98 11 4428 9094
Email
kosar.valaei94@gmail.com
Sponsors / Funding sources
1
Sponsor
Name of organization / entity
Rasht University of Medical Sciences
Full name of responsible person
Mohammadreza Naghipour
Street address
No. 14, Farhang Ave., Talebamoli Blvd., Amol Town
City
Amol
Province
Mazandaran
Postal code
4615973556
Phone
+98 11 4428 9094
Email
kosar.valaei94@gmail.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Rasht University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic
Person responsible for general inquiries
Contact
Name of organization / entity
Rasht University of Medical Sciences
Full name of responsible person
Kosar Valaei
Position
Student
Latest degree
Master
Other areas of specialty/work
Physiology
Street address
No. 14, Farhang Ave., Talebamoli Blvd., Amol Town
City
Amol
Province
Mazandaran
Postal code
4615973556
Phone
+98 11 4428 9094
Email
kosar.valaei94@gmail.com
Person responsible for scientific inquiries
Contact
Name of organization / entity
Rasht University of Medical Sciences
Full name of responsible person
Kosar Valaei
Position
Student
Latest degree
Master
Other areas of specialty/work
Physiology
Street address
No. 14, Farhang Ave., Talebamoli Blvd., Amol Town
City
Amol
Province
Mazandaran
Postal code
4615973556
Phone
+98 11 4428 9094
Email
kosar.valaei94@gmail.com
Person responsible for updating data
Contact
Name of organization / entity
Rasht University of Medical Sciences
Full name of responsible person
Kosar Valaei
Position
Student
Latest degree
Master
Other areas of specialty/work
Physiology
Street address
No. 14, Farhang Ave., Talebamoli Blvd., Amol Town
City
Amol
Province
Mazandaran
Postal code
4615973556
Phone
+98 11 4428 9094
Email
kosar.valaei94@gmail.com
Sharing plan
Deidentified Individual Participant Data Set (IPD)
Yes - There is a plan to make this available
Study Protocol
Yes - There is a plan to make this available
Statistical Analysis Plan
Yes - There is a plan to make this available
Informed Consent Form
Yes - There is a plan to make this available
Clinical Study Report
Yes - There is a plan to make this available
Analytic Code
No - There is not a plan to make this available
Data Dictionary
No - There is not a plan to make this available
Title and more details about the data/document
After the study, information about the main outcome will be shared.
When the data will become available and for how long
6 months after publication
To whom data/document is available
Scientific researchers
Under which criteria data/document could be used
Data and results will be available for research on L-citrulline.