History
# Registration date Revision Id
2 2019-09-25, 1398/07/03 105158
1 2016-05-01, 1395/02/12 6106
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  • Protocol summary

    Objective: The aim of this study is to determine the effects of zinc supplementation on expressed levels of PPAR-γ and GLUT1 genes in newborns of women with gestational diabetes. Study design: Parallel double-blind (both patients and researchers) clinical trial. Randomization will be done by the use of computer-generated random numbers. Inclusion criteria: Patients with gestational diabetes aged 18 to 40 years. Exclusion criteria: Unwillingness to cooperate. Population and sample size: Among patients with gestational diabetes referred to Naghavi Clinic affiliated to Kashan University of Medical Sciences, 40 patients will be selected according to inclusion and eclusion criteria. Intervention: Patients will be assigned into two groups to receive zinc supplements (n=20) or placebo (n=20). Zinc supplements and placebos capsules are similar in shape and size. Fasting blood samples will be taken at baseline and 6 weeks after the intervention. At the beginning and end of the intervention: 6 weeks. Although, the duration of intervention was 6 weeks, all women were followed up until the delivery. Outcomes: Blood samples from cord will be taken to quantify expressed levels of PPAR-γ and GLUT1 genes.
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    هدف: هدف این مطالعه تعیین اثرات مکمل یاری روی بر بر سطوح بیان ژن های PPAR-γ و GLUT1 در نوزادان زنان مبتلا به دیابت بارداری است. طراحي انجام مطالعه: کارآزمایی بالینی دو سوکور (هم بیماران و هم محققان) موازی. تصادفی سازی با استفاده ازجدول اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد. معیار ورود به مطالعه: بیماران مبتلا به دیابت بارداری در محدوده سنی 18 تا 40 سال. معیار خروج از مطالعه: عدم تمایل به همکاری. حجم نمونه و جمعیت مورد مطالعه: از بین بیماران مبتلا به دیابت بارداری ارجاع شده به کلینیک نقوی وابسته به دانشگاه علوم پزشکی کاشان، 40 بیمار بر اساس معیار های ورود و خروج از مطالعه انتخاب خواهند شد. مداخله مورد مطالعه: بیماران به دو گروه برای دریافت مکمل روی (20=n) یا پلاسبو (20=n) اختصاص داده خواهند شد. کپسول های مکمل روی و پلاسبو از نظر شکل و اندازه مشابه هستند. نمونه خون ناشتا در ابتدای مطالعه و 6 هفته بعد از مداخله از بیماران گرفته خواهد شد. زمان مداخله: 6 هفته. اگرچه، طول مدت مداخله 6 هفته بود ولی همه زنان تا زمان زایمان پیگیری شدند. پيامدها: نمونه خون از بند ناف برای اندازه گیری سطوح بیان ژن های PPAR-γ و GLUT1 گرفته خواهد شد.
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    Objective: The aim of this study is to determine the effects of zinc supplementation on expressed levels of insulin receptor, PPAR-alpha and GLUT4 genes in newborns of women with gestational diabetes.
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    هدف: هدف این مطالعه تعیین اثرات مکمل یاری روی بر بر سطوح بیان ژن های انسولین رسپتور، PPAR-alpha و GLUT4 در نوزادان زنان مبتلا به دیابت بارداری است.
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    Parallel double-blind randomized controlled clinical trial.
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    تصادفي کارآزمایی بالینی دو سوکور موازی
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    50 patients with GDM among pregnant women of eligible and referred to gynecology Clinic affiliated to Kashan University of Medical Sciences, Kashan, Iran in the study will be selected.
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    50 زن باردار مبتلا به دیابت بارداری واجد شرایط و ارجاع شده به کلینیک زنان وابسته به دانشگاه علوم پزشکی کاشان، کاشان، ایران در مطالعه انتخاب خواهند شد.
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    Pregnant women aged 18-40 years diagnosed with GDM at 24-28 weeks' gestation will be included in this study. Exclusion criteria will be insulin-dependent diabetes, smoking and history of kidney stones.
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    زنان باردار 40-18 ساله مبتلا به دیابت بارداری (GDM) در هفته 28-24 بارداری، در این مطالعه وارد خواهند شد. معیارهای خروج از مطالعه شامل دیابت وابسته به انسولین ، سیگار کشیدن و سابقه سنگ کلیه خواهد بود.
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    Patients will be assigned to receive either zinc supplements (intervention group: n=25) or placebo (control group: n=25).Although the duration of intervention was 6 weeks, all women were followed up until the delivery.
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    بیماران برای دریافت ترکیب روی (گروه مداخله: n=25) یا پلاسبو (گروه کنترل n=25) تقسیم خواهند شد.زمان مداخله: 6 هفته. اگرچه، طول مدت مداخله 6 هفته بود ولی همه زنان تا زمان زایمان پیگیری شدند.
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    Outcomes: Blood samples from cord will be taken to quantify expressed levels of insulin receptor (primary outcome), PPAR-alpha and GLUT4 genes (secondary outcomes).
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    پيامدها: نمونه خون از بند ناف برای اندازه گیری سطوح بیان ژن های انسولین رسپتور (پیامد اولیه)، PPAR-alpha و GLUT4 (پیامدهای ثانویه) گرفته خواهد شد.
  • General information

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    2016-03-15, 1394/12/25
    2016-03-20, 1395/01/01
    Clinical trial of the effect of zinc supplementation compared with the placebo on expressed levels of PPAR-γ and GLUT1 genes in newborns of women with gestational diabetes
    Clinical trial of the effect of zinc supplementation compared with the placebo on expressed levels of insulin receptor, PPAR-alpha and GLUT4 genes in newborns of women with gestational diabetes
    کارآزمایی بالینی اثر مکمل یاری روی در مقایسه با پلاسبو بر سطوح بیان ژن های PPAR-γ و GLUT1 در نوزادان زنان مبتلا به دیابت بارداری
    کارآزمایی بالینی اثر مکمل یاری روی در مقایسه با پلاسبو بر سطوح بیان ژن های انسولین رسپتور، PPAR-alpha و GLUT4 در نوزادان زنان مبتلا به دیابت بارداری
    Patients with gestational diabetes aged 18 to 40 years
    بیماران مبتلا به مبتلا به دیابت بارداری در محدوده سنی 18 تا 40 سال
    Insulin-dependent diabetes
    Smoking
    History of kidney stones
    دیابت وابسته به انسولین
    سیگار کشیدن
    سابقه سنگ کلیه
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    At study baseline and after stratification for pre-intervention BMI and age, subjects will be randomly divided into two groups to take either supplements (n = 25) or placebo (n = 25). Randomization will be done by the use of computer-generated random numbers.
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    در ابتدای مطالعه و بعد از طبقه بندی بر اساس مقادیر پایه نمایه توده بدنی و سن، افراد به طور تصادفی به دو گروه برای دریافت مکمل (n = 25) و پلاسبو (n =25) تخصیص داده می شوند. تصادفی سازی با استفاده از جدول اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد.
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    Randomization and allocation will be concealed from the researchers and participants until the final analyses are completed. Another person at the gynecology clinics affiliated to Kashan University of Medical Sciences, who is not involved in the trial and not aware of random sequences, will be assigned the participants to the numbered bottles of capsules.
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    تصادفی سازی و تخصیص تصادفی از محققان و شرکت کنندگان در مطالعه تا زمان تکمیل آنالیز آماری پنهان خواهد ماند. فرد دیگری در کلینیک زنان وابسته به دانشگاه علوم پزشکی کاشان که در این کارآزمایی بالینی نقشی ندارد و از تخصیص تصادفی آگاه نیست، به شرکت کنندگان در مطالعه، بطری های شماره گذاری شده کپسول ها را تخصیص خواهد داد.
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  • Primary outcomes

    #1
    Expressed levels of PPAR-γ gene
    Expressed levels of insulin receptor 1 gene
    سطوح بیان ژن PPAR-γ
    سطوح بیان ژن انسولین رسپتور 1
    #2
    Expressed levels of GLUT1 gene
    Expressed levels of insulin receptor 2 gene
    سطوح بیان ژن GLUT1
    سطوح بیان ژن انسولین رسپتور 2
  • Secondary outcomes

    #1
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    Gene expression of PPAR-alpha
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    بیان ژن PPAR-alpha
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    Delivery time
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    زمان زایمان
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    PCR
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    PCR
    #2
    empty
    Gene expression of GLUT4
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    بیان ژن GLUT4
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    Delivery time
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    زمان زایمان
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    PCR
    empty
    PCR
    #3
    empty
    Gene expression of TGFB
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    بیان ژن TGFB
    empty
    Delivery time
    empty
    زمان زایمان
    empty
    PCR
    empty
    PCR
  • Recruitment centers

    #1
    Name of recruitment center - English: Naghavi Clinic
    Name of recruitment center - Persian: کلینیک نقوی
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Street address - English: Shahid Rajaee Avenue, Kashan
    Street address - Persian: کاشان، خیابان شهید رجایی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:
    Name of recruitment center - English: Naghavi Clinic
    Name of recruitment center - Persian: کلینیک نقوی
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Street address - English: Shahid Rajaee Avenue, Kashan
    Street address - Persian: کاشان، خیابان شهید رجایی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Country: Iran (Islamic Republic of)
    Postal code: 81151-87159
    Phone: +98 31 5562 0608
    Fax:
    Email: asemi_r@yahoo.com
    Web page address:
  • Sponsors / Funding sources

    #1
    contact.organization_id:
    Name of organization / entity - English: Vice chancellor for research, Kashan University of Medical Sciences
    Name of organization / entity - Persian: معاونت پژوهشی دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Gholamali Hamidi
    Full name of responsible person - Persian: غلامعلی حمیدی
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Vice chancellor for research, Kashan University of Medical Sciences
    Name of organization / entity - Persian: معاونت پژوهشی دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Gholamali Hamidi
    Full name of responsible person - Persian: غلامعلی حمیدی
    Street address - English: Bolvare Ghotbe Ravandi, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Country: Iran (Islamic Republic of)
    Postal code: 81151-87159
    Phone: +98 31 5546 3378
    Fax:
    Email: research@kaums.ac.ir
    Web page address:
    empty
    Public
    empty
    Domestic
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    Academic
  • Person responsible for general inquiries

    contact.organization_id:
    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English:
    Full name of responsible person - Persian:
    Position - English:
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    Latest degree:
    Area of specialty/work:
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    contact.provinces_available:
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    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: Associate professor
    Position - Persian: دانشیار
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Bolvare Ghotbe Ravandi, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 81151-87159
    Phone: +98 31 5546 3378
    Mobile: 00
    Fax:
    Email: asemi_r@yahoo.com
    Web page address:
  • Person responsible for scientific inquiries

    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکترای تغذیه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir; asemi_r@yahoo.com
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکترای تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 81151-87159
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_r@yahoo.com
    Web page address:
  • Person responsible for updating data

    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir; asemi_r@yahoo.com
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Bolvare Ghotbe Ravandi, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 81151-87159
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_r@yahoo.com
    Web page address:
  • Sharing plan

    undecided
    undecided
    undecided
    undecided
    undecided
    undecided
    undecided
    empty
    Undecided - It is not yet known if there will be a plan to make this available.
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    هنوز تصمیم نگرفته‌ام - برنامه انتشار آن هنوز مشخص نیست.

Protocol summary

Study aim
Objective: The aim of this study is to determine the effects of zinc supplementation on expressed levels of insulin receptor, PPAR-alpha and GLUT4 genes in newborns of women with gestational diabetes.
Design
Parallel double-blind randomized controlled clinical trial.
Settings and conduct
50 patients with GDM among pregnant women of eligible and referred to gynecology Clinic affiliated to Kashan University of Medical Sciences, Kashan, Iran in the study will be selected.
Participants/Inclusion and exclusion criteria
Pregnant women aged 18-40 years diagnosed with GDM at 24-28 weeks' gestation will be included in this study. Exclusion criteria will be insulin-dependent diabetes, smoking and history of kidney stones.
Intervention groups
Patients will be assigned to receive either zinc supplements (intervention group: n=25) or placebo (control group: n=25).Although the duration of intervention was 6 weeks, all women were followed up until the delivery.
Main outcome variables
Outcomes: Blood samples from cord will be taken to quantify expressed levels of insulin receptor (primary outcome), PPAR-alpha and GLUT4 genes (secondary outcomes).

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT201604195623N76
Registration date: 2016-05-01, 1395/02/12
Registration timing: retrospective

Last update: 2019-09-26, 1398/07/04
Update count: 1
Registration date
2016-05-01, 1395/02/12
Registrant information
Name
Zatollah Asemi
Name of organization / entity
Kashan University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 36 1534 3570
Email address
asemi_z@kaums.ac.ir
Recruitment status
Recruitment complete
Funding source
Kashan University of Medical Sciences
Expected recruitment start date
2016-02-20, 1394/12/01
Expected recruitment end date
2016-03-20, 1395/01/01
Actual recruitment start date
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Actual recruitment end date
empty
Trial completion date
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Scientific title
Clinical trial of the effect of zinc supplementation compared with the placebo on expressed levels of insulin receptor, PPAR-alpha and GLUT4 genes in newborns of women with gestational diabetes
Public title
Effect of supplementation in treatment of women with gestational diabetes
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Patients with gestational diabetes aged 18 to 40 years
Exclusion criteria:
Insulin-dependent diabetes Smoking History of kidney stones
Age
From 18 years old to 40 years old
Gender
Female
Phase
3
Groups that have been masked
  • Participant
  • Investigator
  • Outcome assessor
Sample size
Target sample size: 50
Randomization (investigator's opinion)
Randomized
Randomization description
At study baseline and after stratification for pre-intervention BMI and age, subjects will be randomly divided into two groups to take either supplements (n = 25) or placebo (n = 25). Randomization will be done by the use of computer-generated random numbers.
Blinding (investigator's opinion)
Double blinded
Blinding description
Randomization and allocation will be concealed from the researchers and participants until the final analyses are completed. Another person at the gynecology clinics affiliated to Kashan University of Medical Sciences, who is not involved in the trial and not aware of random sequences, will be assigned the participants to the numbered bottles of capsules.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

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Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Kashan University of Medical Sciences
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
81151-87159
Approval date
2016-03-09, 1394/12/19
Ethics committee reference number
IR.Kaums.REC.1394.163

Health conditions studied

1

Description of health condition studied
Gestational diabetes
ICD-10 code
O24.9
ICD-10 code description
Diabetes mellitus in pregnancy, unspecified

Primary outcomes

1

Description
Expressed levels of insulin receptor 1 gene
Timepoint
Dlivery time
Method of measurement
PCR

2

Description
Expressed levels of insulin receptor 2 gene
Timepoint
Dlivery time
Method of measurement
PCR

Secondary outcomes

1

Description
Gene expression of PPAR-alpha
Timepoint
Delivery time
Method of measurement
PCR

2

Description
Gene expression of GLUT4
Timepoint
Delivery time
Method of measurement
PCR

3

Description
Gene expression of TGFB
Timepoint
Delivery time
Method of measurement
PCR

Intervention groups

1

Description
Intervention group: Zinc supplements, 30 mg, daily, for 6 weeks orally.
Category
Treatment - Drugs

2

Description
Control group: Placebo capsule, daily for 6 weeks orally.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Naghavi Clinic
Full name of responsible person
Zatollah Asemi
Street address
Shahid Rajaee Avenue, Kashan
City
Kashan
Province
Isfehan
Postal code
81151-87159
Phone
+98 31 5562 0608
Email
asemi_r@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Vice chancellor for research, Kashan University of Medical Sciences
Full name of responsible person
Gholamali Hamidi
Street address
Bolvare Ghotbe Ravandi, Kashan
City
Kashan
Province
Isfehan
Postal code
81151-87159
Phone
+98 31 5546 3378
Email
research@kaums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Vice chancellor for research, Kashan University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Bolvare Ghotbe Ravandi, Kashan
City
Kashan
Province
Isfehan
Postal code
81151-87159
Phone
+98 31 5546 3378
Email
asemi_r@yahoo.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
81151-87159
Phone
+98 31 5546 3378
Fax
Email
asemi_r@yahoo.com
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Bolvare Ghotbe Ravandi, Kashan
City
Kashan
Province
Isfehan
Postal code
81151-87159
Phone
+98 31 5546 3378
Fax
Email
asemi_r@yahoo.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
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