History
# Registration date Revision Id
2 2019-09-22, 1398/06/31 104606
1 2016-12-04, 1395/09/14 6125
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  • Protocol summary

    Objective: The aim of this study is to determine the effects of selenium supplementation on gene expression involved in inflammation signaling pathway in patients with gestational diabetes. Study design: Parallel double-blind (both patients and researchers) randomized controlled clinical trial. Random assignment will be done by the use of computer-generated random numbers. Inclusion criteria: Patients with gestational diabetes and aged 18 to 40 years will be included in this study. Exclusion criteria: Unwillingness to cooperate will be excluded in the study. Population and sample size: 40 patients with gestational diabetes of eligible and referred to Kosar Clinic affiliated to Arak University of Medical Sciences, Arak, Iran in the study will be selected. Intervention: Patients will be assigned to receive either selenium (n=20) or placebo (n=20). Selenium and placebos capsules are similar in shape and size. Fasting blood samples will be taken at baseline and 6 weeks after the intervention. At the beginning and end of the intervention: 6 weeks. Outcomes: Gene expression involved in TNF-α (primary outcomes), and other inflammatory factors (secondry outcomes) signaling pathway will be quantified at study baseline and end-of-trial.
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    هدف: هدف این مطالعه تعیین اثرات مکمل یاری سلنیوم بر بیان ژن دخیل در مسیر سیگنالینگ التهاب در بیماران مبتلا به دیابت بارداری است. طراحي انجام مطالعه: تصادفي کارآزمایی بالینی دو سوکور (هم بیماران و هم محققان) موازی. تصادفی سازی با استفاده از اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد. معیار ورود به مطالعه: بیماران مبتلا به دیابت بارداری در محدوده سنی 18 تا 40 سال وارد مطالعه خواهند شد. معیار خروج از مطالعه: عدم تمایل به همکاری از مطالعه خارج خواهند شد. حجم نمونه و جمعیت مورد مطالعه: 40 بیمار مبتلا به دیابت بارداری واجد شرایط و ارجاع شده به کلینیک کوثر وابسته به دانشگاه علوم پزشکی اراک، اراک، ایران در مطالعه انتخاب خواهند شد. مداخله مورد مطالعه: بیماران برای دریافت یا مکمل سلنیوم (20=n) یا پلاسبو (20=n) تقسیم خواهند شد. کپسول های مکمل سلنیوم و پلاسبو از نظر شکل و اندازه مشابه هستند. نمونه خون ناشتا در ابتدای مطالعه و 6 هفته بعد از مداخله از بیماران گرفته خواهد شد. زمان مداخله: 6 هفته. پيامدها: بیان ژن دخیل در مسیر سیگنالینگ TNF-α (پیامدهای اولیه) و سایر فاکتورهای التهابی (پیامدهای ثانویه) در ابتدا و انتهای مداخله اندازه گیری خواهد شد.
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    The aim of this study is to determine the effects of selenium supplementation on gene expression involved in inflammation signaling pathway in patients with gestational diabetes.
    empty
    هدف این مطالعه تعیین اثرات مکمل یاری سلنیوم بر بیان ژن دخیل در مسیر سیگنالینگ التهاب در بیماران مبتلا به دیابت بارداری است.
    empty
    Study design: Parallel double-blind (both patients and researchers) randomized controlled clinical trial. Random assignment will be done by the use of computer-generated random numbers.
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    طراحي انجام مطالعه: تصادفي کارآزمایی بالینی دو سوکور (هم بیماران و هم محققان) موازی. تصادفی سازی با استفاده از اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد.
    empty
    Population and sample size: 40 patients with gestational diabetes of eligible and referred to Kosar Clinic affiliated to Arak University of Medical Sciences, Arak, Iran in the study will be selected.
    empty
    حجم نمونه و جمعیت مورد مطالعه: 40 بیمار مبتلا به دیابت بارداری واجد شرایط و ارجاع شده به کلینیک کوثر وابسته به دانشگاه علوم پزشکی اراک، اراک، ایران در مطالعه انتخاب خواهند شد.
    empty
    Inclusion criteria: Patients with gestational diabetes and aged 18 to 40 years will be included in this study. Exclusion criteria: Taking selenium supplements before the intervention, needing insulin therapy, placenta abruption, pre-eclampsia, eclampsia, hypo and hyperthyroidism and Smokers
    empty
    معیار ورود به مطالعه: بیماران مبتلا به دیابت بارداری در محدوده سنی 18 تا 40 سال وارد مطالعه خواهند شد. معیار خروج از مطالعه: مصرف مکمل های سلنیوم قبل از مداخله، نیاز به انسولین درمانی، سقط جنین، پره اکلامپسی، اکلامپسی، کم کاری و پرکاری تیروئید و افراد سیگاری
    empty
    Intervention: Patients will be assigned to receive either selenium (n=20) or placebo (n=20). Selenium and placebos capsules are similar in shape and size.
    empty
    مداخله مورد مطالعه: بیماران برای دریافت یا مکمل سلنیوم (20=n) یا پلاسبو (20=n) تقسیم خواهند شد. کپسول های مکمل سلنیوم و پلاسبو از نظر شکل و اندازه مشابه هستند.
    empty
    Outcomes: Gene expression involved in TNF-α (primary outcomes), and other inflammatory factors (secondry outcomes) signaling pathway will be quantified at study baseline and end-of-trial.
    empty
    پيامدها: بیان ژن دخیل در مسیر سیگنالینگ TNF-α (پیامدهای اولیه) و سایر فاکتورهای التهابی (پیامدهای ثانویه) در ابتدا و انتهای مداخله اندازه گیری خواهد شد.
  • General information

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    2016-11-30, 1395/09/10
    2016-12-10, 1395/09/20
    Patients with gestational diabetes
    aged 18 to 40 years
    بیماران مبتلا به دیابت بارداری
    در محدوده سنی 18 تا 40 سال
    Taking selenium supplements before the intervention
    Needing insulin therapy
    Placenta abruption
    Pre-eclampsia
    Eclampsia
    Hypo and hyperthyroidism
    Smokers
    مصرف مکمل های سلنیوم قبل از مداخله
    نیاز به انسولین درمانی
    سقط جنین
    پره اکلامپسی
    اکلامپسی
    کم کاری و پرکاری تیروئید
    افراد سیگاری
    Random assignment will be done by the use of computer-generated random numbers.
    تصادفی سازی با استفاده از اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد.
    empty
    Randomization will be done by the use of computer-generated random numbers.
    empty
    تصادفی سازی با استفاده ازجدول اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد.
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    Participants, investigators or the assessors of the outcomes are unaware of the study groups.
    empty
    شرکت کنندگان و هم محققان یا ارزیابان پیامد از تخصیص گروههای مطالعه بی اطلاعند.
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  • Secondary outcomes

    #1
    empty
    VEGF
    empty
    VEGF
    empty
    At the beginning of the study and after 6 weeks of intervention
    empty
    در ابتدای مطالعه و 6 هفته بعد از مداخله
    empty
    PCR-RT
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    PCR-RT
  • Intervention groups

    #1
    Intervention group: 200 µg selenium (21 century, New York, USA), once a day, for 6 weeks orally.
    Intervention group: 200 µg selenium (Nature Made, California, USA), once a day, for 6 weeks orally.
    گروه مداخله: 200 میکروگرم سلنیوم (قرن 21، نیویورک، آمریکا)، یک بار در روز، به صورت خوراکی برای 6 هفته.
    گروه مداخله: 200 میکروگرم سلنیوم (نیچر، کالیفرنیا، آمریکا)، یک بار در روز، به صورت خوراکی برای 6 هفته.
  • Recruitment centers

    #1
    Name of recruitment center - English: Kosar Clinic
    Name of recruitment center - Persian: کلینیک کوثر
    Full name of responsible person - English: Mehri Jamilian
    Full name of responsible person - Persian: مهری جمیلیان
    Street address - English: Emam Khomeyni Avenue, Arak
    Street address - Persian: اراک، خیابان امام خمینی
    City - English: Arak
    City - Persian: اراک
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:
    Name of recruitment center - English: Kosar Clinic
    Name of recruitment center - Persian: کلینیک کوثر
    Full name of responsible person - English: Mehri Jamilian
    Full name of responsible person - Persian: مهری جمیلیان
    Street address - English: Emam Khomeyni Avenue, Arak
    Street address - Persian: اراک، خیابان امام خمینی
    City - English: Arak
    City - Persian: اراک
    Province: Markazi
    Country: Iran (Islamic Republic of)
    Postal code: 3848176941
    Phone: +98 84 3223 3823
    Fax:
    Email: research@arakmu.ac.ir
    Web page address:
  • Sponsors / Funding sources

    #1
    contact.organization_id:
    Name of organization / entity - English: Vice chancellor for research, Arak University of Medical Sciences
    Name of organization / entity - Persian: معاونت پژوهشی دانشگاه علوم پزشکی اراک
    Full name of responsible person - English: Mohammad Rafiee
    Full name of responsible person - Persian: محمد رفیعی
    Street address - English: Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
    Street address - Persian: اراك، معاونت پژوهشی دانشگاه علوم پزشکی اراک، خیابان سردشت، معاونت پژوهشی
    City - English: Arak
    City - Persian: اراک
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Vice chancellor for research, Arak University of Medical Sciences
    Name of organization / entity - Persian: معاونت پژوهشی دانشگاه علوم پزشکی اراک
    Full name of responsible person - English: Mohammad Rafiee
    Full name of responsible person - Persian: محمد رفیعی
    Street address - English: Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
    Street address - Persian: اراك، معاونت پژوهشی دانشگاه علوم پزشکی اراک، خیابان سردشت، معاونت پژوهشی
    City - English: Arak
    City - Persian: اراک
    Province: Markazi
    Country: Iran (Islamic Republic of)
    Postal code: 3848176941
    Phone: +98 86 3417 3639
    Fax:
    Email: research@arakmu.ac.ir
    Web page address:
    empty
    Public
    empty
    Domestic
    empty
    Academic
  • Person responsible for general inquiries

    contact.organization_id:
    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English:
    Full name of responsible person - Persian:
    Position - English:
    Position - Persian:
    Latest degree:
    Area of specialty/work:
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    Street address - English:
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    Province - Persian:
    contact.provinces_available:
    Country:
    Postal code:
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    Mobile:
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    Web page address:

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: Nutritionist
    Position - Persian: متخصص تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 81151-87159
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir
    Web page address:
  • Person responsible for scientific inquiries

    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکترای تغذیه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir; asemi_r@yahoo.com
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکترای تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 81151-87159
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir
    Web page address:
  • Person responsible for updating data

    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذ ات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکترای تغذیه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir; asemi_r@yahoo.com
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذ ات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکترای تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 81151-87159
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir
    Web page address:
  • Sharing plan

    undecided
    undecided
    undecided
    undecided
    undecided
    undecided
    undecided
    empty
    Undecided - It is not yet known if there will be a plan to make this available.
    empty
    هنوز تصمیم نگرفته‌ام - برنامه انتشار آن هنوز مشخص نیست.

Protocol summary

Study aim
The aim of this study is to determine the effects of selenium supplementation on gene expression involved in inflammation signaling pathway in patients with gestational diabetes.
Design
Study design: Parallel double-blind (both patients and researchers) randomized controlled clinical trial. Random assignment will be done by the use of computer-generated random numbers.
Settings and conduct
Population and sample size: 40 patients with gestational diabetes of eligible and referred to Kosar Clinic affiliated to Arak University of Medical Sciences, Arak, Iran in the study will be selected.
Participants/Inclusion and exclusion criteria
Inclusion criteria: Patients with gestational diabetes and aged 18 to 40 years will be included in this study. Exclusion criteria: Taking selenium supplements before the intervention, needing insulin therapy, placenta abruption, pre-eclampsia, eclampsia, hypo and hyperthyroidism and Smokers
Intervention groups
Intervention: Patients will be assigned to receive either selenium (n=20) or placebo (n=20). Selenium and placebos capsules are similar in shape and size.
Main outcome variables
Outcomes: Gene expression involved in TNF-α (primary outcomes), and other inflammatory factors (secondry outcomes) signaling pathway will be quantified at study baseline and end-of-trial.

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT201612045623N95
Registration date: 2016-12-04, 1395/09/14
Registration timing: registered_while_recruiting

Last update: 2019-09-22, 1398/06/31
Update count: 1
Registration date
2016-12-04, 1395/09/14
Registrant information
Name
Zatollah Asemi
Name of organization / entity
Kashan University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 36 1534 3570
Email address
asemi_z@kaums.ac.ir
Recruitment status
Recruitment complete
Funding source
Vice chancellor for research, Arak University of Medical Sciences
Expected recruitment start date
2016-11-15, 1395/08/25
Expected recruitment end date
2016-12-10, 1395/09/20
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Clinical trial of the effect of selenium supplementation compared with the placebo on gene expression involved in inflammation signaling pathway in patients with gestational diabetes
Public title
Effect of supplementation in treatment of patients with gestational diabetes
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Patients with gestational diabetes aged 18 to 40 years
Exclusion criteria:
Taking selenium supplements before the intervention Needing insulin therapy Placenta abruption Pre-eclampsia Eclampsia Hypo and hyperthyroidism Smokers
Age
From 18 years old to 40 years old
Gender
Female
Phase
3
Groups that have been masked
  • Participant
  • Investigator
  • Outcome assessor
Sample size
Target sample size: 40
Randomization (investigator's opinion)
Randomized
Randomization description
Randomization will be done by the use of computer-generated random numbers.
Blinding (investigator's opinion)
Double blinded
Blinding description
Participants, investigators or the assessors of the outcomes are unaware of the study groups.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Arak University of Medical Sciences
Street address
Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
City
Arak
Province
Markazi
Postal code
3848176941
Approval date
2016-11-14, 1395/08/24
Ethics committee reference number
IR.ARAKMU.REC.1395.308

Health conditions studied

1

Description of health condition studied
Gestational diabetes
ICD-10 code
E28.2
ICD-10 code description
Diabetes mellitus in pregnancy, unspecified

Primary outcomes

1

Description
TNF-α
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
PCR-RT

Secondary outcomes

1

Description
IL-1
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
PCR-RT

2

Description
IL-8
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
PCR-RT

3

Description
TGF-B
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
PCR-RT

4

Description
VEGF
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
PCR-RT

Intervention groups

1

Description
Intervention group: 200 µg selenium (Nature Made, California, USA), once a day, for 6 weeks orally.
Category
Treatment - Drugs

2

Description
Control group: Placebo (Barij Essence, Kashan, Iran), once a day, for 6 weeks orally.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Kosar Clinic
Full name of responsible person
Mehri Jamilian
Street address
Emam Khomeyni Avenue, Arak
City
Arak
Province
Markazi
Postal code
3848176941
Phone
+98 84 3223 3823
Email
research@arakmu.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Vice chancellor for research, Arak University of Medical Sciences
Full name of responsible person
Mohammad Rafiee
Street address
Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
City
Arak
Province
Markazi
Postal code
3848176941
Phone
+98 86 3417 3639
Email
research@arakmu.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Vice chancellor for research, Arak University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
Nutritionist
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
81151-87159
Phone
+98 31 5546 3378
Email
asemi_z@kaums.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
81151-87159
Phone
+98 31 5546 3378
Fax
Email
asemi_z@kaums.ac.ir
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
81151-87159
Phone
+98 31 5546 3378
Fax
Email
asemi_z@kaums.ac.ir
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
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