History
# Registration date Revision Id
2 2019-09-20, 1398/06/29 104793
1 2017-06-09, 1396/03/19 6149
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  • Protocol summary

    Objective: The aim of this study is to determine the effects of combined probiotic and vitamin D supplementation on gene expression related to insulin and lipid in patients of gestational diabetes. Study design: Parallel double-blind (both patients and researchers) clinical trial. Randomization will be done by the use of computer-generated random numbers. Inclusion criteria: Patients with gestational diabetes aged 18 to 40 years. Exclusion criteria: Unwillingness to cooperate. Population and sample size: Among patients with gestational diabetes referred to Kosar Clinic affiliated to Arak University of Medical Sciences, 90 patients will be selected according to inclusion and exclusion criteria. Intervention: Patients will be assigned into three groups to receive combined probiotic and vitamin D (n=30) or probiotic (n=30) or placebo (n=30). Probiotic, vitamin D and placebos capsules are similar in shape and size. Fasting blood samples will be taken at baseline and 6 weeks after the intervention. At the beginning and the end of the intervention: 6 weeks. Outcomes: Insulin resistance (primary outcomes) and lipid profiles, biomarkers of inflammation and oxidative stress (secondary outcome) will be quantified at study baseline and end-of-trial.
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    هدف: هدف این مطالعه تعیین اثرات مکمل یاری ترکیب پروبیوتیک و ویتامین D بر پروفایل های متابولیک در بیماران مبتلا به دیابت بارداری است. طراحي انجام مطالعه: کارآزمایی بالینی دو سوکور (هم بیماران و هم محققان) موازی. تصادفی سازی با استفاده ازجدول اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد. معیار ورود به مطالعه: بیماران مبتلا به دیابت بارداری در محدوده سنی 18 تا 40 سال. معیار خروج از مطالعه: عدم تمایل به همکاری. حجم نمونه و جمعیت مورد مطالعه: از بین بیماران مبتلا به دیابت بارداری ارجاع شده به کلینیک کوثر وابسته به دانشگاه علوم پزشکی اراک، 90 بیمار بر اساس معیار های ورود و خروج از مطالعه انتخاب خواهند شد. مداخله مورد مطالعه: بیماران به سه گروه برای دریافت ترکیب پروبیوتیک و ویتامین D (30=n) یا پروبیوتیک (30=n) یا پلاسبو (30=n) اختصاص داده خواهند شد. کپسول های پروبیوتیک، ویتامین D و پلاسبو از نظر شکل و اندازه مشابه هستند. نمونه خون ناشتا در ابتدای مطالعه و 6 هفته بعد از مداخله از بیماران گرفته خواهد شد. زمان مداخله: 6 هفته. پيامدها: مقاومت به انسولین (پیامدهای اولیه) و پروفایل های لیپیدی، بیومارکرهای التهابی و استرس اکسیداتیو (پیامدهای ثانویه) در ابتدا و انتهای مداخله اندازه گیری خواهد شد.
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    Objective: The aim of this study is to determine the effects of combined probiotic and vitamin D supplementation on gene expression related to insulin and lipid in patients of gestational diabetes.
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    هدف: هدف این مطالعه تعیین اثرات مکمل یاری ترکیب پروبیوتیک و ویتامین D بر پروفایل های متابولیک در بیماران مبتلا به دیابت بارداری است.
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    Study design: Parallel double-blind (both patients and researchers) clinical trial. Randomization will be done by the use of computer-generated random numbers.
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    طراحي انجام مطالعه: کارآزمایی بالینی دو سوکور (هم بیماران و هم محققان) موازی. تصادفی سازی با استفاده ازجدول اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد.
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    Among patients with gestational diabetes referred to Kosar gynecology Clinic affiliated to Arak University of Medical Sciences, 90 patients will be selected according to inclusion and exclusion criteria. Participants, investigators or the assessors of the outcomes are unaware of the study groups.
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    از بین بیماران مبتلا به دیابت بارداری ارجاع شده به کلینیک زنان کوثر وابسته به دانشگاه علوم پزشکی اراک، 90 بیمار بر اساس معیار های ورود و خروج از مطالعه انتخاب خواهند شد. شرکت کنندگان و هم محققان یا ارزیابان پیامد از تخصیص گروههای مطالعه بی اطلاعند.
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    Inclusion criteria: Patients with gestational diabetes, aged 18 to 40 years. Exclusion criteria: Taking vitamin D, probiotic and/or symbiotic during the last 3 months prior to the intervention, insulin therapy during the intervention, pre-eclampsia, eclampsia, hypo and hyperthyroidism, smokers.
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    معیار ورود به مطالعه: بیماران مبتلا به دیابت بارداری، در محدوده سنی 18 تا 40 سال. معیار خروج از مطالعه: گرفتن ویتامین دی، پروبیوتیک و/یا سیم بیوتیک طی سه ماه قبل مداخله، انسولین تراپی در طی مداخله، پری اکلامپسی، اکلامپسی، کم کاری و پرکاری تیروئید، سیگاری ها.
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    Intervention: Patients will be assigned into three groups to receive combined probiotic and vitamin D (n=30) or probiotic (n=30) or placebo (n=30). Probiotic, vitamin D and placebos capsules are similar in shape and size.
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    مداخله مورد مطالعه: بیماران به سه گروه برای دریافت ترکیب پروبیوتیک و ویتامین D (30=n) یا پروبیوتیک (30=n) یا پلاسبو (30=n) اختصاص داده خواهند شد. کپسول های پروبیوتیک، ویتامین D و پلاسبو از نظر شکل و اندازه مشابه هستند.
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    Outcomes: Insulin metabolism (primary outcomes) and lipid profiles, biomarkers of inflammation and oxidative stress (secondary outcome) will be quantified at study baseline and end-of-trial.
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    پيامدها: متابولیسم انسولین (پیامدهای اولیه) و پروفایل های لیپیدی، بیومارکرهای التهابی و استرس اکسیداتیو (پیامدهای ثانویه) در ابتدا و انتهای مداخله اندازه گیری خواهد شد.
  • General information

    1
    1
    1
    2
    3
    Patients with gestational diabetes
    Aged 18 to 40 years
    بیماران مبتلا به دیابت بارداری
    در محدوده سنی 18 تا 40 سال
    Pre-eclampsia
    Eclampsia
    Hypo and hyperthyroidism
    Smokers
    Taking vitamin D, probiotic and/or symbiotic supplements during the last 3 months prior to the intervention
    Insulin therapy during the intervention
    پری اکلامپسی
    اکلامپسی
    کم کاری و پرکاری تیروئید
    سیگاری ها
    گرفتن مکمل های ویتامین دی، پروبیوتیک و/یا سیم بیوتیک در طی 3 ماه قبل مداخله
    انسولین تراپی در طی مداخله
    Randomization will be done by the use of computer-generated random numbers.
    تصادفی سازی با استفاده ازجدول اعداد تصادفی ایجاد شده توسط کامپیوتر انجام خواهد شد.
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    To decrease potential confounding effects, all participants will have stratified randomization according to BMI (<25 and ≥25 kg/m2) and age (<30 and ≥30 y). Then, participants in each block will be randomly allocated into two treatment groups to take either supplements or placebo. Randomization will be done by the use of computer software.
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    برای کاهش اثرات مخدوش کنندگی بالقوه، همه شرکت کنندگان بر اساس نمایه توده بدنی (<25 و ≥25 کیلوگرم بر متر مربع) و سن (<30 و ≥30) تصادفی سازی لایه ای خواهند شد. سپس، افراد در هر بلوک به طور تصادفی به دو گروه برای دریافت مکمل یاری یا پلاسبو تخصیص داده می شوند. تصادفی سازی با استفاده از نرم افزار کامپیوتری انجام خواهد شد.
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    Randomization and allocation will be concealed from the researchers and participants until the final analyses are completed.
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    تصادفی سازی و تخصیص تصادفی از محققان و شرکت کنندگان در مطالعه تا زمان تکمیل آنالیز آماری پنهان خواهد ماند.
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  • Secondary outcomes

    #1
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    VLDL
    empty
    کلسترول VLDL
    empty
    At the beginning of the study and after 6 weeks of intervention
    empty
    در ابتدای مطالعه و 6 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #2
    empty
    LDL
    empty
    کلسترول LDL
    empty
    At the beginning of the study and after 6 weeks of intervention
    empty
    در ابتدای مطالعه و 6 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #3
    empty
    hs-CRP
    empty
    پروتئین واکنشگر C با حساسیت بالا
    empty
    At the beginning of the study and after 6 weeks of intervention
    empty
    در ابتدای مطالعه و 6 هفته بعد از مداخله
    empty
    Elisa kit
    empty
    کیت الایزا
    #4
    empty
    Malondialdehyde
    empty
    مالون دی آلدئید
    empty
    At the beginning of the study and after 6 weeks of intervention
    empty
    در ابتدای مطالعه و 6 هفته بعد از مداخله
    empty
    Spectrophotometry
    empty
    اسپکتروفتومتری
    #5
    empty
    Nitric oxide
    empty
    نیتریک اکساید
    empty
    At the beginning of the study and after 6 weeks of intervention
    empty
    در ابتدای مطالعه و 6 هفته بعد از مداخله
    empty
    Spectrophotometry
    empty
    اسپکتروفتومتری
    #6
    empty
    Fasting plasma glucose
    empty
    قند خون ناشتا
    empty
    At the beginning of the study and after 6 weeks of intervention
    empty
    در ابتدای مطالعه و 6 هفته بعد از مداخله
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #7
    empty
    Newborn's head circumference
    empty
    دور سر نوزادان
    empty
    Delivery time
    empty
    زمان زایمان
    empty
    Tape
    empty
    متر نواری
    #8
    empty
    Polyhydramnios
    empty
    پلی هیدروآمونیوس
    empty
    End-of-trial
    empty
    انتهای مداخله
    empty
    Sonographic
    empty
    سونوگرافی
    #9
    empty
    Newborn's bilirubin
    empty
    بیلی روبین نوزادان تازه متولد شده
    empty
    Delivery time
    empty
    زمان زایمان
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #10
    empty
    Apgar score
    empty
    عدد آپگار
    empty
    Delivery time
    empty
    زمان زایمان
    empty
    Clinical observation
    empty
    مشاهده بالینی
    #11
    empty
    Newborns' hypoglycemia
    empty
    هیپوگلیسمی نوزادان تازه متولد شده
    empty
    After delivery
    empty
    بعد زایمان
    empty
    Enzymatic kit
    empty
    کیت آنزیمی
    #12
    empty
    Preterm delivery
    empty
    زایمان زودرس
    empty
    After delivery
    empty
    بعد زایمان
    empty
    Medical record
    empty
    پرونده پزشکی
    #13
    empty
    Maternal pre-eclampsia
    empty
    پری اکلامپسی مادران باردار
    empty
    After delivery
    empty
    بعد زایمان
    empty
    Medical record
    empty
    پرونده پزشکی
    #14
    empty
    Maternal hospitalization
    empty
    بستری شدن مادران
    empty
    After delivery
    empty
    بعد زایمان
    empty
    Medical record
    empty
    پرونده پزشکی
    #15
    empty
    Newborns' hospitalization
    empty
    بستری شدن نوزادان تازه متولد شده
    empty
    After delivery
    empty
    بعد زایمان
    empty
    Medical record
    empty
    پرونده پزشکی
    #16
    empty
    Newborns' length
    empty
    قد نوزادان تازه متولد شده
    empty
    After delivery
    empty
    بعد زایمان
    empty
    Tape
    empty
    متر نواری
  • Recruitment centers

    #1
    Name of recruitment center - English: Kosar Clinic
    Name of recruitment center - Persian: کلینیک کوثر
    Full name of responsible person - English: Mehri Jamilian
    Full name of responsible person - Persian: مهری جمیلیان
    Street address - English: Emam Khomeyni Avenue, Arak
    Street address - Persian: اراک، خیابان امام خمینی
    City - English: Arak
    City - Persian: اراک
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:
    Name of recruitment center - English: Kosar gynecology Clinic
    Name of recruitment center - Persian: کلینیک زنان کوثر
    Full name of responsible person - English: Mehri Jamilian
    Full name of responsible person - Persian: مهری جمیلیان
    Street address - English: Emam Khomeyni Avenue, Arak
    Street address - Persian: اراک، خیابان امام خمینی
    City - English: Arak
    City - Persian: اراک
    Province: Markazi
    Country: Iran (Islamic Republic of)
    Postal code: 3814113634
    Phone: +98 86 3223 3823
    Fax:
    Email: jamilian.mehri@gmail.com
    Web page address:
  • Sponsors / Funding sources

    #1
    contact.organization_id:
    Name of organization / entity - English: Vice chancellor for research, Arak University of Medical Sciences
    Name of organization / entity - Persian: معاونت پژوهشی دانشگاه علوم پزشکی اراک
    Full name of responsible person - English: Mohammad Rafiee
    Full name of responsible person - Persian: محمد رفیعی
    Street address - English: Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
    Street address - Persian: اراك، معاونت پژوهشی دانشگاه علوم پزشکی اراک، خیابان سردشت، معاونت پژوهشی
    City - English: Arak
    City - Persian: اراک
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Vice chancellor for research, Arak University of Medical Sciences
    Name of organization / entity - Persian: معاونت پژوهشی دانشگاه علوم پزشکی اراک
    Full name of responsible person - English: Mohammad Rafiee
    Full name of responsible person - Persian: محمد رفیعی
    Street address - English: Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
    Street address - Persian: اراك، معاونت پژوهشی دانشگاه علوم پزشکی اراک، خیابان سردشت، معاونت پژوهشی
    City - English: Arak
    City - Persian: اراک
    Province: Markazi
    Country: Iran (Islamic Republic of)
    Postal code: 3814113634
    Phone: +98 86 3223 3823
    Fax:
    Email: rafiee-m@aums.ac.ir
    Web page address:
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    Public
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    Domestic
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    Academic
  • Person responsible for general inquiries

    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone: +98 31 5546 3378
    Mobile: 00
    Fax:
    Email: asemi_z@kaums.ac.ir; asemi_r@yahoo.com
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 1771844351
    Phone: +98 31 5546 3378
    Mobile: 00
    Fax:
    Email: asemi_r@yahoo.com
    Web page address:
  • Person responsible for scientific inquiries

    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone: +98 31 5546 3378
    Mobile: 00
    Fax:
    Email: asemi_z@kaums.ac.ir; asemi_r@yahoo.com
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 1771844351
    Phone: +98 31 5546 3378
    Mobile: 00
    Fax:
    Email: asemi_r@yahoo.com
    Web page address:
  • Person responsible for updating data

    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone: +98 31 5546 3378
    Mobile: 00
    Fax:
    Email: asemi_z@kaums.ac.ir; asemi_r@yahoo.com
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 1771844351
    Phone: +98 31 5546 3378
    Mobile: 00
    Fax:
    Email: asemi_r@yahoo.com
    Web page address:
  • Sharing plan

    undecided
    undecided
    undecided
    undecided
    undecided
    undecided
    undecided
    empty
    Undecided - It is not yet known if there will be a plan to make this available.
    empty
    هنوز تصمیم نگرفته‌ام - برنامه انتشار آن هنوز مشخص نیست.

Protocol summary

Study aim
Objective: The aim of this study is to determine the effects of combined probiotic and vitamin D supplementation on gene expression related to insulin and lipid in patients of gestational diabetes.
Design
Study design: Parallel double-blind (both patients and researchers) clinical trial. Randomization will be done by the use of computer-generated random numbers.
Settings and conduct
Among patients with gestational diabetes referred to Kosar gynecology Clinic affiliated to Arak University of Medical Sciences, 90 patients will be selected according to inclusion and exclusion criteria. Participants, investigators or the assessors of the outcomes are unaware of the study groups.
Participants/Inclusion and exclusion criteria
Inclusion criteria: Patients with gestational diabetes, aged 18 to 40 years. Exclusion criteria: Taking vitamin D, probiotic and/or symbiotic during the last 3 months prior to the intervention, insulin therapy during the intervention, pre-eclampsia, eclampsia, hypo and hyperthyroidism, smokers.
Intervention groups
Intervention: Patients will be assigned into three groups to receive combined probiotic and vitamin D (n=30) or probiotic (n=30) or placebo (n=30). Probiotic, vitamin D and placebos capsules are similar in shape and size.
Main outcome variables
Outcomes: Insulin metabolism (primary outcomes) and lipid profiles, biomarkers of inflammation and oxidative stress (secondary outcome) will be quantified at study baseline and end-of-trial.

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT201706075623N119
Registration date: 2017-06-09, 1396/03/19
Registration timing: registered_while_recruiting

Last update: 2019-09-23, 1398/07/01
Update count: 1
Registration date
2017-06-09, 1396/03/19
Registrant information
Name
Zatollah Asemi
Name of organization / entity
Kashan University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 36 1534 3570
Email address
asemi_z@kaums.ac.ir
Recruitment status
Recruitment complete
Funding source
Vice chancellor for research, Arak University of Medical Sciences
Expected recruitment start date
2017-05-16, 1396/02/26
Expected recruitment end date
2017-07-17, 1396/04/26
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Clinical trial of the effect of combined probiotic and vitamin D supplementation compared with the placebo on metabolic profiles in women with gestational diabetes
Public title
Effect of supplementation in treatment of women with gestational diabetes
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Patients with gestational diabetes Aged 18 to 40 years
Exclusion criteria:
Pre-eclampsia Eclampsia Hypo and hyperthyroidism Smokers Taking vitamin D, probiotic and/or symbiotic supplements during the last 3 months prior to the intervention Insulin therapy during the intervention
Age
From 18 years old to 40 years old
Gender
Female
Phase
3
Groups that have been masked
  • Participant
  • Investigator
  • Outcome assessor
Sample size
Target sample size: 90
Randomization (investigator's opinion)
Randomized
Randomization description
To decrease potential confounding effects, all participants will have stratified randomization according to BMI (<25 and ≥25 kg/m2) and age (<30 and ≥30 y). Then, participants in each block will be randomly allocated into two treatment groups to take either supplements or placebo. Randomization will be done by the use of computer software.
Blinding (investigator's opinion)
Double blinded
Blinding description
Randomization and allocation will be concealed from the researchers and participants until the final analyses are completed.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Arak University of Medical Sciences
Street address
Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
City
Arak
Province
Markazi
Postal code
3814113634
Approval date
2017-05-15, 1396/02/25
Ethics committee reference number
IR.ARAKMU.REC.1396.26

Health conditions studied

1

Description of health condition studied
Gestational diabetes
ICD-10 code
O24.9
ICD-10 code description
Diabetes mellitus in pregnancy, unspecified

Primary outcomes

1

Description
Insulin
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Elisa kit

2

Description
Insulin resistance
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Using HOMA formula

Secondary outcomes

1

Description
Total cholesterol
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Enzymatic kit

2

Description
Triglycerides
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Enzymatic kit

3

Description
HDL-cholesterol
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Enzymatic kit

4

Description
Total antioxidant capacity
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Spectrophotometry

5

Description
Total glutathione
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Spectrophotometry

6

Description
VLDL
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Enzymatic kit

7

Description
LDL
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Enzymatic kit

8

Description
hs-CRP
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Elisa kit

9

Description
Malondialdehyde
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Spectrophotometry

10

Description
Nitric oxide
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Spectrophotometry

11

Description
Fasting plasma glucose
Timepoint
At the beginning of the study and after 6 weeks of intervention
Method of measurement
Enzymatic kit

12

Description
Newborn's head circumference
Timepoint
Delivery time
Method of measurement
Tape

13

Description
Polyhydramnios
Timepoint
End-of-trial
Method of measurement
Sonographic

14

Description
Newborn's bilirubin
Timepoint
Delivery time
Method of measurement
Enzymatic kit

15

Description
Apgar score
Timepoint
Delivery time
Method of measurement
Clinical observation

16

Description
Newborns' hypoglycemia
Timepoint
After delivery
Method of measurement
Enzymatic kit

17

Description
Preterm delivery
Timepoint
After delivery
Method of measurement
Medical record

18

Description
Maternal pre-eclampsia
Timepoint
After delivery
Method of measurement
Medical record

19

Description
Maternal hospitalization
Timepoint
After delivery
Method of measurement
Medical record

20

Description
Newborns' hospitalization
Timepoint
After delivery
Method of measurement
Medical record

21

Description
Newborns' length
Timepoint
After delivery
Method of measurement
Tape

Intervention groups

1

Description
Intervention group: Probiotic supplements (Zist Takhmir, Tehran, Iran), 2×109 Lactobacillus acidophilus, 2×109 Bifidobacterium bifidum, 2×109 Lactobacillus reuteri, 2×109 Lactobacillus fermentum, daily, for 6 weeks orally.
Category
Treatment - Drugs

2

Description
Control group: Probiotic and vitamin D placebos capsule (Barij essence, Kashan, Iran), probiotic placebo daily and vitamin D placebo every 2 weeks, for 6 weeks orally.
Category
Treatment - Drugs

3

Description
Intervention group: Combined probiotic, including 2×109 Lactobacillus acidophilus, 2×109 Bifidobacterium bifidum, 2×109 Lactobacillus reuteri, 2×109 Lactobacillus fermentum daily, and vitamin D supplements (Zahravi, Tabriz, Iran), 50,000 IU vitamin D every 2 weeks, for 6 weeks orally.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Kosar gynecology Clinic
Full name of responsible person
Mehri Jamilian
Street address
Emam Khomeyni Avenue, Arak
City
Arak
Province
Markazi
Postal code
3814113634
Phone
+98 86 3223 3823
Email
jamilian.mehri@gmail.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Vice chancellor for research, Arak University of Medical Sciences
Full name of responsible person
Mohammad Rafiee
Street address
Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
City
Arak
Province
Markazi
Postal code
3814113634
Phone
+98 86 3223 3823
Email
rafiee-m@aums.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Vice chancellor for research, Arak University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
1771844351
Phone
+98 31 5546 3378
Fax
Email
asemi_r@yahoo.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
1771844351
Phone
+98 31 5546 3378
Fax
Email
asemi_r@yahoo.com
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
1771844351
Phone
+98 31 5546 3378
Fax
Email
asemi_r@yahoo.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
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