History
# Registration date Revision Id
3 2023-12-31, 1402/10/10 290455
2 2023-12-20, 1402/09/29 289348
1 2023-03-26, 1402/01/06 259830
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  • Protocol summary

    Migraine patients will be selected from the patients referred to Khorshid Clinic in Isfahan city. Then the patients will be divided into two intervention (black grape seed extract) and control (placebo) groups. The duration of the intervention will be eight weeks. Anthropometric measures and biochemical factors will be assessed at the baseline and end of the intervention.
    Migraine patients will be selected from the patients referred to the neurology clinic of Khorshid Hospital in Isfahan City. Then the patients will be divided into two intervention (black grape seed extract) and control (placebo) groups. The duration of the intervention will be eight weeks. Anthropometric measures and biochemical factors will be assessed at the baseline and end of the intervention.
    بیماران مبتلا به میگرن از بین بیماران مراجعه کننده به کلینیک خورشید شهر اصفهان انتخاب خواهند شد.سپس بیماران وارد دو گروه مداخله (عصاره هسته انگور سیاه) و کنترل (دارونما) می شوند. مدت مداخله هشت هفته خواهد بود. اندازه گیری های آنتروپومتریک و ارزیابی های بیوشیمیایی در ابتدا و انتهای مطالعه انجام خواهد شد.
    بیماران مبتلا به میگرن از بین بیماران مراجعه کننده به کلینیک مغز و اعصاب بیمارستان خورشید شهر اصفهان انتخاب خواهند شد.سپس بیماران وارد دو گروه مداخله (عصاره هسته انگور سیاه) و کنترل (دارونما) می شوند. مدت مداخله هشت هفته خواهد بود. اندازه گیری های آنتروپومتریک و ارزیابی های بیوشیمیایی در ابتدا و انتهای مطالعه انجام خواهد شد.
    Inclusion criteria: patients who are willing to participate in the study, diagnosed with migraine by a neurologist and according to the criteria of the International Classification of Headache Disorders-3 (ICHD-3), age 20 to 60 years, having at least one migraine attack per month, body mass index between 18.5 to 30. Exclusion criteria: suffering from cardiovascular, liver, kidney, and thyroid diseases, hypertension, diabetes, malignancies, other neurological disorders, and tension headaches, change in the type of medicine used and its dosage during the last month, pregnancy, and lactation, taking supplements containing antioxidants and black grape seed extract in the past 3 months, following a special diet, smoking
    Inclusion criteria: patients who are willing to participate in the study, diagnosed with migraine by a neurologist and according to the criteria of the International Classification of Headache Disorders-3 (ICHD-3), age 18 to 60 years, having at least one migraine attack per month, body mass index between 18.5 to 30. Non-inclusion criteria: suffering from tension-type headaches, suffering from chronic kidney diseases, cancer, and other neurological disorders, change in the type of medicine used and its dosage during the last month, pregnancy, and lactation, taking supplements containing antioxidants and black grape seed extract in the past 3 months, following a special diet
    شرایط ورود: بیمارانی که تمایل به شرکت در مطالعه داشته باشند، تشخیص ابتلا به میگرن توسط متخصص مغز و اعصاب و بر اساس معیار های طبقه بندی بین المللی اختلالات سردرد (ICHD-3:International Classification of Headache Disorders-3)، سن 20 تا 60 سال، داشتن حداقل یک حمله میگرنی در ماه، نمایه توده بدنی بین 18.5 تا 30. معیار های عدم ورود: ابتلا به بیماری های قلبی و عروقی،کبدی، کلیوی و تیروئید، فشار خون بالا، دیابت بدخیمی ها و سایر اختلالات عصبی و سردرد های تنشی، تغییر نوع داروی مصرفی و دوز آن طی یک ماه گذشته، بارداری و شیردهی، مصرف مکمل های حاوی آنتی اکسیدان و عصاره هسته انگور سیاه در 3 ماه گذشته، پیروی از رژیم غذایی خاص، استعمال دخانیات
    شرایط ورود: بیمارانی که تمایل به شرکت در مطالعه داشته باشند، تشخیص ابتلا به میگرن توسط متخصص مغز و اعصاب و بر اساس معیار های طبقه بندی بین المللی اختلالات سردرد (ICHD-3:International Classification of Headache Disorders-3)، سن 18 تا 60 سال، داشتن حداقل یک حمله میگرنی در ماه، نمایه توده بدنی بین 18.5 تا 30. معیار های عدم ورود: ابتلا به سردرد های تنشی، ابتلا به بیماری های مزمن کلیوی، سرطان و سایر اختلالات عصبی، تغییر نوع داروی مصرفی و دوز آن طی یک ماه گذشته، بارداری و شیردهی، مصرف مکمل های حاوی آنتی اکسیدان و عصاره هسته انگور سیاه در 3 ماه گذشته، پیروی از رژیم غذایی خاص
    Intervention group: Daily two capsules containing black grape seed extract (Daru Pajhuh-e-Jaber company) before lunch and dinner for eight weeks. Control group: Two placebo capsules per day (Daru Pajhuh-e-Jaber company) for eight weeks.
    Intervention group: Daily two capsules containing black grape seed extract (Daru Pajhuh-e-Jaber company) for eight weeks. Control group: Two placebo capsules per day (Daru Pajhuh-e-Jaber company) for eight weeks.
    گروه مداخله: روزانه دو عدد کپسول حاوی عصاره هسته انگور سیاه (شرکت دارو پژوه جابر) قبل از وعده نهار و شام به مدت هشت هفته. گروه کنترل: روزانه دو عدد کپسول دارونما (شرکت دارو پژوه جابر) به مدت هشت هفته
    گروه مداخله: روزانه دو عدد کپسول حاوی عصاره هسته انگور سیاه (شرکت دارو پژوه جابر) به مدت هشت هفته. گروه کنترل: روزانه دو عدد کپسول دارونما (شرکت دارو پژوه جابر) به مدت هشت هفته
  • General information

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    An update was made in the inclusion and non-inclusion criteria. Because they were very restrictive
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    به روز رسانی در معیار های ورود به مطالعه و عدم ورود به مطالعه انجام شد. زیرا بسیار محدود کننده بودند.
    Patients who are willing to participate in the study
    Migraine diagnosis by a neurologist based on the International Classification of Headache Disorders-3 (ICHD-3)
    Age 20 to 60 years
    Having at least one migraine attack per month
    Body mass index between 18.5 to 30
    Patients who are willing to participate in the study
    Migraine diagnosis by a neurologist based on the International Classification of Headache Disorders-3 (ICHD-3)
    Age 18 to 60 years
    Having at least one migraine attack per month
    Body mass index between 18.5 to 30
    بیمارانی که تمایل به شرکت در مطالعه داشته باشند
    تشخیص ابتلا به میگرن توسط متخصص مغز و اعصاب و بر اساس معیار های طبقه بندی بین المللی اختلالات سردرد (ICHD-3:International Classification of Headache Disorders-3)
    سن 20 تا 60 سال
    داشتن حداقل یک حمله میگرنی در ماه
    نمایه توده بدنی بین 18.5 تا 30
    بیمارانی که تمایل به شرکت در مطالعه داشته باشند
    تشخیص ابتلا به میگرن توسط متخصص مغز و اعصاب و بر اساس معیار های طبقه بندی بین المللی اختلالات سردرد (ICHD-3:International Classification of Headache Disorders-3)
    سن 18 تا 60 سال
    داشتن حداقل یک حمله میگرنی در ماه
    نمایه توده بدنی بین 18.5 تا 30
    Suffering from cardiovascular, liver, kidney, and thyroid diseases, high blood pressure, diabetes, malignancies, and other neurological disorders
    Suffering from tension headaches
    Change in the type of medicine used and its dosage during the last month
    Pregnancy and lactation
    Taking supplements containing antioxidants and black grape seed extract in the last 3 months
    Following a special diet (vegetarian, Atkins, and ketogenic diets)
    ُSmoking
    Suffering from chronic kidney diseases, cancer, and other neurological disorders
    Suffering from tension-type headaches
    Change in the type of medicine used and its dosage during the last month
    Pregnancy and lactation
    Taking supplements containing antioxidants and black grape seed extract in the last 3 months
    Following a special diet
    ابتلا به بیماری های قلبی و عروقی، کبدی، کلیوی و تیروئید، فشار خون بالا، دیابت، بدخیمی ها و سایر اختلالات عصبی
    ابتلا به سردرد های تنشی
    تغییر نوع داروی مصرفی و دوز آن طی یک ماه گذشته
    بارداری و شیردهی
    مصرف مکمل های حاوی آنتی اکسیدان و عصاره هسته انگور سیاه در 3 ماه گذشته
    پیروی از رژیم غذایی خاص (رژیم های گیاهخواری، اتکینز و کتوژنیک)
    استعمال دخانیات
    ابتلا به بیماری های مزمن کلیوی، سرطان و سایر اختلالات عصبی
    ابتلا به سردرد های تنشی
    تغییر نوع داروی مصرفی و دوز آن طی یک ماه گذشته
    بارداری و شیردهی
    مصرف مکمل های حاوی آنتی اکسیدان و عصاره هسته انگور سیاه در 3 ماه گذشته
    پیروی از رژیم غذایی خاص
    This study is a double-blind clinical trial study (participant, outcome assessor). The black grape seed extract supplement and its placebo (identical in terms of color, shape, and smell) will be packaged in similar boxes, and the participants and the outcome assessor will not be informed of the contents of the packages until the end of the study.
    This study is a double-blind clinical trial study (participant, outcome assessor). The black grape seed extract supplement and its placebo (identical in terms of color, shape, and taste) will be packaged in similar boxes, and the participants and the outcome assessor will not be informed of the contents of the packages until the end of the study.
    این مطالعه یک مطالعه کارآزمایی بالینی دو سوکور (شرکت کننده، ارزیابی کننده پیامد) می باشد. مکمل عصاره هسته انگور سیاه و دارونمای آن (به طور یکسان از لحاظ رنگ، شکل و بو) در جعبه های مشابه بسته بندی خواهند شد و شرکت کنندگان و ارزیابی کننده پیامد تا انتهای مطالعه از محتوای بسته ها مطلع نخواهند شد.
    این مطالعه یک مطالعه کارآزمایی بالینی دو سوکور (شرکت کننده، ارزیابی کننده پیامد) می باشد. مکمل عصاره هسته انگور سیاه و دارونمای آن (به طور یکسان از لحاظ رنگ، شکل و مزه) در جعبه های مشابه بسته بندی خواهند شد و شرکت کنندگان و ارزیابی کننده پیامد تا انتهای مطالعه از محتوای بسته ها مطلع نخواهند شد.
  • Primary outcomes

    #1
    Quality of life in migraine patients
    Migraine-induced disability
    کیفیت زندگی بیماران میگرن
    ناتوانی ناشی از میگرن
    Migraine-Specific Quality of Life (MSQ)
    Headache Impact Test-6 questionnaire (HIT-6)
    پرسشنامه اختصاصی کیفیت زندگی بیماران میگرن
    پرسشنامه تست تاثیر سردرد-6
    #2
    Migraine-induced disability
    Calcitonin gene-related peptide (CGRP) serum level
    ناتوانی ناشی از میگرن
    سطح سرمی پپتید مرتبط با ژن کلسی تونین
    Headache Impact Test-6 questionnaire (HIT-6)
    Blood test (ELISA)
    پرسشنامه تست تاثیر سردرد-6
    آزمایش خون (الایزا)
    #3
    Depression
    Vascular cell adhesion molecule-1 (VCAM-1) serum level
    افسردگی
    سطح سرمی مولکول چسبنده سلول عروق-1
    Depression Anxiety and Stress Scale-21 questionnaire (DASS-21)
    Blood test (ELISA)
    پرسشنامه افسردگی، اضطراب و استرس -21
    آزمایش خون (الایزا)
    #4
    Calcitonin gene-related peptide (CGRP) serum level
    Malondialdehyde (MDA) serum level
    سطح سرمی پپتید مرتبط با ژن کلسی تونین
    سطح سرمی مالون دی آلدهید
    Blood test (ELISA)
    Blood test (colorimetric method)
    آزمایش خون (الایزا)
    آزمایش خون (روش رنگ سنجی)
    #5
    Vascular cell adhesion molecule-1 (VCAM-1) serum level
    Serum level of total antioxidant capacity (TAC)
    سطح سرمی مولکول چسبنده سلول عروق-1
    سطح سرمی ظرفیت تام آنتی اکسیدانی
    Blood test (ELISA)
    Blood test (colorimetric method)
    آزمایش خون (الایزا)
    آزمایش خون (روش رنگ سنجی)
    #6
    Malondialdehyde (MDA) serum level
    Migraine frequency
    سطح سرمی مالون دی آلدهید
    فرکانس میگرن
    Blood test (colorimetric method)
    Question / Number of attacks per month
    آزمایش خون (روش رنگ سنجی)
    پرسش/ تعداد حملات در ماه
    #7
    Serum level of total antioxidant capacity (TAC)
    Duration of migraine headache
    سطح سرمی ظرفیت تام آنتی اکسیدانی
    طول مدت سردرد میگرنی
    Blood test (colorimetric method)
    Question/ average duration of migraine attacks per headache (hours)
    آزمایش خون (روش رنگ سنجی)
    پرسش/ میانگین مدت حملات میگرن در هر بار سردرد (ساعت)
    #8
    Anxiety
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    اضطراب
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    Depression Anxiety and Stress Scale-21 questionnaire (DASS-21)
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    پرسشنامه افسردگی، اضطراب و استرس -21
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    #9
    Stress
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    استرس
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    Depression Anxiety and Stress Scale-21 questionnaire (DASS-21)
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    پرسشنامه افسردگی، اضطراب و استرس -21
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    #10
    Migraine frequency
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    فرکانس میگرن
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    Question / Number of attacks per month
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    پرسش/ تعداد حملات در ماه
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    #11
    Duration of migraine headache
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    طول مدت سردرد میگرنی
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    Question/ average duration of migraine attacks per headache (hours)
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    پرسش/ میانگین مدت حملات میگرن در هر بار سردرد (ساعت)
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  • Secondary outcomes

    #1
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    Quality of life in migraine patients
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    کیفیت زندگی بیماران میگرن
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    Migraine-Specific Quality of Life (MSQ)
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    پرسشنامه اختصاصی کیفیت زندگی بیماران میگرن
    #2
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    Depression
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    افسردگی
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    Depression Anxiety and Stress Scale-21 questionnaire (DASS-21)
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    پرسشنامه افسردگی، اضطراب و استرس -21
    #3
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    Anxiety
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    اضطراب
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    Depression Anxiety and Stress Scale-21 questionnaire (DASS-21)
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    پرسشنامه افسردگی، اضطراب و استرس -21
    #4
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    Stress
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    استرس
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    Depression Anxiety and Stress Scale-21 questionnaire (DASS-21)
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    پرسشنامه افسردگی، اضطراب و استرس -21
    #5
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    Blood pressure
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    فشارخون
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    Mercury barometer
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    فشارسنج جیوه ای
    #6
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    Anthropometric indices
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    شاخص های آنتروپومتریک
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    Before the start of the intervention and 60 days after the start of the intervention
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    قبل از شروع مداخله و 60 روز پس از شروع مداخله
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    A digital scale and standard stadiometer
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    ترازو دیجیتال و قدسنج استاندارد
  • Intervention groups

    #1
    Intervention group: Two capsules of black grape seed extract daily (containing 100 mg of black grape seed extract, Daru Pajhuh-e-Jaber company) before lunch and dinner for eight weeks
    Intervention group: Two capsules of black grape seed extract daily (containing 100 mg of black grape seed extract, Daru Pajhuh-e-Jaber company) for eight weeks
    گروه مداخله: روزانه دو عدد کپسول عصاره هسته انگور سیاه (حاوی 100 میلی گرم عصاره هسته انگور سیاه، شرکت دارو پژوه جابر) قبل از ناهار و شام به مدت هشت هفته
    گروه مداخله: روزانه دو عدد کپسول عصاره هسته انگور سیاه (حاوی 100 میلی گرم عصاره هسته انگور سیاه، شرکت دارو پژوه جابر) به مدت هشت هفته
    #2
    Control group: Two placebo capsules daily (containing calcium phosphate, Avicel, and gelatin, Daru Pajhuh-e-Jaber company) before lunch and dinner for eight weeks.
    Control group: Two placebo capsules daily (containing calcium phosphate, Avicel, and gelatin, Daru Pajhuh-e-Jaber company) for eight weeks.
    گروه کنترل: روزانه دو عدد کپسول دارونما ( حاوی کلسیم فسفات، آویسل و ژلاتین، شرکت دارو پژوه جابر) قبل از ناهار و شام به مدت هشت هفته
    گروه کنترل: روزانه دو عدد کپسول دارونما ( حاوی کلسیم فسفات، آویسل و ژلاتین، شرکت دارو پژوه جابر) به مدت هشت هفته
  • Recruitment centers

    #1
    Name of recruitment center - English: Khorshid clinic
    Name of recruitment center - Persian: کلنیک خورشید
    Full name of responsible person - English: Dr. Gholamreza Askari
    Full name of responsible person - Persian: دکتر غلامرضا عسکری
    Street address - English: Ostandari St.
    Street address - Persian: خیابان استانداری
    City - English: Isfahan
    City - Persian: اصفهان
    Province: Isfehan
    Country: Iran (Islamic Republic of)
    Postal code: 8174673461
    Phone: +98 31 3792 3171
    Fax:
    Email: Askari@mui.ac.ir
    Web page address:
    Name of recruitment center - English: Neurology clinic of Khorshid hospital
    Name of recruitment center - Persian: کلینیک مغز و اعصاب بیمارستان خورشید
    Full name of responsible person - English: Dr. Gholamreza Askari
    Full name of responsible person - Persian: دکتر غلامرضا عسکری
    Street address - English: khorshid medical educational research complex, Ostandari Street, Isfahan
    Street address - Persian: اصفهان- خیابان استانداری- مجتمع آموزشی درمانی وپژوهشی خورشید
    City - English: Isfahan
    City - Persian: اصفهان
    Province: Isfehan
    Country: Iran (Islamic Republic of)
    Postal code: 8145833117
    Phone: +98 31 3222 2127
    Fax:
    Email: Askari@mui.ac.ir
    Web page address:
  • Sponsors / Funding sources

    #1

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Dr. Gholamreza Askari
    Full name of responsible person - Persian: دکتر غلامرضا عسکری
    Street address - English: Deputy of Research & Technology of Isfahan University of Medical Science, Hezar Jarib Street
    Street address - Persian: اصفهان، خیابان هزار جریب، معاونت پژوهشی و فناوری دانشگاه علوم پزشکی اصفهان
    City - English: Isfahan
    City - Persian: اصفهان
    Province: Isfehan
    Country: Iran (Islamic Republic of)
    Postal code: 8174673461
    Phone: +98 31 3792 3060
    Fax:
    Email: askari@mui.ac.ir
    Web page address: https://research.mui.ac.ir

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Dr. Gholamreza Askari
    Full name of responsible person - Persian: دکتر غلامرضا عسکری
    Street address - English: Deputy of Research & Technology of Isfahan University of Medical Sciences, Hezar Jarib Street, Isfahan
    Street address - Persian: اصفهان، خیابان هزار جریب، معاونت پژوهشی و فناوری دانشگاه علوم پزشکی اصفهان
    City - English: Isfahan
    City - Persian: اصفهان
    Province: Isfehan
    Country: Iran (Islamic Republic of)
    Postal code: 8174673461
    Phone: +98 31 3792 3060
    Fax:
    Email: askari@mui.ac.ir
    Web page address: https://research.mui.ac.ir

Protocol summary

Study aim
The effect of black grape seed extract supplementation on inflammatory biomarkers, oxidative stress, clinical symptoms, and quality of life in patients with migraine
Design
A randomized controlled clinical trial with parallel groups, double-blind, on 50 patients. Stratified randomization based on migraine type and body mass index
Settings and conduct
Migraine patients will be selected from the patients referred to the neurology clinic of Khorshid Hospital in Isfahan City. Then the patients will be divided into two intervention (black grape seed extract) and control (placebo) groups. The duration of the intervention will be eight weeks. Anthropometric measures and biochemical factors will be assessed at the baseline and end of the intervention.
Participants/Inclusion and exclusion criteria
Inclusion criteria: patients who are willing to participate in the study, diagnosed with migraine by a neurologist and according to the criteria of the International Classification of Headache Disorders-3 (ICHD-3), age 18 to 60 years, having at least one migraine attack per month, body mass index between 18.5 to 30. Non-inclusion criteria: suffering from tension-type headaches, suffering from chronic kidney diseases, cancer, and other neurological disorders, change in the type of medicine used and its dosage during the last month, pregnancy, and lactation, taking supplements containing antioxidants and black grape seed extract in the past 3 months, following a special diet
Intervention groups
Intervention group: Daily two capsules containing black grape seed extract (Daru Pajhuh-e-Jaber company) for eight weeks. Control group: Two placebo capsules per day (Daru Pajhuh-e-Jaber company) for eight weeks.
Main outcome variables
Migraine symptoms, patients' quality of life, calcitonin gene-related peptide, vascular cell adhesion molecule-1, oxidative stress status

General information

Reason for update
An update was made in the inclusion and non-inclusion criteria. Because they were very restrictive
Acronym
IRCT registration information
IRCT registration number: IRCT20121216011763N56
Registration date: 2023-03-26, 1402/01/06
Registration timing: prospective

Last update: 2023-12-23, 1402/10/02
Update count: 2
Registration date
2023-03-26, 1402/01/06
Registrant information
Name
Gholamreza Askari
Name of organization / entity
Isfahan University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 31 1792 2110
Email address
askari@mui.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2023-06-22, 1402/04/01
Expected recruitment end date
2023-09-23, 1402/07/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of black grape seed extract supplementation on inflammatory biomarkers, oxidative stress, clinical symptoms, and quality of life in patients with migraine
Public title
The effect of black grape seed extract supplement in patients with migraine
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Patients who are willing to participate in the study Migraine diagnosis by a neurologist based on the International Classification of Headache Disorders-3 (ICHD-3) Age 18 to 60 years Having at least one migraine attack per month Body mass index between 18.5 to 30
Exclusion criteria:
Suffering from chronic kidney diseases, cancer, and other neurological disorders Suffering from tension-type headaches Change in the type of medicine used and its dosage during the last month Pregnancy and lactation Taking supplements containing antioxidants and black grape seed extract in the last 3 months Following a special diet
Age
From 18 years old to 60 years old
Gender
Both
Phase
3
Groups that have been masked
  • Participant
  • Outcome assessor
Sample size
Target sample size: 50
Randomization (investigator's opinion)
Randomized
Randomization description
Randomization was done based on the stratified randomization method. To distribute patients into intervention and control groups, first, persons are classified into four states according to the type of migraine (with aura/without aura) and body mass index (18.5 to 24.9 and 25 to 30). Then, persons with the same conditions will be randomly assigned to two intervention and control groups. For this purpose, the first person who referred will be in the intervention group and the other person with the same conditions will be in the control group.
Blinding (investigator's opinion)
Double blinded
Blinding description
This study is a double-blind clinical trial study (participant, outcome assessor). The black grape seed extract supplement and its placebo (identical in terms of color, shape, and taste) will be packaged in similar boxes, and the participants and the outcome assessor will not be informed of the contents of the packages until the end of the study.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics Committee of Isfahan University of Medical Sciences
Street address
Isfahan University of Medical Sciences, Hezar Jerib street, Isfahan, Iran
City
Isfahan
Province
Isfehan
Postal code
8174673461
Approval date
2023-03-07, 1401/12/16
Ethics committee reference number
IR.MUI.RESEARCH.REC.1401.405

Health conditions studied

1

Description of health condition studied
Migraine
ICD-10 code
G43
ICD-10 code description
Migraine

Primary outcomes

1

Description
Severity of migraine attacks
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Visual Analogue Scale (VAS)

2

Description
Migraine-induced disability
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Headache Impact Test-6 questionnaire (HIT-6)

3

Description
Calcitonin gene-related peptide (CGRP) serum level
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Blood test (ELISA)

4

Description
Vascular cell adhesion molecule-1 (VCAM-1) serum level
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Blood test (ELISA)

5

Description
Malondialdehyde (MDA) serum level
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Blood test (colorimetric method)

6

Description
Serum level of total antioxidant capacity (TAC)
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Blood test (colorimetric method)

7

Description
Migraine frequency
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Question / Number of attacks per month

8

Description
Duration of migraine headache
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Question/ average duration of migraine attacks per headache (hours)

Secondary outcomes

1

Description
Quality of life in migraine patients
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Migraine-Specific Quality of Life (MSQ)

2

Description
Depression
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Depression Anxiety and Stress Scale-21 questionnaire (DASS-21)

3

Description
Anxiety
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Depression Anxiety and Stress Scale-21 questionnaire (DASS-21)

4

Description
Stress
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Depression Anxiety and Stress Scale-21 questionnaire (DASS-21)

5

Description
Blood pressure
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
Mercury barometer

6

Description
Anthropometric indices
Timepoint
Before the start of the intervention and 60 days after the start of the intervention
Method of measurement
A digital scale and standard stadiometer

Intervention groups

1

Description
Intervention group: Two capsules of black grape seed extract daily (containing 100 mg of black grape seed extract, Daru Pajhuh-e-Jaber company) for eight weeks
Category
Treatment - Drugs

2

Description
Control group: Two placebo capsules daily (containing calcium phosphate, Avicel, and gelatin, Daru Pajhuh-e-Jaber company) for eight weeks.
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Neurology clinic of Khorshid hospital
Full name of responsible person
Dr. Gholamreza Askari
Street address
khorshid medical educational research complex, Ostandari Street, Isfahan
City
Isfahan
Province
Isfehan
Postal code
8145833117
Phone
+98 31 3222 2127
Email
Askari@mui.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Esfahan University of Medical Sciences
Full name of responsible person
Dr. Gholamreza Askari
Street address
Deputy of Research & Technology of Isfahan University of Medical Sciences, Hezar Jarib Street, Isfahan
City
Isfahan
Province
Isfehan
Postal code
8174673461
Phone
+98 31 3792 3060
Email
askari@mui.ac.ir
Web page address
https://research.mui.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Esfahan University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Esfahan University of Medical Sciences
Full name of responsible person
Dr. Gholamreza Askari
Position
Professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Deputy of Research & Technology of Isfahan University of Medical Sciences, Hezar Jarib Street, Isfahan
City
Isfahan
Province
Isfehan
Postal code
8174673461
Phone
+98 31 3792 3060
Email
askari@mui.ac.ir
Web page address
https://research.mui.ac.ir

Person responsible for scientific inquiries

Contact
Name of organization / entity
Esfahan University of Medical Sciences
Full name of responsible person
Dr. Gholamreza Askari
Position
Professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Deputy of Research & Technology of Isfahan University of Medical Sciences, Hezar Jarib Street, Isfahan
City
Isfahan
Province
Isfehan
Postal code
8174673461
Phone
+98 31 3792 3060
Email
askari@mui.ac.ir
Web page address
https://research.mui.ac.ir

Person responsible for updating data

Contact
Name of organization / entity
Esfahan University of Medical Sciences
Full name of responsible person
Dr. Gholamreza Askari
Position
Professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Deputy of Research & Technology of Isfahan University of Medical Sciences, Hezar Jarib Street, Isfahan
City
Isfahan
Province
Isfehan
Postal code
8174673461
Phone
+98 31 3792 3060
Email
askari@mui.ac.ir

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
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