History
# Registration date Revision Id
2 2024-11-02, 1403/08/12 320027
1 2024-02-03, 1402/11/14 293447
Changes made to previous revision
  • Help:

    Red color represents old content. It may be empty showing addition in the new version.
    Green color represents new content. It may be empty showing deletion in the new version.

    Inline Side by side
    Added new contents, deleted old contents, contents that are not changed.
    New table contents New table contents
    Old table contents Old table contents
    Unchanged contents Unchanged contents
    Added new contents, contents that are not changed.
    Deleted old contents, contents that are not changed.
    Old table contents Old table contents
    Unchanged contents Unchanged contents
    New table contents New table contents
    Unchanged contents Unchanged contents
  • Protocol summary

    60 patients with non-alcoholic fatty liver disease will be randomly assigned to intervention and control groups. In the intervention group, participants will receive a daily capsule of berberis integrimma extract (containing 320 mg of total anthocyanin). In the placebo group, people take a placebo capsule. The study period will be 12 weeks. A dose of 320 mg per day has been associated with improvement in inflammatory markers in previous studies. The DASH diet will be prescribed for both groups according to the characteristics of the participants. Also, to control participants in terms of taking supplements and placebo and prevent the samples from falling, the follow-up of patients would occur every two weeks by phone.
    60 patients with non-alcoholic fatty liver disease will be randomly assigned to intervention and control groups. In the intervention group, participants will receive 2 tablespoons of berberis integerimna extract daily. In the placebo group, participants consume the same amount of placebo. The study period will be 12 weeks. The DASH diet will be prescribed for both groups according to the characteristics of the participants. Also, to control participants in terms of taking supplements and placebo and prevent the samples from falling, the follow-up of patients would occur every two weeks by phone.
    60 بیمار مبتلا به کبد چرب غیر الکلی به دو گروه مداخله و کنترل به صورت تصادفی تخصیص خواهند شد. درگروه مداخله با عصاره میوه زرشک استاندارد شده بر مبنای آنتوسیانین تام، افراد روزانه یک کپسول عصاره زرشک(حاوی 320 میلی گرم آنتوسیانین تام) دریافت می کنند. در گروه دارونما نیز افراد یک کپسول دارونما مصرف می کنند. مدت مطالعه نیز 12 هفته خواهد بود. دوز 320 میلی گرم در روز با بهبود شاخص های التهابی در مطالعات گذشته در ارتباط بوده است. رژیم غذای DASH برای هر دو گروه با توجه به مشخصات و نبازهای فردی افراد تجویز خواهد شد.همچنین به منظور کنترل شرکت کنندگان از نظر مصرف مکمل ها، دارونما و همچنین جلوگیری از ریزش نمونه ها، پیگیری بیماران هر دو هفته یکبار به صورت تلفنی انجام می شود.
    60 بیمار مبتلا به کبد چرب غیر الکلی به دو گروه مداخله و کنترل به صورت تصادفی تخصیص خواهند شد. درگروه مداخله افراد روزانه 2 قاشق غذاخوری عصاره زرشک کوهی دریافت می کنند. در گروه دارونما نیز افراد همین میزان دارونما مصرف می کنند. مدت مطالعه نیز 12 هفته خواهد بود. رژیم غذای DASH برای هر دو گروه با توجه به مشخصات و نبازهای فردی افراد تجویز خواهد شد.همچنین به منظور کنترل شرکت کنندگان از نظر مصرف مکمل ها، دارونما و همچنین جلوگیری از ریزش نمونه ها، پیگیری بیماران هر دو هفته یکبار به صورت تلفنی انجام می شود.
    In the intervention group, people receive a daily capsule of barberry extract (containing 320 mg of total anthocyanin). In the placebo group, people take a placebo capsule. The study period will be 12 weeks. A dose of 320 mg per day has been associated with improvement in inflammatory markers in previous studies.
    In the intervention group, participants receive 2 tablespoons of berberis integerimna extract daily. In the placebo group, participants consume the same amount of placebo. The study period will be 12 weeks.
    درگروه مداخله با عصاره میوه زرشک استاندارد شده بر مبنای آنتوسیانین تام، افراد روزانه یک کپسول عصاره زرشک(حاوی 320 میلی گرم آنتوسیانین تام) دریافت می کنند. در گروه دارونما نیز افراد یک کپسول دارونما مصرف می کنند. مدت مطالعه نیز 12 هفته خواهد بود. دوز 320 میلی گرم در روز با بهبود شاخص های التهابی در مطالعات گذشته در ارتباط بوده است
    درگروه مداخله افراد روزانه 2 قاشق غذاخوری عصاره زرشک کوهی دریافت می کنند. در گروه دارونما نیز افراد همین میزان دارونما مصرف می کنند. مدت مطالعه نیز 12 هفته خواهد بود.
  • General information

    empty
    1. Cooperation and sponsorship of Sanabel Daru Company to prepare the extract 2. Using berberis integerrima syrup instead of berberis integerrima capsules
    empty
    1. همکاری با شرکت سنابل دارو و استفاده از حمایت مالی این شرکت جهت تهیه عصاره 2. استفاده از شربت زرشک کوهی به جای کپسول زرشک کوهی
  • Intervention groups

    #1
    Intervention group: In the intervention group, participants will receive a daily capsule of barberry extract (containing 320 mg of total anthocyanin) for 12 weeks. The DASH diet will be prescribed for both groups according to their characteristics and individual requirements.
    Intervention group: In the intervention group, participants will receive 2 tablespoons of barberry extract (Prepared by Sanabel Daru Company) for 12 weeks. The DASH diet will be prescribed for both groups according to their characteristics and individual requirements.
    گروه مداخله: درگروه مداخله با عصاره میوه زرشک استاندارد شده بر مبنای آنتوسیانین تام، افراد روزانه یک کپسول عصاره زرشک(حاوی 320 میلی گرم آنتوسیانین تام) را به مدت 12 هفته دریافت می کنند. رژیم غذای DASH برای هر دو گروه با توجه به مشخصات و نبازهای فردی افراد تجویز خواهد شد.
    گروه مداخله: درگروه مداخله، افراد روزانه 2 قاشق غذاخوری عصاره زرشک کوهی (تهیه شده توسط شرکت سنابل دارو) را به مدت 12 هفته دریافت می کنند. رژیم غذای DASH برای هر دو گروه با توجه به مشخصات و نبازهای فردی افراد تجویز خواهد شد.
    #2
    Control group: In the placebo group, participants will receive a placebo capsule with a similar appearance to the intervention capsules, for 12 weeks.
    Control group: In the placebo group, participants will receive a placebo syrup with a similar appearance to the intervention syrup, for 12 weeks.
    گروه کنترل: در گروه دارونما افراد یک کپسول دارونما که از لحاظ ظاهری کاملا با کپسول های گروه مداخله مشابه خواهد بود، به مدت 12 هفته مصرف خواهند کرد.
    گروه کنترل: در گروه دارونما افراد 2 قاشق غذاخوری دارونما که از لحاظ ظاهری کاملا با شربت های گروه مداخله مشابه خواهد بود، به مدت 12 هفته مصرف خواهند کرد.
  • Recruitment centers

    #1
    Name of recruitment center - English: Dr. Mohammad Hossein Ahrarizdi's clinic
    Name of recruitment center - Persian: مطب دکتر محمدحسین احراریزدی
    Full name of responsible person - English: sayyed saeid khayyatzadeh
    Full name of responsible person - Persian: سید سعید خیاط زاده
    Street address - English: Maryam Medical building, Maryam Alley, Kashani St., Yazd
    Street address - Persian: یزد - یزد خیابان کاشانی نرسیده به سه راه چمران (هراتی) کوچه مریم ساختمان پزشکان مریم
    City - English: Yazd
    City - Persian: یزد
    Province: Yazd
    Country: Iran (Islamic Republic of)
    Postal code: ۸۹۱۵۱۷۳۱۶۰
    Phone: +98 35 3623 0765
    Fax:
    Email: khayyatzadeh@yahoo.com
    Web page address:
    Name of recruitment center - English: Dr. Mohammad Hossein Ahrarizdi's clinic
    Name of recruitment center - Persian: مطب دکتر محمد حسین احرار یزدی
    Full name of responsible person - English: sayyed saeid khayyatzadeh
    Full name of responsible person - Persian: سید سعید خیاط زاده
    Street address - English: Maryam Medical building, Maryam Alley, Kashani St., Yazd
    Street address - Persian: یزد - یزد خیابان کاشانی نرسیده به سه راه چمران (هراتی) کوچه مریم ساختمان پزشکان مریم
    City - English: Yazd
    City - Persian: یزد
    Province: Yazd
    Country: Iran (Islamic Republic of)
    Postal code: 8915173160
    Phone: +98 35 3623 0765
    Fax:
    Email: khayyatzadeh@yahoo.com
    Web page address:
  • Sponsors / Funding sources

    #1

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Amin Salehi abargouei
    Full name of responsible person - Persian: امین صالحی ابرقوئی
    Street address - English: Central Administration, Bahonar Sq., Yazd 8916978477 Iran
    Street address - Persian: یزد – میدان امام حسین (ع) – ابتدای بلوار دانشجو – مجتمع آموزشی پژوهشی امام رضا(ع) – معاونت تحقیقات و فناوری دانشگاه علوم پزشکی شهید صدوقی یزد
    City - English: Yazd
    City - Persian: یزد
    Province: Yazd
    Country: Iran (Islamic Republic of)
    Postal code: ۸۹۱۶۱۸۸۶۳۷
    Phone: +98 35 3628 8114
    Fax:
    Email: dvc.research@ssu.ac.ir
    Web page address:

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Amin Salehi abargouei
    Full name of responsible person - Persian: امین صالحی ابرقوئی
    Street address - English: Central Administration, Bahonar Sq., Yazd
    Street address - Persian: یزد – میدان امام حسین (ع) – ابتدای بلوار دانشجو – مجتمع آموزشی پژوهشی امام رضا(ع) – معاونت تحقیقات و فناوری دانشگاه علوم پزشکی شهید صدوقی یزد
    City - English: Yazd
    City - Persian: یزد
    Province: Yazd
    Country: Iran (Islamic Republic of)
    Postal code: 8916978477
    Phone: +98 35 3628 8114
    Fax:
    Email: dvc.research@ssu.ac.ir
    Web page address:
    100
    50
    #2
    contact.organization_id:
    Name of organization / entity - English: Sanabel Daroo Company
    Name of organization / entity - Persian: شرکت سنابل دارو
    Full name of responsible person - English: Dr Mohsen Naseri
    Full name of responsible person - Persian: دکتر محسن ناصری
    Street address - English: Unit 1, No. 78, West Nusrat St., Tohid Square, Tehran
    Street address - Persian: تهران، میدان توحید ، خیابان نصرت غربی، پلاک 78 واحد یک
    City - English: Tehran
    City - Persian: تهران
    Province: Tehran
    Country: Iran (Islamic Republic of)
    Postal code: 1457785693
    Phone: +98 996 058 6908
    Fax:
    Email: info@sanabel.ir
    Web page address: https://sanabel.ir/
    empty
    Yes
    empty
    50
    empty
    Private
    empty
    Domestic
    empty
    Industry
    empty
    Sanabel Daroo Company
    empty
    شرکت سنابل دارو

Protocol summary

Study aim
Investigating the effect of berberis integrimma extract on disease severity and inflammatory indices in patients with ulcerative colitis
Design
Parallel-groups, double-blind, randomized clinical controlled trial
Settings and conduct
60 patients with non-alcoholic fatty liver disease will be randomly assigned to intervention and control groups. In the intervention group, participants will receive 2 tablespoons of berberis integerimna extract daily. In the placebo group, participants consume the same amount of placebo. The study period will be 12 weeks. The DASH diet will be prescribed for both groups according to the characteristics of the participants. Also, to control participants in terms of taking supplements and placebo and prevent the samples from falling, the follow-up of patients would occur every two weeks by phone.
Participants/Inclusion and exclusion criteria
Entry criteria: age 18 years and older; diagnosis of the disease by ultrasound; ALT >30 IU/L in men and ALT >19 IU/L in women; BMI greater than 18.5 and less than 30. Non-entry criteria: pregnancy or breastfeeding; alcohol consumption; history of diseases including liver cirrhosis; viral hepatitis; cardiovascular diseases; cancer; relapse and hospitalization of the patient; allergy to barberry.
Intervention groups
In the intervention group, participants receive 2 tablespoons of berberis integerimna extract daily. In the placebo group, participants consume the same amount of placebo. The study period will be 12 weeks.
Main outcome variables
changes in the severity of liver steatosis; changes in the severity of liver fibrosis; Serum levels of liver enzymes (ALT, AST, ALP, GGT)

General information

Reason for update
1. Cooperation and sponsorship of Sanabel Daru Company to prepare the extract 2. Using berberis integerrima syrup instead of berberis integerrima capsules
Acronym
IRCT registration information
IRCT registration number: IRCT20210427051098N6
Registration date: 2024-02-03, 1402/11/14
Registration timing: prospective

Last update: 2024-11-10, 1403/08/20
Update count: 1
Registration date
2024-02-03, 1402/11/14
Registrant information
Name
Sayyed Saeid Khayyatzadeh
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 35 3820 9100
Email address
khayyatzadeh@yahoo.com
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2024-05-21, 1403/03/01
Expected recruitment end date
2025-05-22, 1404/03/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effects of berberis integerrima extract on liver enzymes, liver steatosis and fibrosis in patients with non-alcoholic fatty liver disease (NAFLD) following the DASH diet: a double-blind randomized clinical trial.
Public title
The effects of berberis integerrima extract on the non-alcoholic fatty liver disease (NAFLD)
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Age 18 and older Diagnosis of the disease by ultrasound with a standard method ALT >30 IU/L in men and ALT >19 IU/L in women BMI higher than 18.5 and less than 30
Exclusion criteria:
Taking corticosteroids Pregnancy and breastfeeding Alcohol consumption (men: ethanol > 140 g per week, women: ethanol > 70 g per week) History of diseases including liver cirrhosis, viral hepatitis, cardiovascular diseases, cancer Medication history including corticosteroids, non-steroidal anti-inflammatory drugs, drugs affecting blood sugar, lipid profile, body weight and liver function. Disease relapse and patient hospitalization Barberry allergy
Age
From 18 years old
Gender
Both
Phase
N/A
Groups that have been masked
  • Participant
  • Care provider
  • Investigator
  • Outcome assessor
  • Data analyser
Sample size
Target sample size: 60
Randomization (investigator's opinion)
Randomized
Randomization description
The random allocation between two groups will perform using Random allocation version 2 software. The random sequence will generate with this software using a simple random allocation method, and each number will allocate to an intervention group A or B. Allocation concealment will perform by calling or sending message to a third-person who is unaware of intervention.
Blinding (investigator's opinion)
Double blinded
Blinding description
Participants, investigators and data collectors will be blinded to the study groups. Allocation concealment will perform by calling or sending message to a third-person who is unaware of intervention.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Yazd Shahid Sadoughi University of Medical Sciences
Street address
School of public health, Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blvd., Alam Square, Yazd
City
Yazd
Province
Yazd
Postal code
8915173160
Approval date
2023-11-20, 1402/08/29
Ethics committee reference number
IR.SSU.SPH.REC.1402.105

Health conditions studied

1

Description of health condition studied
Non-alcoholic fatty liver disease (NAFLD)
ICD-10 code
K75.81
ICD-10 code description
Nonalcoholic steatohepatitis (NASH)

Primary outcomes

1

Description
Serum levels of alanine transaminase enzyme
Timepoint
Before intervention- after intervention (12 weeks after receiving barberry extract or placebo)
Method of measurement
Enzymatic method

Secondary outcomes

1

Description
Severity of hepatic steatosis
Timepoint
Before intervention- after intervention (12 weeks after receiving barberry extract or placebo)
Method of measurement
Fibroscan analysis

2

Description
Severity of liver fibrosis
Timepoint
Before intervention- after intervention (12 weeks after receiving barberry extract or placebo)
Method of measurement
Fibroscan analysis

3

Description
Severity of liver stiffness
Timepoint
Before intervention- after intervention (12 weeks after receiving barberry extract or placebo)
Method of measurement
Fibroscan analysis

4

Description
Controlled Attenuation Parameter (CAP)
Timepoint
Before intervention- after intervention (12 weeks after receiving barberry extract or placebo)
Method of measurement
Fibroscan analysis

5

Description
Serum levels of aspartate aminotransferase
Timepoint
Before intervention- after intervention (12 weeks after receiving barberry extract or placebo)
Method of measurement
Enzymatic method

6

Description
Serum levels of alkaline phosphatase
Timepoint
Before intervention- after intervention (12 weeks after receiving barberry extract or placebo)
Method of measurement
Enzymatic method

7

Description
Serum levels of gammaglutamyltransferase
Timepoint
Before intervention- after intervention (12 weeks after receiving barberry extract or placebo)
Method of measurement
Enzymatic method

Intervention groups

1

Description
Intervention group: In the intervention group, participants will receive 2 tablespoons of barberry extract (Prepared by Sanabel Daru Company) for 12 weeks. The DASH diet will be prescribed for both groups according to their characteristics and individual requirements.
Category
Treatment - Other

2

Description
Control group: In the placebo group, participants will receive a placebo syrup with a similar appearance to the intervention syrup, for 12 weeks.
Category
Placebo

Recruitment centers

1

Recruitment center
Name of recruitment center
Dr. Mohammad Hossein Ahrarizdi's clinic
Full name of responsible person
sayyed saeid khayyatzadeh
Street address
Maryam Medical building, Maryam Alley, Kashani St., Yazd
City
Yazd
Province
Yazd
Postal code
8915173160
Phone
+98 35 3623 0765
Email
khayyatzadeh@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Yazd University of Medical Sciences
Full name of responsible person
Amin Salehi abargouei
Street address
Central Administration, Bahonar Sq., Yazd
City
Yazd
Province
Yazd
Postal code
8916978477
Phone
+98 35 3628 8114
Email
dvc.research@ssu.ac.ir
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Yazd University of Medical Sciences
Proportion provided by this source
50
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

2

Sponsor
Name of organization / entity
Sanabel Daroo Company
Full name of responsible person
Dr Mohsen Naseri
Street address
Unit 1, No. 78, West Nusrat St., Tohid Square, Tehran
City
Tehran
Province
Tehran
Postal code
1457785693
Phone
+98 996 058 6908
Email
info@sanabel.ir
Web page address
https://sanabel.ir/
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Sanabel Daroo Company
Proportion provided by this source
50
Public or private sector
Private
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Industry

Person responsible for general inquiries

Contact
Name of organization / entity
Yazd University of Medical Sciences
Full name of responsible person
Sayyed Saeid Khayyatzadeh
Position
Assistant professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blvd.. Alem square
City
Yazd
Province
Yazd
Postal code
8916188637
Phone
+98 35 3820 9100
Fax
Email
khayyatzadeh@yahoo.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Yazd University of Medical Sciences
Full name of responsible person
Sayyed Saeid Khayyatzadeh
Position
Assistant professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blvd.. Alem square
City
Yazd
Province
Yazd
Postal code
8916188637
Phone
+98 35 3820 9100
Fax
Email
khayyatzadeh@yahoo.com

Person responsible for updating data

Contact
Name of organization / entity
Yazd University of Medical Sciences
Full name of responsible person
Sayyed Saeid Khayyatzadeh
Position
Assistant professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Shahid Sadoughi University of Medical Sciences, Shohadaye Gomnam Blvd.. Alem square
City
Yazd
Province
Yazd
Postal code
8916188637
Phone
+98 35 3820 9100
Fax
Email
khayyatzadeh@yahoo.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
No - There is not a plan to make this available
Statistical Analysis Plan
No - There is not a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
No - There is not a plan to make this available
Data Dictionary
No - There is not a plan to make this available
Loading...