Study aim: Assessing the effects of wet cupping therapy on some inflammatory cytokines pre- and post-treatment such as hs-CRP\ IL-6 \ IL-1β \ TNF-α Assessing the hemological and biochemical modifications pre- and post-treatment on lipid profile and liver enzymes Design: A quasi-experimental trial study with self-controls on 20 patients Settings and conduct: This quasi-experimental trial study with self-controls is conducted on 20 study subjects selected in Omid Clinic in Hamadan- Iran according to our criteria inclusion through medical examinations by our experts in Iranian Medicine Participants/Inclusion and exclusion criteria: Men and women from 20- to 60-year-old affected by non alcoholic fatty liver disease grade II and III proved by ultrasonography of the liver, with or without alteration in their liver enzymes are included in the study. Patients who were affected by other chronic liver diseases or hepatitis and anemia; also heavy smokers, alcohol drinkers and patients with prolonged intake of drugs induced fatty liver diseases are excluded from the study. Intervention groups: Wet cupping therapy every 15 days for a total of three times on all patients Main outcome variables: Modifications on inflammatory cytokines such as hs-CRP / IL-1β / IL-6 / TNF-α