History
# Registration date Revision Id
2 2020-01-22, 1398/11/02 119901
1 2017-04-14, 1396/01/25 24257
Changes made to previous revision
This is the first revision

Protocol summary

Summary
The aim of this study is evaluate the effects of probiotics on the bacterial endotoxin level of serum, as an indicator of intestinal immunity, and to compare it with the level of endotoxins in patients receiving dialysis stool. Study design: After explaining the purpose of the study and obtaining the informed consent, patients will be randomly allocated into test and control groups. Randomization will be performed using random block sizes of 4. The study is randomized, single-center, phase 3 trial. Inclusion criteria : Patients with minimum age of 18 years old and estimated glomerular filtration (GFR) rate of less than 15 ml. Exclusion criteria: Patients with active infectious conditions within the previous 30 days of the study, pregnancy; autoimmune diseases, being on immunosuppressive agents or consumption of antibiotics within 30 days prior to enrolment. Sample size: 60 patients. The intervention used in the study: one capsule of probiotics three times a week after dialysis and one capsule before bedtime in the rest of weekdays for six months. The Endotoxin level of serum and fecal samples will be then measured again and the obtained data will be analyzed.

General information

Acronym
IRCT registration information
IRCT registration number: IRCT2016103130603N1
Registration date: 2017-04-14, 1396/01/25
Registration timing: prospective

Last update:
Update count: 1
Registration date
2017-04-14, 1396/01/25
Registrant information
Name
Alireza Jafar
Name of organization / entity
Shahroud University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 23 3239 5054
Email address
alireza.jafar@shmu.ac.ir
Recruitment status
Recruitment complete
Funding source
Vice chancellor for research, Shahroud University of Medical Sciences
Expected recruitment start date
2017-04-21, 1396/02/01
Expected recruitment end date
2017-12-22, 1396/10/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
The effect of probiotics on the serum and fecal levels of endotoxin in hemodialysis patients
Public title
The effect of probiotics on infectious disease in hemodialysis patients
Purpose
Prevention
Inclusion/Exclusion criteria
Inclusion criteria: Signing a written consent to participate in the research; minimum age of 18 years old with estimated glomerular filtration rate of less than 15 ml, having a normal diet and conventional dairy. Exclusion criteria: Lack of patient's consent for participation in the research, Patients with Infectious disease; pregnancy, lactose intolerance, autoimmune or immunosuppressive disease, being on treatment with immune-suppressing drugs or anti-biotics within 30 days prior to the start of study
Age
From 18 years old
Gender
Both
Phase
3
Groups that have been masked
No information
Sample size
Target sample size: 60
Randomization (investigator's opinion)
Randomized
Randomization description
Blinding (investigator's opinion)
Not blinded
Blinding description
Placebo
Not used
Assignment
Parallel
Other design features
Reasearch will be randomised using blocked randomization design.

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Shahroud University of Medical Sciences
Street address
Haft-e-Tir Square, Shahroud, Iran
City
Shahroud
Postal code
3613773955
Approval date
2016-10-08, 1395/07/17
Ethics committee reference number
ir.shmu.rec.1395.113

Health conditions studied

1

Description of health condition studied
Chronic kidney disease
ICD-10 code
N18
ICD-10 code description
Chronic kidney disease

Primary outcomes

1

Description
Serum and fecal level of endotoxin
Timepoint
before intervenrion and at the end of intervention (after 6 months)
Method of measurement
Limulus Amebocyte Lysate (LAL) kit

Secondary outcomes

empty

Intervention groups

1

Description
Intervention Group: Use of Probiotic capsule (4BioCap 180 mg) Containing: Lactobacillus acidophilus, Bifidobacterium SPP, Streptococcus thermophilus, Lactobacillus delbrueckii bulgaricus Dosage & Duration: 6 months, 1 capsule, Daily
Category
Treatment - Drugs

2

Description
Control goup: not using probiotic capsule
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Imam Hossein's hospital of Shahroud
Full name of responsible person
Street address
Imam Hossein Square, Shahroud, Iran
City
Shahroud

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Vice Chancellor for research of Shahrood University of Medical Sciences
Full name of responsible person
Head of research
Street address
Haft-e-Tir Square, Shahroud, Iran
City
Shahroud
Grant name
-
Grant code / Reference number
-
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Vice Chancellor for research of Shahrood University of Medical Sciences
Proportion provided by this source
100
Public or private sector
empty
Domestic or foreign origin
empty
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
empty

Person responsible for general inquiries

Contact
Name of organization / entity
Shahrood University of Medical Sciences
Full name of responsible person
Alireza Jafar
Position
Medical student
Other areas of specialty/work
Street address
Haft-e-Tir Square, Shahroud, Iran
City
Shahroud
Postal code
Phone
+98 23 3239 5054
Fax
Email
mani_jafar@yahoo.com
Web page address

Person responsible for scientific inquiries

Contact
Name of organization / entity
Shahrood University of Medical Sciences
Full name of responsible person
Mehdi Mirzaii
Position
Microbiologist
Other areas of specialty/work
Street address
Haft-e-Tir Square, Shahroud, Iran
City
Shahroud
Postal code
Phone
+98 23 3239 5054
Fax
Email
mirzaii1386@gmail.com
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Shahrood University of Medical Sciences
Full name of responsible person
Alireza Jafar
Position
Medical student
Other areas of specialty/work
Street address
Haft-e-Tir Square, Shahroud, Iran
City
Shahroud
Postal code
Phone
+98 23 3239 5054
Fax
Email
mani_jafar@yahoo.com
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
empty
Study Protocol
empty
Statistical Analysis Plan
empty
Informed Consent Form
empty
Clinical Study Report
empty
Analytic Code
empty
Data Dictionary
empty
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