History
# Registration date Revision Id
2 2019-12-14, 1398/09/23 114086
1 2019-09-26, 1398/07/04 105240
Changes made to previous revision
This is the first revision

Protocol summary

Study aim
The aim of this double-blind randomized clinical trial is to determine the effects of vitamin B1, B6, B12 and folic acid supplementation versus placebo on serum levels of CGRP, Nitric oxide, homocysteine and headache characteristics in women with episodic migraine
Design
This study is a double-blind, randomized controlled clinical trial with 5 parallel groups. 100 patients will be randomly assigned into B1, B6, B12, folic acid and control group.
Settings and conduct
The study will perform at the tertiary headache clinic of Sina University Hospital. Information on demographic data and headache characteristics will be collected. After assessing the entrance criteria, 10 cc blood samples are taken. The food recall and physical activity questionnaire is completed. Anthropometric measurements and blood pressure are taken. Supplements are given to patients for a month. For double-blind implementation(patients and investigators), all boxes of supplements by a third party is coded as A, B, C, D, E.
Participants/Inclusion and exclusion criteria
Inclusion criteria: Body mass index 18.5-30, Aged 18-50 years old, Having 3-15 migraine headache attacks in last 3 months Exclusion critera: Unwillingness to participate in the study, Changes in dosage and type of migrane prophylactic drugs during intervention
Intervention groups
Intervention groups: B1, B6, B12, folic acid tablet; Control group: placebo tablet. Patients in the B1, B6, B12, folic acid group will receive 1 tablet per day, each containing 300mg, 80mg, 500mcg and 2mg ,respectively for 12 weeks. Patients in the control group will receive 1 tablets of placebo containing Methylcellulose per day.
Main outcome variables
headache attack frequency per month; headache severity (VAS); attack duration (min per month)

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20190921044828N1
Registration date: 2019-09-26, 1398/07/04
Registration timing: prospective

Last update: 2019-09-26, 1398/07/04
Update count: 1
Registration date
2019-09-26, 1398/07/04
Registrant information
Name
Shiva Nematgorgani
Name of organization / entity
Country
Iran (Islamic Republic of)
Phone
+98 21 4489 9564
Email address
shiva.gorgani@sbmu.ac.ir
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2019-10-23, 1398/08/01
Expected recruitment end date
2021-10-23, 1400/08/01
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Evaluating the effects of vitamin B1, B6, B12 and folic acid supplementation versus placebo on serum levels of CGRP, Nitric oxide, homocysteine and headache characteristics in women with episodic migraine
Public title
Evaluating the effects of vitamin B1, B6, B12 and folic acid supplementation in women with episodic migraine
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Body mass index 18.5-30 Aged 18-50 years old Migraine diagnosis according to The Classification Committee of the International Headache Society (IHS) 3rd edition (beta version) Having 3-15 migraine headache attacks in last 3 months Suffering from migraine for at least 3 months prior to study
Exclusion criteria:
Unwillingness to participate in the study Taking vitamin B group supplements in 3 months prior to study Pregnancy and lactation Taking anti-psychotic drugs Suffering from gastrointestinal disorders (malabsorption,IBD,...),diabetes, liver disorders(Cirrhosis, Hepatitis), kidney disorders(Kidney Failure), congestive heart failure, hypertension,cancer Smoking Changes in dosage and type of migrane prophylactic drugs during intervention Not taking more than 10% of supplements
Age
From 18 years old to 50 years old
Gender
Female
Phase
3
Groups that have been masked
  • Participant
  • Care provider
  • Investigator
  • Outcome assessor
  • Data analyser
Sample size
Target sample size: 100
Randomization (investigator's opinion)
Randomized
Randomization description
Patients on the basis of random allocation rule method are randomly assigned to the 5 groups; B1, B6, B12, folic acid and placebo. In this study, In this study, 20 codes for each group is placed in a drawing container and then the codes are randomly ejected from the container without replacement and the created sequences are recorded.
Blinding (investigator's opinion)
Double blinded
Blinding description
This study is a double-blind (investigators and patients) trial. For double-blind implementation, at the beginning of the study, all boxes containing B1, B6, B12, folic acid or placebo by a third party (someone other than the researchers) is coded as A, B, C, D and E.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Shahid Beheshti medical university
Street address
No. 46, West Arghavan St., Farahzadi Blv., Shahrak Qods,
City
Tehran
Province
Tehran
Postal code
1981619573
Approval date
2019-09-08, 1398/06/17
Ethics committee reference number
IR.SBMU.nnftri.Rec.1398.031

Health conditions studied

1

Description of health condition studied
migraine headache
ICD-10 code
G43
ICD-10 code description
Migraine

Primary outcomes

1

Description
migraine attack frequency per month
Timepoint
baseline-end of study
Method of measurement
headache diary

2

Description
attack severity
Timepoint
baseline-end of study
Method of measurement
headache diary-visual analogue scale

3

Description
headache attack duration (min per month)
Timepoint
baseline-end of study
Method of measurement
headache diary

Secondary outcomes

1

Description
serum homocysteine
Timepoint
baseline- end of study
Method of measurement
ELISA KIT

2

Description
serum nitric oxide
Timepoint
baseline- end of study
Method of measurement
ELISA KIT

3

Description
serum Calcitonin gene-related peptide (CGRP)
Timepoint
baseline- end of study
Method of measurement
ELISA KIT

Intervention groups

1

Description
Intervention group: 1. B1(300 mg), 2. B6 (80 mg), 3. B12 (500mcg), 4. folic acid (2mg)
Category
Treatment - Drugs

2

Description
Control group: placebo
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Sina Hospital
Full name of responsible person
Dr Mansoureh Togha
Street address
Imam Khomeini Street
City
Tehran
Province
Tehran
Postal code
1136746911
Phone
+98 21 6312 1506
Email
toghae@sina.tums.ac.ir

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Dr Esmat Naseri
Street address
No. 46, West Arghavan St., Farahzadi Blv., Shahrak Qods
City
Tehran
Province
Tehran
Postal code
1981619573
Phone
+98 21 2207 7425
Email
Nasseri_esm@yahoo.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Shahid Beheshti University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Shiva Nematgorgani
Position
Ph.D candidate
Latest degree
Master
Other areas of specialty/work
Nutrition
Street address
No. 46, West Arghavan St., Farahzadi Blv., Shahrak Qods
City
Tehran
Province
Tehran
Postal code
1981619573
Phone
+98 21 4489 9564
Email
shiva.gorgani@gmail.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Soodeh Razeghi Jahromi
Position
Assistant professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
No. 46, West Arghavan St., Farahzadi Blv., Shahrak Qods
City
Tehran
Province
Tehran
Postal code
4741-19395
Phone
+98 21 22357
Email
soodehrazeghi@gmail.com

Person responsible for updating data

Contact
Name of organization / entity
Shahid Beheshti University of Medical Sciences
Full name of responsible person
Shiva Nematgorgani
Position
Ph.D Candidate
Latest degree
Master
Other areas of specialty/work
Nutrition
Street address
No. 46, West Arghavan St., Farahzadi Blv., Shahrak Qods
City
Tehran
Province
Tehran
Postal code
1981619573
Phone
+98 21 4489 9564
Email
shiva.gorgani@gmail.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Not applicable
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Not applicable
Data Dictionary
Not applicable
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