History
# Registration date Revision Id
2 2023-11-25, 1402/09/04 287389
1 2021-01-06, 1399/10/17 166327
Changes made to previous revision
This is the first revision

Protocol summary

Study aim
To verify the non-inferior efficacy of Denosumab (AryoGen) compared with Xgeva® (produced by Amgen) by time to first on-study Skeletal Related Event (SRE) in breast cancer patients with bone metastasis
Design
A phase III, Active-controlled, Parallel, double-blind, randomized clinical trial with 272 patients.
Settings and conduct
A phase III, randomized, two armed, double-blinded, parallel, active-controlled and multicenter clinical trial in Iran
Participants/Inclusion and exclusion criteria
Inclusion: Female patients 18-75 years old, breast adenocarcinoma, Radiographic evidence of at least one bone metastasis, Eastern Cooperative Oncology Group (ECOG) ≤2, Adequate organ function. Exclusion: Planned radiation therapy or bone surgery, Life expectancy <6 months, brain and liver metastasis, Prior administration of Denosumab or IV bisphosphonates, Non-healed dental/oral problem or condition which requires oral surgery, osteonecrosis/osteomyelitis of the jaw, Disorders associated with abnormal bone metabolism, Prior malignancy (other than breast cancer, basal cell carcinoma, or in situ cervical cancer) within 3 years prior to randomization, HIV, HBV, HCV, Receiving any investigational product or device in other clinical trials 30 days prior to the study, Allergy to any of the products, Received calcitonin, Parathyroid hormone related peptides, mithramycin, strontium ranelate, or gallium nitrate within 8 weeks prior to randomization, Any psychiatric disorder, organ disfunction or systemic disease, Pregnancy or breast feeding.
Intervention groups
Control group: Denosumab (Xgeva® produced by Amgen Co), Intervention group: Denosumab (َproduced by AryoGen pharmed) (Subcutaneous, 120 mg once every 4 weeks for 80 weeks)
Main outcome variables
Assessing the time to first on-study SRE in breast cancer patients with bone metastasis.

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT20150303021315N21
Registration date: 2021-01-06, 1399/10/17
Registration timing: prospective

Last update: 2021-01-06, 1399/10/17
Update count: 1
Registration date
2021-01-06, 1399/10/17
Registrant information
Name
Nassim Anjidani
Name of organization / entity
Orchid Pharmed
Country
Iran (Islamic Republic of)
Phone
+98 21 4347 3000
Email address
amini@orchidpharmed.com
Recruitment status
Recruitment complete
Funding source
Expected recruitment start date
2021-02-03, 1399/11/15
Expected recruitment end date
2023-01-13, 1401/10/23
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
A Phase III, randomized, two armed, double-blind, parallel, active controlled, non-inferiority clinical trial to compare efficacy and safety of Denosumab (produced by AryoGen Pharmed Co.) versus Denosumab (Xgeva®, produced by Amgen Company) in breast cancer patients with bone metastasis
Public title
Evaluation of efficacy and safety of Denosumab (produced by AryoGen Pharmed Co.) versus Denosumab (Xgeva®, produced by Amgen Co.)
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Female patients aged 18-75 years old at the time of signing informed consent form ICF History or known case of breast adenocarcinoma Radiographic evidence of at least one bone metastasis Eastern Cooperative Oncology Group (ECOG) performance status of 0, 1 or 2. Adequate organ function: Albumin-adjusted serum calcium ≥ 2.0 mmol/L [≥ 8.0 mg/dL] and ≤ 2.9 mmol/L [≤ 11.5 mg/dL], Serum aspartate aminotransferase (AST) ≤2.5 ULN, Serum alanine aminotransferase (ALT) ≤2.5 ULN, Serum total bilirubin ≤2 ULN, Creatinine clearance ≥30 mL/min (stage 1-3 CKD patients), Serum Creatinine ≤1.5 ULN, Leukocytes > 3,000/mcL (without growth factor), Platelets > 100,000/mcL, Hemoglobin ≥8 g/d (with or without transfusion)
Exclusion criteria:
Planned radiation therapy or bone surgery Life expectancy less than 6 months Known brain and liver metastasis Prior administration of Denosumab or IV bisphosphonates Non-healed dental/oral surgery History or current evidence of osteonecrosis/osteomyelitis of the jaw Active dental or jaw condition which requires oral surgery Planned invasive dental procedure in the course of the study Disorders associated with abnormal bone metabolism including uncontrolled hyperthyroidism or hypothyroidism or Paget’s disease Prior malignancy (other than breast cancer, basal cell carcinoma, or in situ cervical cancer) within 3 years prior to randomization Known infection with human immunodeficiency virus (HIV) Known infection with Hepatitis B or Hepatitis C virus (HBV or HCV) Receiving any investigational product or device in other clinical trials 30 days prior to the study Allergy to any of the products to be administered during the study (eg, Denosumab, mammalian cell line products, calcium or vitamin D) Treatment with calcitonin, parathyroid hormone-related peptides, mithramycin, strontium ranelate, or gallium nitrate within 8 weeks prior to randomization Any psychiatric disorder, organ disfunction or systemic disease that, in the opinion of the investigator, might prevent the subject from completing the study or interfere with the interpretation of the study results Pregnancy or breast feeding
Age
From 18 years old to 75 years old
Gender
Female
Phase
3
Groups that have been masked
  • Participant
  • Investigator
Sample size
Target sample size: 272
Randomization (investigator's opinion)
Randomized
Randomization description
Eligible patients will be assigned to treatment with the use of stratification, permuted block (length of each block is 2), and R-CRAN software (version 3.2.3) that will be designed to achieve the overall balance between groups; randomization will be stratified according to prior Skeletal Related Event (SRE), prior oral bisphosphonate use, and current chemotherapy (chemotherapy treatment from 6 weeks prior randomization until the randomization date). After the randomization procedure, a code will be allocated to each patient that will be used as a patient identifier throughout the study. The assigned code will be denoted by 4 initials (corresponding to the 2 first letters of the first name, the 2 first letters of the first surname) and 3 numbers (center code). Moreover, the code is followed by study unique identification consisting of the first three letters of the generic name of the investigational product (which is DEN-) and 3 numbers (corresponding to the randomization number), e.g. ABCD001DEN-001. The randomization number will be assigned in a consecutive way. Each Denosumab drug packages (allocated to each patient’s injection) will have an exclusive code. Also, CRO (Contract Research Organization) will monitor the way of patient’s drug allocation in treatment groups.
Blinding (investigator's opinion)
Double blinded
Blinding description
The participants, physicians, nurse (for injection) and those who assess the study outcomes will be unaware of the state of the patient with regard to receiving Denosumab (AryoGen) or Denosumab (Xgeva®). The drug packaging type is vial, therefore the drug will be exclusively prepared in similar syringes for injection by the nurse who opens the drug packages (blinding nurse). Another nurse who injects the drug will remain blind throughout the study. Thus, the process by which the drug will be prepared, make it impossible to identify the brand of Denosumab 120 mg for the patients and investigators.
Placebo
Not used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Iran University of Medical Sciences
Street address
Iran University of Medical Sciences, Shahid Hemmat Highway, Tehran
City
Tehran
Province
Tehran
Postal code
1415935153
Approval date
2020-06-22, 1399/04/02
Ethics committee reference number
IR.IUMS.REC.1399.320

2

Ethics committee
Name of ethics committee
Shahid Beheshti University of Medical Sciences
Street address
Shahid Beheshti University, Daneshjou Boulevard, Tehran, Tehran Province
City
Tehran
Province
Tehran
Postal code
1983969411
Approval date
2020-12-03, 1399/09/13
Ethics committee reference number
IR.SBMU.CRC.REC.1399.025

Health conditions studied

1

Description of health condition studied
Breast cancer
ICD-10 code
C50.919
ICD-10 code description
Malignant neoplasm of unspecified site of unspecified female breast

Primary outcomes

1

Description
Time to first on-study Skeletal-Related Event (SRE)
Timepoint
During a 21 months period (with monthly physician visits and imaging every 3 months)
Method of measurement
The time from the date of randomization to first SRE including date of pathologic fracture, radiation or surgery to bone, or spinal cord compression

Secondary outcomes

1

Description
Time to first and subsequent (multiple) Skeletal Related Event (SRE)
Timepoint
During a 21-month period (with monthly physician visits and imaging every 3 months)
Method of measurement
The time from the date of randomization to first and subsequent SRE

2

Description
Safety Outcomes
Timepoint
During a 21-month period
Method of measurement
Safety will be assessed based on clinical examinations and laboratory test results.

3

Description
Immunogenicity
Timepoint
Weeks 0, 24, 52, 84
Method of measurement
Blood test and antidrug antibody (ADA) presence evaluation

Intervention groups

1

Description
Denosumab (produced by AryoGen Pharmed Co.), Subcutaneous, 120 mg once every 4 weeks for 80 weeks (21 doses)
Category
Treatment - Drugs

2

Description
Xgeva®(produced by Amgen Co.), Subcutaneous, 120 mg once every 4 weeks for 80 weeks (21 doses)
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Shohadaye Hafte Tir Hospital
Full name of responsible person
Dr. Pedram Fadavi
Street address
Haft-e- Tir Hospital, Shahid Rajaee St., Shahr-e-rey, Tehran
City
Shahr-e-rey
Province
Tehran
Postal code
1886719176
Phone
+98 21 5522 8581
Email
Fadavi.p@iums.ac.ir

2

Recruitment center
Name of recruitment center
Omid Hospital
Full name of responsible person
Dr. Omid Asnaashari
Street address
Omid Hospital, Farhangian Blvd., Bahonar Blvd., Urmia
City
Urmia
Province
West Azarbaijan
Postal code
5716835344
Phone
+98 44 3557 1209
Email
rahimi.noshin@gmail.com

3

Recruitment center
Name of recruitment center
Shahid Madani Hospital
Full name of responsible person
Dr. Reza Eghdam Zamiry
Street address
Shahid Madani Hospital, Daneshgah St., Tabriz
City
Tabriz
Province
East Azarbaijan
Postal code
5174745141
Phone
+98 41 3335 7767
Email
Reza-e-z@yahoo.com

4

Recruitment center
Name of recruitment center
Razi Hospital
Full name of responsible person
Dr. Hamid Saeidi Saedi
Street address
Razi Hospital, Sardarjangal Ave., Rasht
City
Rasht
Province
Guilan
Postal code
4144895596
Phone
+98 13 3354 1001
Email
H.Saeedi@tilburguniversity.edu

5

Recruitment center
Name of recruitment center
Faraz Health Clinic
Full name of responsible person
Dr. Fateme Homaei
Street address
Faraz Health Clinic, Alandasht Sq., kooh sangi St., Mashhad
City
Mashhad
Province
Razavi Khorasan
Postal code
1234567899
Phone
+98 51 3840 9183
Email
fatemeh_homaei@yahoo.com

6

Recruitment center
Name of recruitment center
Omid Hospital
Full name of responsible person
Dr. Roham Salek
Street address
Omid Hospital, Alandasht Sq., kooh sangi St., Mashhad
City
Mashhad
Province
Razavi Khorasan
Postal code
1234567891
Phone
+98 51 3842 6936
Email
salekr@mums.ac.ir

7

Recruitment center
Name of recruitment center
Emam Reza hospital
Full name of responsible person
Dr. Roham Salek
Street address
Emam Reza Hospital, Emam Reza Sq., Ibn Sina St.
City
Mashhad
Province
Razavi Khorasan
Postal code
9137913316
Phone
+98 51 3854 3031
Email
salekr@mums.ac.ir

8

Recruitment center
Name of recruitment center
Omid Hospital
Full name of responsible person
Dr. Fatemeh Varshoee Tabrizi
Street address
Omid Hospital, Alandasht Sq., kooh sangi St., Mashhad
City
Mashhad
Province
Razavi Khorasan
Postal code
1234567891
Phone
+98 51 3842 6936
Email
varshoeef@mums.ac.ir

9

Recruitment center
Name of recruitment center
Reza Radiotherapy & Oncology Center
Full name of responsible person
Dr. Fatemeh Varshoee Tabrizi
Street address
Reza Radiotherapy and Oncology Center,19 Rafsanjani St., Haftom Azar Sq., Rafsanjani Hwy., International Fair Blvd., Mashhad
City
Mashhad
Province
Razavi Khorasan
Postal code
9184166552
Phone
+98 51 3500 8530
Email
varshoeef@mums.ac.ir

10

Recruitment center
Name of recruitment center
Imam Reza Clinic
Full name of responsible person
Dr. Mansour Ansari
Street address
Imam Reza Clinic, Namazi Sq., Shiraz
City
Shiraz
Province
Fars
Postal code
7134814734
Phone
+98 71 3212 7000
Email
maansari@sums.ac.ir

11

Recruitment center
Name of recruitment center
Namazi Hospital
Full name of responsible person
Dr. Niloofar Ahmadloo
Street address
Namazi Hospital, Namazi Sq., Zand St., Shiraz
City
Shiraz
Province
Fars
Postal code
7193613311
Phone
+98 71 3647 4332
Email
ahmadloo@sums.ac.ir

12

Recruitment center
Name of recruitment center
Imam Reza Clinic
Full name of responsible person
Dr. Niloofar Ahmadloo
Street address
Imam Reza Clinic, Namazi Sq., Shiraz
City
Shiraz
Province
Fars
Postal code
7134814734
Phone
+98 71 3212 7000
Email
ahmadloo@sums.ac.ir

13

Recruitment center
Name of recruitment center
Seyed-Al-shohada Hospital
Full name of responsible person
Dr. Ali Akhavan
Street address
Seyed-Al- shohada Hospital, Farshadi Alley, Khayam St., Isfahan
City
Isfahan
Province
Isfehan
Postal code
1234567899
Phone
+98 31 3236 8005
Email
ali52akhavan@yahoo.com

14

Recruitment center
Name of recruitment center
Golestan Hospital
Full name of responsible person
Dr. Hojatallah Shahbazian
Street address
Golestan Hospital, Farvardin St., Golestan Alley, Ahvaz
City
Ahvaz
Province
Khouzestan
Postal code
6136984436
Phone
+98 61 3374 3001
Email
rahim.n.orchid@gmail.com

15

Recruitment center
Name of recruitment center
Shahid Fayaz-Bakhsh Hospital
Full name of responsible person
Dr. Hossein Foudazi
Street address
Shahid Fayaz-Bakhsh Hospital, Khalij-e-Fars Blvd., Tehran
City
Tehran
Province
Tehran
Postal code
1379613542
Phone
+98 21 6625 0645
Email
dr.foudazi@yahoo.com

16

Recruitment center
Name of recruitment center
Imam Hussein Hospital
Full name of responsible person
Dr. Robab Anbiaee
Street address
Imam Hussein Hospital, Madani St., Tehran
City
Tehran
Province
Tehran
Postal code
1617763141
Phone
+98 21 7343 3000
Email
Anbiaee@gmail

17

Recruitment center
Name of recruitment center
Imam Hussein Hospita
Full name of responsible person
Dr. Ali Yaghoubi Jouybari
Street address
Imam Hussein Hospital, Madani St., Tehran
City
Tehran
Province
Tehran
Postal code
1617763141
Phone
+98 21 7343 3000
Email
dryaghobii@yahoo.com

18

Recruitment center
Name of recruitment center
Shohadaye Tajrish Hospital
Full name of responsible person
Dr. Hamid Reza Mirzaei
Street address
Shohadaye Tajrish Hospital, Tajrish Sq., Tehran
City
Tehran
Province
Tehran
Postal code
1989934148
Phone
+98 21 2274 9201
Email
h-mirzaei@razi.tums.ac.ir

19

Recruitment center
Name of recruitment center
Haft-e- Tir Hospital
Full name of responsible person
Dr. Mastaneh Sanei
Street address
Haft-e- Tir Hospital, Shahid Rajaee St., Shahr-e-rey, Tehran
City
Shahr-e-rey
Province
Tehran
Postal code
1886719176
Phone
+98 21 5522 8581
Email
Mastanehsanei@yahoo.com

20

Recruitment center
Name of recruitment center
Mahdieh Clinic
Full name of responsible person
Dr. Negin Farshchian
Street address
Mahdieh Clinic, Moayed St., Kermanshah
City
Kermanshah
Province
Kermanshah
Postal code
6713743640
Phone
+98 83 3727 7700
Email
farshchian.n@KUMS.ac.ir

21

Recruitment center
Name of recruitment center
Sadra clinic
Full name of responsible person
Dr. Fatemeh Zahedi
Street address
Sadra clinic , Farjad building, No 19, No 10 Alley, 19th DEY St., Qom
City
Qom
Province
Ghoum
Postal code
3713615757
Phone
+98 25 3775 0582
Email
fzahedi58@yahoo.com

22

Recruitment center
Name of recruitment center
Mahdieh Hospital
Full name of responsible person
Dr. Masoumeh Keshvari
Street address
Mahdieh Hospital, Fadaeiyan Eslam St, Shoush Sq., Tehran
City
Tehran
Province
Tehran
Postal code
1185817511
Phone
+98 21 5506 2628
Email
masoumehkeshvari@yahoo.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
AryoGen Pharmed Co.
Full name of responsible person
Nassim Anjidani
Street address
Cross Tajbakhsh Street, 24th Kilometer Makhsous.
City
Garm Darreh
Province
Alborz
Postal code
56145226
Phone
+98 26 3610 6480
Email
contact@aryogen.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
AryoGen Pharmed Co.
Proportion provided by this source
100
Public or private sector
Private
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Other

Person responsible for general inquiries

Contact
Name of organization / entity
Orchid Pharmed Co.
Full name of responsible person
Nassim Anjidani
Position
Medical Department Manager
Latest degree
Medical doctor
Other areas of specialty/work
Medical Pharmacy
Street address
No 42, Attar St., Vanak Sq.
City
Tehran
Province
Tehran
Postal code
1994766411
Phone
+98 21 4347 3000
Email
anjidani.n@orchipharmed.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Iran University of Medical Sciences
Full name of responsible person
Pedram Fadavi
Position
Assistant Professor
Latest degree
Ph.D.
Other areas of specialty/work
Radiotherapy
Street address
Iran University of Medical Sciences, Shahid Hemmat Highway, Tehran
City
Tehran
Province
Tehran
Postal code
1415935153
Phone
+98 21 86701
Email
Fadavi.p@iums.ac.ir

Person responsible for updating data

Contact
Name of organization / entity
Orchid Pharmed Co.
Full name of responsible person
Nassim Anjidani
Position
Medical Department Manager
Latest degree
Medical doctor
Other areas of specialty/work
Medical Pharmacy
Street address
No 42, Attar Neyshaboori St., Vanak Sq.
City
Tehran
Province
Tehran
Postal code
1994766411
Phone
+98 21 4347 3000
Email
anjidani.n@orchidpharmed.com

Sharing plan

Deidentified Individual Participant Data Set (IPD)
Undecided - It is not yet known if there will be a plan to make this available
Study Protocol
Undecided - It is not yet known if there will be a plan to make this available
Statistical Analysis Plan
Undecided - It is not yet known if there will be a plan to make this available
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Undecided - It is not yet known if there will be a plan to make this available
Analytic Code
Undecided - It is not yet known if there will be a plan to make this available
Data Dictionary
Undecided - It is not yet known if there will be a plan to make this available
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