History
# Registration date Revision Id
3 2019-09-26, 1398/07/04 105239
2 2018-02-28, 1396/12/09 39764
1 2015-09-27, 1394/07/05 6083
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  • Protocol summary

    Objective: The aim of the current study is to evaluate the effects of synbiotic supplementation on metabolic profiles and biomarkers of oxidative stress in women with polycystic ovary syndrome (PCOS). Study design: Parallel double-blind (both patients and researchers) randomized controlled clinical trial. Random assignment was done by the use of computer-generated random numbers. Inclusion and exclusion criteria: In the current study, women aged 18-40 years diagnosed with PCOS will be included. Unwillingness to cooperate and the use of antibiotic medications will be excluded in the study. Population and sample size: 60 patients with PCOS among women of eligible and referred to Kosar Clinic affiliated to Arak University of Medical Sciences, Arak, Iran in the study will be selected. Intervention: Patients will be assigned to receive either synbiotic supplements (intervention group: n=30) or placebo (control group: n=30). Fasting blood samples will be taken at baseline and after 12 weeks of intervention to measure metabolic profiles. Start and end date of intervention: 12 weeks (September 1, 2015-December 1, 2015). Outcomes: Metabolic profiles and biomarkers of oxidative stress will be measured at study baseline and after 12 weeks of intervention.
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    هدف: هدف مطالعه حاضر ارزیابی اثرات مکمل یاری سین بیوتیک بر پروفایل های متابولیک و بیومارکرهای استرس اکسیداتیو در زنان مبتلا به سندروم تخمدان پلی کیستیک است. طراحی مطالعه: تصادفي کارآزمایی بالینی دو سوکور (هم بیماران و هم محققان) موازی. تصادفی سازی با استفاده از اعداد تصادفی ایجاد شده توسط کامپیوتر انجام می شود. معیار ورود و خروج مطالعه: در مطالعه حاضر، زنان 40-18 ساله مبتلا به سندروم تخمدان پلی کیستیک وارد خواهند شد. عدم تمایل به همکاری و مصرف داروهای آنتی بیوتیک از مطالعه خارج خواهند شد. حجم نمونه و جمعیت مورد مطالعه: 60 بیمار مبتلا به سندروم تخمدان پلی کیستیک در میان زنان واجد شرایط و ارجاع شده به کلینیک کوثر وابسته به دانشگاه علوم پزشکی اراک، اراک، ایران در مطالعه انتخاب خواهند شد. مداخله مورد مطالعه: بیماران برای دریافت مکمل سین بیوتیک (گروه مداخله: 30n=) یا پلاسبو (گروه کنترل30n=) تقسیم خواهند شد. نمونه خون ناشتا در ابتدا و 12 هفته بعد از مداخله برای تعیین پروفایل های متابولیک گرفته خواهد شد. زمان شروع و اتمام مداخله: 12 هفته (از 10 شهریور 1394 تا 10 آذر 1394). پيامدها: پروفایل های متابولیک و بیومارکرهای استرس اکسیداتیو در ابتدا و 12 هفته بعد از مداخله اندازه گیری خواهد شد.
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    The aim of the current study is to evaluate the effects of synbiotic supplementation on metabolic profiles and biomarkers of oxidative stress in women with polycystic ovary syndrome (PCOS).
    empty
    هدف مطالعه حاضر ارزیابی اثرات مکمل یاری سین بیوتیک بر پروفایل های متابولیک و بیومارکرهای استرس اکسیداتیو در زنان مبتلا به سندروم تخمدان پلی کیستیک است.
    empty
    Random assignment was done by the use of computer-generated random numbers.Population and sample size: 60 patients with PCOS among women of eligible and referred to Kosar Clinic affiliated to Arak University of Medical Sciences, Arak, Iran in the study will be selected.
    empty
    تصادفی سازی با استفاده از اعداد تصادفی ایجاد شده توسط کامپیوتر انجام می شود. حجم نمونه و جمعیت مورد مطالعه: 60 بیمار مبتلا به سندروم تخمدان پلی کیستیک در میان زنان واجد شرایط و ارجاع شده به کلینیک کوثر وابسته به دانشگاه علوم پزشکی اراک، اراک، ایران در مطالعه انتخاب خواهند شد.
    empty
    60 patients with PCOS among women of eligible and referred to Kosar Clinic affiliated to Arak University of Medical Sciences, Arak, Iran in the study will be selected.
    empty
    60 بیمار مبتلا به سندروم تخمدان پلی کیستیک در میان زنان واجد شرایط و ارجاع شده به کلینیک کوثر وابسته به دانشگاه علوم پزشکی اراک، اراک، ایران در مطالعه انتخاب خواهند شد.
    empty
    In the current study, women aged 18-40 years diagnosed with PCOS will be included. Unwillingness to cooperate and the use of antibiotic medications will be excluded in the study.
    empty
    معیار ورود و خروج مطالعه: در مطالعه حاضر، زنان 40-18 ساله مبتلا به سندروم تخمدان پلی کیستیک وارد خواهند شد. عدم تمایل به همکاری و مصرف داروهای آنتی بیوتیک از مطالعه خارج خواهند شد.
    empty
    Patients will be assigned to receive either synbiotic supplements (intervention group: n=30) or placebo (control group: n=30).
    empty
    بیماران برای دریافت مکمل سین بیوتیک (گروه مداخله: 30n=) یا پلاسبو (گروه کنترل30n=) تقسیم خواهند شد.
    empty
    Outcomes: Metabolic profiles and biomarkers of oxidative stress will be measured at study baseline and after 12 weeks of intervention.
    empty
    پروفایل های متابولیک و بیومارکرهای استرس اکسیداتیو در ابتدا و 12 هفته بعد از مداخله اندازه گیری خواهد شد.
  • General information

    1
    1
    Women aged 18-40 years
    Diagnosed with PCOS
    زنان 40-18 ساله
    تشخیص داده شده به سندروم تخمدان پلی کیستیک
    Unwillingness to cooperate
    The use of antibiotic medications
    عدم تمایل به همکاری
    مصرف داروهای آنتی بیوتیک
    empty
    Simple randomization
    empty
    تصادفی سازی ساده
    empty
    Placebo has been used to achieve masking of participants and investigators. The researchers and patients will be concealed until the final analyses by a trained staff.
    empty
    از پلاسبو برای کور سازی استفاده شده است. محققان و بیماران کور تا زمان آنالیز نهایی توسط یک کارمند آموزش دیده خواهند شد.
  • Health conditions studied

    #1
    Polycystic ovary syndrome
    Polycystic ovarian syndrome
  • Primary outcomes

    #1
    Insulin
    Hs-CRP
    انسولین
    پروتئین واکنشگر C با حساسیت بالا
    #2
    Insulin resistance
    Nitric oxide
    مقاومت به انسولین
    نیتریک اکساید
    Elisa kit
    Spectrophotometry
    کیت الایزا
    اسپکتروفتومتری
  • Secondary outcomes

    #1
    Triglycerides
    Serum dehydroepiandrosterone
    تری گلیسرید
    دی هیدرواپی آندرسترون سرمی
    Enzymatic kit
    Elisa kit
    کیت آنزیمی
    کیت الایزا
    #2
    Total cholesterol
    Serum total testosterone
    کلسترول تام
    توتال تستوسترون سرمی
    Enzymatic kit
    Elisa kit
    کیت آنزیمی
    کیت الایزا
    #3
    HDL-cholesterol
    Plasma malondialdehyde
    کلسترول HDL
    مالون دی آلدئید پلاسمایی
    Enzymatic kit
    Spectrophotometry
    کیت آنزیمی
    اسپکتروفتومتری
    #4
    Total antioxidant
    Plasma total antioxidant capacity
    آنتی اکسیدانت توتال
    آنتی اکسیدانت توتال پلاسمایی
    #5
    Glutathione
    Plasma glutathione
    گلوتاتیون
    گلوتاتیون پلاسمایی
  • Recruitment centers

    #1
    Name of recruitment center - English: Kosar Clinic
    Name of recruitment center - Persian: کلینیک کوثر
    Full name of responsible person - English: Khadijeh Nasri
    Full name of responsible person - Persian: خدیجه نصری
    Street address - English: Emam Khomeyni Avenue, Arak
    Street address - Persian: اراک، خیابان امام خمینی
    City - English: Arak
    City - Persian: اراک
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:
    Name of recruitment center - English: Kosar Clinic
    Name of recruitment center - Persian: کلینیک کوثر
    Full name of responsible person - English: Khadijeh Nasri
    Full name of responsible person - Persian: خدیجه نصری
    Street address - English: Emam Khomeyni Avenue, Arak
    Street address - Persian: اراک، خیابان امام خمینی
    City - English: Arak
    City - Persian: اراک
    Province: Markazi
    Country: Iran (Islamic Republic of)
    Postal code: 8715988141
    Phone: +98 86 2769 3000
    Fax:
    Email: khadijeh.nasri2@gmail.com
    Web page address:
  • Sponsors / Funding sources

    #1
    contact.organization_id:
    Name of organization / entity - English: Vice chancellor for research, Arak University of Medical Sciences
    Name of organization / entity - Persian: معاونت پژوهشی دانشگاه علوم پزشکی اراک
    Full name of responsible person - English: Mohammad Rafiee
    Full name of responsible person - Persian: محمد رفیعی
    Street address - English: Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
    Street address - Persian: اراك، خیابان سردشت، معاونت آموزش و تحقيقات
    City - English: Arak
    City - Persian: اراک
    Province:
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone:
    Fax:
    Email:
    Web page address:

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Mohammad Rafiee
    Full name of responsible person - Persian: محمد رفیعی
    Street address - English: Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
    Street address - Persian: اراك، خیابان سردشت، معاونت آموزش و تحقيقات
    City - English: Arak
    City - Persian: اراک
    Province: Markazi
    Country: Iran (Islamic Republic of)
    Postal code: 8715988141
    Phone: +98 86 3276 9300
    Fax:
    Email: rafiee.mohammad22@gmail.com
    Web page address:
    empty
    Public
    empty
    Domestic
    empty
    Academic
    Vice chancellor for research, Arak University of Medical Sciences
    Arak University of Medical Sciences
    معاونت پژوهشی دانشگاه علوم پزشکی اراک
    دانشگاه علوم پزشکی اراک
  • Person responsible for general inquiries

    contact.organization_id:
    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English:
    Full name of responsible person - Persian:
    Position - English:
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    Latest degree:
    Area of specialty/work:
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    Street address - English:
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    City - English:
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    Province:
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    Province - Persian:
    contact.provinces_available:
    Country:
    Postal code:
    Phone:
    Mobile:
    Fax:
    Email:
    Web page address:

    Name of organization / entity - English:
    Name of organization / entity - Persian:
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: Associate professor
    Position - Persian: دانشیار
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 8715988141
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_r@yahoo.com
    Web page address:
  • Person responsible for scientific inquiries

    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکترای تغذیه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir-asemi_r@yahoo.com
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکترای تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 8715988141
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir
    Web page address:
  • Person responsible for updating data

    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree:
    Area of specialty/work: 0
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province:
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code:
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir-asemi_r@yahoo.com
    Web page address:
    contact.organization_id:
    Name of organization / entity - English: Kashan University of Medical Sciences
    Name of organization / entity - Persian: دانشگاه علوم پزشکی کاشان
    Full name of responsible person - English: Zatollah Asemi
    Full name of responsible person - Persian: ذات اله عاصمی
    Position - English: PhD of Nutrition
    Position - Persian: دکتری تغذیه
    Latest degree: phd
    Area of specialty/work: 34
    Area of specialty/work title - English:
    Area of specialty/work title - Persian:
    Street address - English: Ghotbe Ravandi Boulevard, Kashan
    Street address - Persian: کاشان، بلوار قطب راوندی
    City - English: Kashan
    City - Persian: کاشان
    Province: Isfehan
    Province - English:
    Province - Persian:
    contact.provinces_available: 1
    Country: Iran (Islamic Republic of)
    Postal code: 8715988141
    Phone: +98 31 5546 3378
    Mobile: +98 913 361 5446
    Fax:
    Email: asemi_z@kaums.ac.ir
    Web page address:
  • Sharing plan

    no
    no
    na
    undecided
    na
    na
    na
    empty
    Not
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    خیر

Protocol summary

Study aim
The aim of the current study is to evaluate the effects of synbiotic supplementation on metabolic profiles and biomarkers of oxidative stress in women with polycystic ovary syndrome (PCOS).
Design
Random assignment was done by the use of computer-generated random numbers.Population and sample size: 60 patients with PCOS among women of eligible and referred to Kosar Clinic affiliated to Arak University of Medical Sciences, Arak, Iran in the study will be selected.
Settings and conduct
60 patients with PCOS among women of eligible and referred to Kosar Clinic affiliated to Arak University of Medical Sciences, Arak, Iran in the study will be selected.
Participants/Inclusion and exclusion criteria
In the current study, women aged 18-40 years diagnosed with PCOS will be included. Unwillingness to cooperate and the use of antibiotic medications will be excluded in the study.
Intervention groups
Patients will be assigned to receive either synbiotic supplements (intervention group: n=30) or placebo (control group: n=30).
Main outcome variables
Outcomes: Metabolic profiles and biomarkers of oxidative stress will be measured at study baseline and after 12 weeks of intervention.

General information

Reason for update
Acronym
IRCT registration information
IRCT registration number: IRCT201509115623N53
Registration date: 2015-09-27, 1394/07/05
Registration timing: retrospective

Last update: 2018-03-10, 1396/12/19
Update count: 2
Registration date
2015-09-27, 1394/07/05
Registrant information
Name
Zatollah Asemi
Name of organization / entity
Kashan University of Medical Sciences
Country
Iran (Islamic Republic of)
Phone
+98 36 1534 3570
Email address
asemi_z@kaums.ac.ir
Recruitment status
Recruitment complete
Funding source
Arak University of Medical Sciences
Expected recruitment start date
2015-08-23, 1394/06/01
Expected recruitment end date
2015-09-01, 1394/06/10
Actual recruitment start date
empty
Actual recruitment end date
empty
Trial completion date
empty
Scientific title
Clinical trial of the effect of synbiotic supplementation compared with the placebo on metabolic profiles and biomarkers of oxidative stress in women with polycystic ovary syndrome
Public title
Effect of supplementation in treatment of women with polycystic ovary syndrome
Purpose
Treatment
Inclusion/Exclusion criteria
Inclusion criteria:
Women aged 18-40 years Diagnosed with PCOS
Exclusion criteria:
Unwillingness to cooperate The use of antibiotic medications
Age
From 18 years old to 40 years old
Gender
Female
Phase
2
Groups that have been masked
  • Participant
  • Investigator
Sample size
Target sample size: 60
Randomization (investigator's opinion)
Randomized
Randomization description
Simple randomization
Blinding (investigator's opinion)
Double blinded
Blinding description
Placebo has been used to achieve masking of participants and investigators. The researchers and patients will be concealed until the final analyses by a trained staff.
Placebo
Used
Assignment
Parallel
Other design features

Secondary Ids

empty

Ethics committees

1

Ethics committee
Name of ethics committee
Ethics committee of Arak University of Medical Sciences
Street address
Arak University of Medical Sciences
City
Arak
Province
Markazi
Postal code
8715988141
Approval date
2015-08-23, 1394/06/01
Ethics committee reference number
IR.ARAKMU.REC.1394.160

Health conditions studied

1

Description of health condition studied
Polycystic ovarian syndrome
ICD-10 code
E28.2
ICD-10 code description
Polycystic ovarian syndrome

Primary outcomes

1

Description
Hs-CRP
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Elisa kit

2

Description
Nitric oxide
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Spectrophotometry

Secondary outcomes

1

Description
Fasting blood sugar
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Enzymatic kit

2

Description
Serum dehydroepiandrosterone
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Elisa kit

3

Description
Serum total testosterone
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Elisa kit

4

Description
Plasma malondialdehyde
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Spectrophotometry

5

Description
Plasma total antioxidant capacity
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Spectrophotometry

6

Description
Plasma glutathione
Timepoint
At the beginning of the study and after 12 weeks of intervention
Method of measurement
Spectrophotometry

Intervention groups

1

Description
Intervention group: Synbiotic capsule, Lactobacillus acidophilus (2×109 CFU/g), Lactobacillus casei (2×109 CFU/g) and Bifidobacterium bifidum (2×109 CFU/g) plus 0.8 g inulin, daily, for 12 weeks orally.
Category
Treatment - Drugs

2

Description
Control group: Placebo capsule, daily, for 12 weeks orally.
Category
Treatment - Drugs

Recruitment centers

1

Recruitment center
Name of recruitment center
Kosar Clinic
Full name of responsible person
Khadijeh Nasri
Street address
Emam Khomeyni Avenue, Arak
City
Arak
Province
Markazi
Postal code
8715988141
Phone
+98 86 2769 3000
Email
khadijeh.nasri2@gmail.com

Sponsors / Funding sources

1

Sponsor
Name of organization / entity
Arak University of Medical Sciences
Full name of responsible person
Mohammad Rafiee
Street address
Vice chancellor for research, Arak University of Medical Sciences, Sardasht Avenue, Arak
City
Arak
Province
Markazi
Postal code
8715988141
Phone
+98 86 3276 9300
Email
rafiee.mohammad22@gmail.com
Grant name
Grant code / Reference number
Is the source of funding the same sponsor organization/entity?
Yes
Title of funding source
Arak University of Medical Sciences
Proportion provided by this source
100
Public or private sector
Public
Domestic or foreign origin
Domestic
Category of foreign source of funding
empty
Country of origin
Type of organization providing the funding
Academic

Person responsible for general inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
Associate professor
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5546 3378
Email
asemi_r@yahoo.com

Person responsible for scientific inquiries

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5546 3378
Fax
Email
asemi_z@kaums.ac.ir
Web page address

Person responsible for updating data

Contact
Name of organization / entity
Kashan University of Medical Sciences
Full name of responsible person
Zatollah Asemi
Position
PhD of Nutrition
Latest degree
Ph.D.
Other areas of specialty/work
Nutrition
Street address
Ghotbe Ravandi Boulevard, Kashan
City
Kashan
Province
Isfehan
Postal code
8715988141
Phone
+98 31 5546 3378
Fax
Email
asemi_z@kaums.ac.ir
Web page address

Sharing plan

Deidentified Individual Participant Data Set (IPD)
No - There is not a plan to make this available
Justification/reason for indecision/not sharing IPD
Not
Study Protocol
No - There is not a plan to make this available
Statistical Analysis Plan
Not applicable
Informed Consent Form
Undecided - It is not yet known if there will be a plan to make this available
Clinical Study Report
Not applicable
Analytic Code
Not applicable
Data Dictionary
Not applicable
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